Morpho-anatomical and phytochemical evaluation of Icacina trichantha Oliver (Icacinaceae)

Year 2020, , 100 - 105, 15.11.2020

Akeem Kadiri , Olayinka Asekun , Asekunow Anıta , Abiodun Ayodele , James Olowokudejo

https://doi.org/10.30616/ajb.774653

Abstract

The leaf epidermis of Icacina trichantha, a quintessential medicinal tropical plant was investigated with the aid of light microscopy and the chemical constituents of its leaf and root were investigated using Gas Chromatography-Mass Spectrometry (GC-MS). Hitherto, leaf epidermis data is missing and similarly, the chemical analysis of the leaf and root of the plant is undertaken in a single study for the first time. The leaf epidermis characters with which the species can be defined include paracytic and diacytic stomatal types, irregular epidermal cell shape together with angular or curved anticlinal wall patterns. However, the quantitative data appeared to overlap thus providing the range of values of both the measured and counted features. N-hexane extract of the root is rich in reducing sugars, tannins, steroids, glycosides, terpenoids and flavonoids while GC-MS analysis revealed 11 and 3 significant esterified bioactive components in the leaf and tuberous root respectively with dodecanoic acid being most abundantly present (47.85%-54.10 %) but some chemical confined to specific areas are trichothec-9-en-8-one (47.73 %) in the root and 9-octadecenoic acid (25.05 %) in the leaf. The result of this study will assist in identifying the plant even if its parts are fragmentary and also be helpful in screening the plant for drug.

Keywords

Microscopy, taxonomy, tropical plant

Supporting Institution

University of Lagos

Project Number

-

Thanks

Kadiri Akeem Babalola

Icacina trichantha Oliver’in (Icacinaceae) morfo-anatomik ve fitokimyasal değerlendirmesi

Abstract

Önemli bir tıbbi tropik bitki olan Icacina trichantha’nın yaprak epidermisi ışık mikroskobu yardımıyla, yaprağının ve kökünün kimyasal bileşenleri de Gaz Kromatografisi-Kütle Spektrometresi (GC-MS) kullanılarak incelenmiştir. Halihazırda bitkinin yaprak epidermis verileri eksik durumdadır ve bitkinin yaprağı ve kökünün kimyasal analizi tek bir çalışmada ilk kez yapılmıştır. Parasitik ve diasitik stoma tipleri, düzensiz epidermal hücre şekli ve köşeli veya kavisli antiklinal duvar desenleri türün tanımlanmasında kullanılabilen yaprak epidermis karakterleri araında yer alır. Bununla birlikte, nicel veriler, hem ölçülen hem de sayılan özelliklerin değer aralığını sağlıyacak şekilde örtüşür nitelikte görümektedir.Kökün N-heksan ekstresi indirgeyici şekerler, tanenler, steroidler, glikozitler, terpenoidler ve flavonoidler bakımından zengindir; GC-MS analizi de yaprak ve yumrulu kökte, en yaygını dodekanoik asit (%47.85 -%54.10) olan, sırasıyla 11 ve 3 önemli esterlenmiş biyoaktif bileşen ortaya çıkarmıştır. Buna karşın bazı kimyasalların belirli bölgelerle sınırlı (kökte trichothec-9-en-8-one, %47.73, yaprakta 9-oktadekenoik asit, %25.05) kaldığı görülmüştür. Çalışma bulguları, bitki bir bütün halinde olmasa bile bitkinin teşhisine katkı sağlayacaktır ve bitkinin drog olarak taranmasında da yardımcı olacaktır.

Keywords

Mikroskopi, Taksonomi, Tropik bitki

Project Number

-

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