Investigation of the toxicity of ethanol extracts obtained from six different Satureja L. species on Colorado Potato Beetle, Leptinotarsa decemlineata (Say, 1824), (Coleoptera: Chrysomelidae)

Year 2019, , 69 - 79, 01.11.2019

Ayşe Usanmaz Bozhüyük , Şaban Kordali

https://doi.org/10.30616/ajb.623827

Cited By: 5

Abstract

In the
present study, ethanol extracts obtained from Satureja cilicica P. H. Davis,
Satureja cuneifolia Ten, Satureja
hortensis L., Satureja spicigera (C.
Koch) Boiss., Satureja thymbra L. and
Satureja montana L. were tested against on the adults and larvae of
Colorado potato beetle (Leptinotarsa
decemlineata (Say, 1824)). The experiments were conducted in glass Petri
dishes and vacuum desiccators including 15 individual for each period with
three replicates under laboratory conditions. 10, 15 and 20 mg/mL doses of ethanol extracts conducted in the Petri dishes and the
desiccators showed that depending on concentration increase and duration of
exposure time resulted between 2.22-100% toxic effects on the potato beetle
larvae and adults. In Petri trials, the highest mortality rate was recorded as
100% for the second larval stage at the 20 mg/mL dose of S. spicigera ethanol extract after 96
hours the treatment. In desiccator experiments, the highest toxicity rate was
determined as 100% for first larval stage at the 20 mg/mL dose of S. thymbra ethanol
extract after 96 hours of the application. In addition to, when LD values of
the ethanol extracts ​​were taken into account the highest toxicity of adult
period was determined for S. thymbra extract (LD25:0.000, LD50:
0.010 μL/insect), the lowest toxicity was determined for S. cilicica extract (LD90:
436.020 μL/insect). The results obtained from this study suggested that the
ethanol extracts of tested Satureja L.
species could be used for L. decemlineata
larvae and adults as bio-larvicides and insecticides.

Keywords

Ethanol extracts, Leptinotarsa decemlineata, Satureja species, toxic effect

Supporting Institution

Ataturk University Scientific Research Projects

Project Number

BAP 2012/233

Thanks

This study is a part of master thesis supported by Ataturk University Scientific Research Projects (BAP 2012/233). The authors would like to thank Assoc. Prof. Dr. Memiş KESDEK (Mugla Sıtkı Kocman University) for valuable contributions and their helpful comments on the earlier versions of this manuscript.

Altı farklı Satureja L. türünden elde edilen etanol ekstraktının Patates Böceği, Leptinotarsa decemlineata (Say, 1824), (Coleoptera: Chrysomelidae) üzerindeki toksisitelerinin araştırılması

Abstract

Bu
çalışmada, Satureja cilicica P. H.
Davis, Satureja cuneifolia Ten, Satureja hortensis L., Satureja spicigera (C. Koch) Boiss., Satureja thymbra L. ve Satureja montana L. bitkilerinden elde
edilen ethanol ekstraktları patates böceğinin ergin ve larvaları üzerinde test
edilmiştir. Testler laboratuvar koşulları altında cam Petri ve vakumlu
desikatörlere yerleştirilmiş her bir döneme ait 15 bireyde 3 tekerrürlü olarak
yapılmıştır. Petri ve desikatör denemelerinde ethanol ekstraklarının 10, 15 ve
20 mg/mL’lik dozları konsantrasyon artışına ve maruz kalma süresine bağlı olarak
patates böceği larva dönemleri ve erginleri üzerinde 2.22-100% oranında toksik
etki göstermiştir. Petri denemelerinde, en yüksek ölüm oranı uygulanmadan 96 saat
sonra S. spicigera ethanol
ekstraktının 20 mg/mL’lik dozunda ikinci larva döneminde %100 olarak
kaydedilmiştir. Desikatör
denemelerinde ise, en yüksek toksisite oranı uygulamadan 96 saat sonra S. thymbra etanol ekstraktının 20 mg/mL’lik
dozunda birinci larva dönemi için % 100 olarak belirlenmiştir. Ek olarak,
etanol ekstraktlarının LD değerleri dikkate alındığında, en yüksek toksitite ergin dönemde S. thymbra ekstraktında
(LD25:0.000, LD50:
0.010 μL/böcek), en düşük toksitite ise S.
cilicica ekstraktında (LD90: 436.020 μL/böcek) olarak
belirlenmiştir. Bu
çalışmadan elde edilen sonuçlar, test edilen Satureja L. türleri etanol ekstraklarının L. decemlineata larvaları ve yetişkinleri için biyo-larvisit ve
insektisit olarak kullanılabileceğini göstermiştir.

