Panax ginseng’in sıçanlarda sisplatin ile oluşturulan testis hasarı üzerine etkileri

Abstract

İnfertilite genetik, ailevi, hormonal nedenler, doğumsal anormallikler, çevresel ve kimyasal nedenlerle günümüzde artmakta olan önemli bir sağlık sorunu haline gelmiştir. Bu çalışma ile sıçanlarda sisplatin (CP) ile indüklenen testis hasarı üzerine Panax ginseng (Pnx) kök ekstraktının etkilerinin incelenmesi amaçlanmıştır. Sıçanlar Kontrol, Pnx (200 mg/kg), CP (7 mg/kg) ve CP+Pnx (200 mg/kg) olmak üzere 4 gruba ayrılmıştır. Deney sonunda sıçanların vücut ve testis ağırlıkları ölçülmüştür. Sıçanların kan örneklerinde serbest/total testosteron, toplam antioksidan kapasite (TAK) ve toplam oksidan durum (TOD) seviyeleri analiz edilirken, testis örneklerinde TUNEL boyaması ile apoptotik hücreler işaretlenmiştir. Sonuçlara göre hasar oluşturulmuş sıçanların plazma örneklerinde serbest/total testosteron ve TAK düzeyleri azalırken, TOD miktarı artmıştır. Testis dokularında seminifer tübül çapları genişlemiş ve apoptotik hücre sayısı artmıştır. Pnx uygulaması ile bu değişkenlerin ciddi oranda düzeldiği saptanmıştır. Sonuç olarak, Pnx’in testiküler dokuda belirgin koruyucu etkinliği bulunmaktadır; fakat etki mekanizmasının aydınlatılabilmesi için ileri çalışmalara gereksinim vardır.

Keywords

• Cisplatin
• Panax ginseng
• Apoptoz
• TAK
• TOD

Effects of Panax ginseng on cisplatin-induced testicular damage of rats

Abstract

Infertility has become an increasingly important health problem due to genetic, familial, hormonal, and congenital abnormalities, environmental and chemical reasons. This study aimed to investigate the effects of Panax ginseng (Pnx) root extract on cisplatin (CP) induced testicular damage of rats. Four animal groups were applied with different protocols as Control, Pnx (200 mg / kg), CP (7 mg / kg), and CP + Pnx (200 mg / kg). At the end of the experiment, the body and testicular weights of the rats were measured. While free/total testosterone, total antioxidant capacity (TAC), and total oxidative species (TOS) levels were analyzed in blood samples, apoptotic cells were marked by TUNEL staining in testicular samples of rats. According to the results, free/total testosterone and TAC levels were decreased while TOS levels increased in injured rats' plasma. On the other hand, seminiferous tubule diameters widened, and the number of apoptotic cells increased in rats' testis. These variables were significantly improved with the consumption of Pnx. As a result, Pnx has a significant protective effect on testicular tissue; however, further studies are needed to elucidate its action mechanism.

Keywords

• Apoptosis
• Cisplatin
• Panax ginseng
• TAC
• TOS

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