Year 2017, Volume 1 , Issue 1, Pages 4 - 8 2017-10-25

Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Muhammet DOGAN [1]


Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne, an aquatic plant belonging to the family Lythraceae, is used for the treatment of some diseases due to its medical and anti-microbial properties. This study presents multiple shoot regeneration from shoot tip and nodal explants of R. rotundifolia cultured on Murashige and Skoog (MS) nutrient medium containing 0.05-1.25 mg/L Kinetin (KIN) and 0.25 mg/L Gibberellic acid (GA3) combinations for eight weeks. At the end of the second week, shoot formations began to be observed on the explants. High shoot regeneration frequencies were determined for both explants in the culture medium. The maximum number of shoots per explant was obtained from shoot tip (38.66) and nodal (30.77) explants cultured on MS medium containing 0.25 mg/L KIN + 0.25 mg/L GA3. Whereas the minimum number of shoots per explant was determined on MS medium containing 1.25 mg/L KIN + 0.25 mg/L GA3 for both explant types.The highest shoot lengths for shoot tip (1.87 cm) and nodal (1.79 cm) explants were obtained on MS culture medium containing 0.75 mg/L KIN + 0.25 mg/L GA3 and 0.50 mg/L KIN + 0.25 mg/L GA3, respectively. For in vitro rooting of the regenerated shoots, 2 cm long cut shoots were transferred to MS medium containing 0.25 mg/L indole-3-butyric acid (IBA). The rooted shoots were then successfully acclimatized to external conditions in the aquarium environment. 
In vitro, Kinetin, R. rotundifolia, Shoot regeneration, Tissue culture
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Subjects Biology
Journal Section Articles
Authors

Author: Muhammet DOGAN
Country: Turkey


Dates

Publication Date : October 25, 2017

Bibtex @research article { ajb343304, journal = {Anatolian Journal of Botany}, issn = {}, eissn = {2602-2818}, address = {anatolianjbot@gmail.com}, publisher = {Abdullah KAYA}, year = {2017}, volume = {1}, pages = {4 - 8}, doi = {10.30616/ajb.343304}, title = {Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne}, key = {cite}, author = {Dogan, Muhammet} }
APA Dogan, M . (2017). Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne . Anatolian Journal of Botany , 1 (1) , 4-8 . DOI: 10.30616/ajb.343304
MLA Dogan, M . "Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne" . Anatolian Journal of Botany 1 (2017 ): 4-8 <https://dergipark.org.tr/en/pub/ajb/issue/31508/343304>
Chicago Dogan, M . "Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne". Anatolian Journal of Botany 1 (2017 ): 4-8
RIS TY - JOUR T1 - Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne AU - Muhammet Dogan Y1 - 2017 PY - 2017 N1 - doi: 10.30616/ajb.343304 DO - 10.30616/ajb.343304 T2 - Anatolian Journal of Botany JF - Journal JO - JOR SP - 4 EP - 8 VL - 1 IS - 1 SN - -2602-2818 M3 - doi: 10.30616/ajb.343304 UR - https://doi.org/10.30616/ajb.343304 Y2 - 2017 ER -
EndNote %0 Anatolian Journal of Botany Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne %A Muhammet Dogan %T Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne %D 2017 %J Anatolian Journal of Botany %P -2602-2818 %V 1 %N 1 %R doi: 10.30616/ajb.343304 %U 10.30616/ajb.343304
ISNAD Dogan, Muhammet . "Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne". Anatolian Journal of Botany 1 / 1 (October 2017): 4-8 . https://doi.org/10.30616/ajb.343304
AMA Dogan M . Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Ant J Bot. 2017; 1(1): 4-8.
Vancouver Dogan M . Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Anatolian Journal of Botany. 2017; 1(1): 4-8.
IEEE M. Dogan , "Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne", Anatolian Journal of Botany, vol. 1, no. 1, pp. 4-8, Oct. 2017, doi:10.30616/ajb.343304