Keywords

Etanol ekstraktı, Leptinotarsa decemlineata, Satureja türleri, toksik etki

Project Number

BAP 2012/233

References

• Alkan M, Gökçe A, Kara K (2015). Antifeedant activity and growth inhibition effects of some plant extracts against larvae of Colorado Potato Beetle [Leptino¬tarsa decemlineata Say (Col: Chyrsomelidae)] under laboratory conditions. Turkish Journal of Entomology 39(4): 345–353.
• Barnard C, Padgitt M, Uri ND (1997). Pesticide use and its measurement. International Pest Control (United Kingdom) 39: 161–164.
• Bashır M, Gogi MD, Ashfaq M, Afzal DM, Khan MA, Ihsan M (2013). The efficacy of crude aqueous extracts of some plants as grain protectants against the stored grain mite, Rhizoglyphus tritici. Turkish Journal of Agriculture and Forestry 37(5): 585-594.
• Başer KHC, Tümen G, Tabanca N, Demirci F (2001). Composition and antibacterial activity of the essential oils from Satureja wiedemanniana (Lallem.) Velen. Zeitschrift für Naturforschung C 56(9-10): 731-738.
• Biniaś B, Gospodarek J (2017). Effect of water extract from chamomile on black bean aphid and Colorado Potato Beetle. Journal of Ecological Engineering 18(3).
• Chiasson H, Belanger A, Bostanian N, Vincent C, Poliquin A (2001). Acaricidal properties of Artemisia absinthium and Tanacetum vulgare (Asteraceae) essential oils obtained by three methods of extraction. Journal of Economic Entomology 94: 167–171.
• Cronquist A (1988). The evolution and classification of flower plants. The New York Botanical Garden, Bronx, NY.
• Duru ME, Çakir A, Kordali S, Zengin H, Harmandar M, Izumi S, Hirata T (2003). Chemical composition and antifungal properties of essential oils of three Pistacia species. Fitoterapia 74: 170-176.
• Emsen B, Bulak Y, Yildirim E, Aslan A, Ercisli S (2012). Activities of two major lichen compounds, diffractaic acid and usnic acid against Leptinotarsa decemlineata Say, 1824 (Coleoptera: Chrysomelidae). Egyptian Journal of Biological Pest Control 22(1): 5-10.
• Ferro DN, Morzuch BJ, Margolies D (1983). Crop loss assessment of the Colorado Potato Beetle (Coleoptera: Chrysomelidae) on potatoes in Western Massachusetts. Journal Economic Entomology 76: 349-356.
• Finney DJ (1971). Probit analysis 3rd edition. London: Cambridge Univ. Press.
• Gelman DB, Bell RA, Liska LJ, Hu JS (2001). Artificial diets for rearing the Colorado Potato Beetle, Leptinotarsa decemlineata. Journal of Insect Science 1(1): 7.
• Gokturk T, Kordali Ş, Bozhüyük AU (2017). Insecticidal effect of essential oils against Fall Webworm (Hypantria cunea Drury (Lepidoptera: Arctiidae)). Natural Product Communications, 12 (10): 1659-1662.
• González-Coloma A, Guadano A, Gutiérrez C, Cabrera R, De La Pena E, De La Fuente G, Reina M (1998). Antifeedant Delphinium Diterpenoid Alkaloids. Structure-activity relationships. Journal of Agricultural and Food Chemistry 46(1): 286-290.
• Gonzalez-Coloma A, Reina M, Cabrera R, Castañera P, Gutierrez C (1995). Antifeedant and toxic effects of sesquiterpenes from Senecio palmensis to Colorado Potato Beetle. Journal of Chemical Ecology 21(9): 1255-1270.
• González-Coloma A, Reina M, Medinaveitia A, Guadaño A, Santana O, Martínez-Díaz R, Gavín JA (2004). Structural diversity and defensive properties of norditerpenoid alkaloids. Journal of Chemistry Ecology 30(7):1393-1408.
• González-Coloma A, Valencia F, Martín N, Hoffmann JJ, Hutter L, Marco JA, Reina M (2002). Silphinene sesquiterpenes as model insect antifeedants. Journal of Chemistry Ecology 28(1): 117-129.
