The regulatory effects of resveratrol on the expression of renal MMP-2 and MMP-9 in the rat models of diabetes.

Abstract

Hyperglycemia caused by diabetes mellitus, in sensitive tissues such as the kidney, can cause dysfunction by damaging the blood vessels. Matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9, play a significant role in the degradation of proteins and angiogenesis; while epidermal growth factor receptor (EGFR) is involved in the recovery of damaged tissues. Herein, we investigated the relationship between MMP-2 and MMP-9 / EGFR in the kidney tissues of streptozotocin-induced Wistar male rats. We also examined the effects of resveratrol known as a strong tissue-protective on these changes. Diabetes was induced by streptozotocin (55 mg/kg) and trans-resveratrol (20 mg/kg/day intraperitoneal) was used for treatment. Rats were divided into 4 groups as control, resveratrol (Res), diabetes (Diab) and diabetes plus resveratrol (Diab+Res). Superoxide dismutase (SOD) and catalase (CAT) levels were significantly decreased in the kidneys in the diabetic group, whereas nitrite / nitrate, urea, creatine kinase (CK) and uric acid concentrations increased. MMP-2, MMP-9, calcium-binding protein B (S100B) and platelet-derived growth factor (PDGF) concentrations of kidney were increased although reduced EGFR in the diabetes group. Resveratrol supplementation markedly restored all these structures. Diabetes activates MMP related inflammation and oxidative stress by suppressing antioxidant enzymes in the kidney tissues. Resveratrol has partly modulatory effects on diabetes-induced changes.

Keywords

• Resveratrol
• Kidney
• MMP-2
• MMp-9
• Diabetes mellitus

Resveratrolün sıçan diyabet modellerinde renal MMP-2 ve MMP-9 ekspresyonu üzerindeki düzenleyici etkileri

Abstract

Diyabetes mellitusun neden olduğu hiperglisemi böbrek gibi hassas dokularda kan damarlarına zarar vererek işlev bozukluğuna neden olabilir. Matriks metaloproteinazlar (MMP'lar), özellikle MMP-2 ve MMP-9, proteinlerin bozunmasında ve anjiyojenezde önemli bir rol oynarken; epidermal büyüme faktörü reseptörü (EGFR) hasarlı dokuların iyileşmesinde rol oynar. Burada streptozotosin ile indüklenmiş Wistar erkek sıçanların böbrek dokularında MMP-2 ve MMP-9 / EGFR arasındaki ilişkiyi araştırdık. Ayrıca güçlü doku koruyucu olarak bilinen resveratrolün bu değişiklikler üzerindeki etkilerini inceledik. Diyabet modeli streptozotosin (55 mg / kg) ile oluşturuldu ve tedavi için trans-resveratrol (20 mg / kg / gün intraperitoneal) kullanıldı. Sıçanlar kontrol, resveratrol (Res), diyabet (Diab) ve diyabet + resveratrol (Diab + Res) şeklinde 4 gruba ayrıldı. Diyabet grubundaki sıçanların böbreklerinde superoksit dismutaz (SOD) ve katalaz (CAT) düzeylerinin önemli ölçüde azaldığı buna karşın nitrit / nitrat, üre, kreatinin kinaz (CK) ve ürik asit konsantrasyonlarının arttığı saptanmıştır. Diyabet grubunda renal EGFR düzeyinin azalmasına rağmen MMP-2, MMP-9, kalsiyum bağlayan protein B (S100B) ve trombosit kaynaklı büyüme faktörü (PDGF) düzeyleri artmıştır. Resveratrol takviyesi tüm değişkenleri önemli ölçüde iyileştirdi. Diyabet, böbrek dokularındaki antioksidan enzimleri baskılayarak MMP ile ilişkili inflamasyonu ve oksidatif stresi aktive eder. Resveratrol, diyabetin neden olduğu bu değişiklikler üzerinde kısmen modülatör etkilere sahiptir.

Keywords

• Diabetes mellitus
• resveratrol
• böbrek
• MMP-2
• MMP-9

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