• Gökçe A, Whalon ME, Çam H, Yanar Y, Demirtas İ, Goren N (2006). Contact and residual toxicities of 30 plant extracts to Colorado Potato Beetle Larvae. Archives of Phytopathology and Plant Protection 1:10.
• Güllüce M, Sökmen M, Daferera D, Aǧar G, Özkan H, Kartal N, Polissiou M, Sahin F (2003). In vitro antibacterial, antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agricultural and Food Chemistry 51(14): 3958-3965.
• Güzel S, Pavela R, Ilcim A, Kökdil G (2017). Phytochemical composition and antifeedant activity of five Vincetoxicum taxa against Spodoptera littoralis and Leptinotarsa decemlineata. Marmara Pharmaceutical Journal 21(4): 872-880.
• Hadian J, Ebrahimi SN, Salehi P (2010). Variability of morphological and phytochemical characteristics among Satureja hortensis L. accessions of Iran. Industrial Crops and Products 32(1): 62-69.
• Hoffmann MP, Frodsham AC (1993). Natural enemies of vegetable insect pests. Ithica: Comel University Press.
• Hough-Goldstein JA (1990). Antifeedant effects of common herbs on the Colorado Potato Beetle (Coleoptera: Chrysomelidae). Environmental Entomology 19(2): 234-238.
• Hu JS, Gelman DB, Bell RA (1999). Effects of selected physical and chemical treatments of Colorado Potato Beetle eggs on host acceptance and development of the parasitic wasps. Edovum Puttleri. Entomologia Experimentalis Applicata 90: 237-245.
• Isman MB (2000). Plant essential oils for pest and disease management. Crop Protection 19: 603-608.
• Kara N, Yorulmaz Salman S, Baydar H, 2014. The usage of sage (Salvia officinalis L.) and rosemary (Rosmarinus officinalis L.) extracts in the nanagement of Potato Beetle (Leptinotarsa decemlineata Say.). Turkish Journal of Agriculture and Natural Science 1(2): 248-254.
• Kesdek M, Kordali S, Coban K, Usanmaz A, Ercisli S (2014). Larvicidal effect of some plant extracts on the pine processionary moth, Thaumetopoea pityocampa (Denis & Schiffermuller) in laboratory conditions. Acta Scientiarum Polonorum Hortorum Cultus 13(5).
• Kesdek M, Kordali S, Usanmaz Bozhuyuk A, Ercisli S (2015). The toxicity of essential oils of some plant species against adults of Colorado Potato Beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Comptes Rendus de l’Académie Bulgare Des Sciences 68(1): 127-136.
• Kısa A, Akyüz M, Çoğun HY, Kordali Ş, Bozhüyük AU, Tezel B, Çakır A (2018). Effects of Olea europaea L. leaf metabolites on the tilapia (Oreochromis niloticus) and three stored pests, Sitophilus granarius, Tribolium confusum and Acanthoscelides obtectus. Records of Natural Products 12(3): 201.
• Kordali S, Cakir A, Akcin TA, Mete E, Akcin A, Aydin T, Kilic H (2009). Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan.(Asteraceae). Industrial Crops and Products 29(2-3):562-570.
• Kordali S, Kesdek M, Cakir A (2007a). Toxicity of monoterpenes against larvae and adults of Colorado Potato Beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Industrial Crops and Products 26: 278-297.
• Kordali S, Kotan R, Cakir A (2007b). Screening of in vitro antifungal activities of 21 oxygenated monoterpenes in vitro as plant disease control agents. Allelopathy Journal 19(2): 373-391.
• Kordali Ş, Çakır A, Özer H, Çakmakcı R, Kesdek M, Mete E (2008). Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and P-cymene. Bioresource Technology 99: 8788-8795.
• Lee S, Peterson CJ, Coats JR (2003). Fumigation toxicity of monoterpenoids to several stored product insects. Journal of Stored Products Research 39(1): 77-85.
• Michaelakis A, Theotokatos SA, Koliopoulos G, Chorianopoulos NG (2007). Essential oils of Satureja species: Insecticidal effect on Culex pipiens larvae (Diptera: Culicidae). Molecules 12: 2567-2578.
• Momtaz S, Abdollahi M (2010). An update on pharmacology of Satureja species; from antioxidant, antimicrobial, antidiabetes and anti-hyperlipidemic to reproductive stimulation. International Journal of Pharmacology 6(4), 346-353.
• Oerke, EC, Dehne HW, Schonbeck F, Weber A (1994). Crop production and crop protection estimated losses in major food and cash crops. Elsevier BV.
• Öztekin M (2012). Satureja L. A checklist of the Flora of Turkey (Vascular Plants). Editors: Güner A, Aslan S, Ekim T, Vural M, Babaç MT. İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği.
• Pavela R (2010). Antifeedant activity of plant extracts on Leptinotarsa decemlineata Say. and Spodoptera littoralis Bois. larvae. Industrial Crops and Products 32(3): 213-219.
• Popova EN (2014). The Influence of Climatic Changes on range expansion and phenology of the Colorado potato beetle (Leptinotarsa decemlin¬eata, Coleoptera, Chrysomelidae) in the territory of Russia. Entomological Review 94(5): 643-653.
• Rusin M, Gospodarek J, Biniaś B (2015). The effect of water extracts from Artemisia absinthium L. on feeding of Leptinotarsa decemlineata Say. larvae. Journal of Research and Applications in Agricultural Engineering 60(4): 80-83.
• Scott IM, Jensen H, Nicol R, Lesage L, Bradbury R, Sanchez-Vindas P, Poveda L, Arnason JT, Philogene BJR (2004). Efficacy of Piper (Pipeaceae) extracts for control of common home and garden insect pests. Journal of Economic Entomology 97(4): 1390-1403.
• Scott IM, Jensen H, Scott JG, Isman MB, Arnason JT, Philogene BJR (2003). Botanical insecticides for controlling agricultural pests: Piperamides and the Colorado Potato Beetle Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Archives of Insect Biochemistry and Physiology: Published in Collaboration with the Entomological Society of America 54(4): 212-225.
• Szendrei Z, Grafius E, Byrne A, Ziegler A (2012). Resistance to neonicotinoid insecticides in field populations of the Colorado potato beetle (Coleoptera: Chrysomelidae). Pest Management Science 68(6):941-946.
• Tampe J, Parra L, Huaiquil K, Mutis A, Quiroz A (2015). Repellent effect and metabolite volatile profile of the essential oil of Achillea millefolium against Aegorhinus nodipennis (Hope)(Coleoptera: Curculionidae). Neotropical entomology 44(3):279-285.
• Topuz E, Madanlar N, Erler F (2018). Chemical composition, toxic and development and reproduction inhibiting effects of some essential oils against Tetranychus urticae Koch (Acarina: Tetranychidae) as fumigants. Journal of Plant Diseases and Protection 125(4): 377-387.
• Usanmaz Bozhuyuk A, Kordali S, Kesdek M, Altinok MA, Ercisli S (2016). Toxic effects of eight plant essential oils against adults of Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Egyptian Journal of Biological Pest Control 26(3): 439.
• Yıldırım E, Kesdek M, Aslan İ, Çalmaşur Ö, Şahin F (2005). The effects of essential oils from eight plant species on two pests of stored product insects. Fresenius Environmental Bulletin 14: 23– 27.
• Zarshenas MM, Krenn L (2015). Phytochemical and pharmacological aspects of Salvia mirzayanii Rech. f. & Esfand. Journal of Evidence Based Complementary & Alternative Medicine 20(1): 65-72.
• Zolotar RM, Bykhovets AI, Kashkan ZN, Chernov YG, Kovganko NV (2002). Structure activity relationship of insecticidal steroids. VII. C-7-oxidized β-sitosterols and stigmasterols. Chemistry of Natural Compounds 38(2): 171-174.