Acorus calamus bitki ekstraktının prostat kanser hücre kültürü üzerine etkileri

Year 2018, Volume: 2 Issue: 1, 46 - 51, 01.05.2018

Halit Buğra Koca , Tülay Köken , Mete Özkurt Gökhan Kuş , Selda Kabadere Nilüfer Erkasap Oğuzhan Koca , Ömer Çolak

https://doi.org/10.30616/ajb.391985

Cited By: 5

Abstract

Batılı ülkelerde
erkekler arasında prostat kanseri en sık tanısı koulan kanser türüdür ve
erkeklerde kanser nedeniyle ölümde ikinci sırada yer almaktadır. Prostat
kanserinde vasküler endotelyal büyüme faktör-A’nın (VEGF-A) tek başına en
önemli anjiogenik faktör olduğu düşünülmektedir. Apoptotik süreçte yer alan
poli-(ADP-riboz) polimeraz’ın (PARP) yıkılımı apoptotik süreç markırı olarak
kullanılmaktadır. Acorus calamus’un
anti-kanserojen etkisi ile birlikte çeşitli tıbbi özelliklere sahip olduğu uzun
yıllardır özellikle Asya’da kabul edilmektedir. Çalışmamızda A. calamus kökünün etanolik ekstraktının
insan prostat kanser LNCaP hücre dizisinde hücre çoğalmasına, anjiogeneze ve
apoptozise olası etkileri in vitro
ortamda çalışıldı. Hücreler 24 ve 48 saat boyunca çeşitli ekstrakt
konsantrasyonlarıyla muamele edildi. Bu konsantrasyonlardaki ekstraktlar 24 ve
48 saatte LNCaP hücre yaşayabilirliğini kontrolle karşılaştırıldığında doza ve
zamana bağlı olarak %44’lere kadar azalttı. Bölünmüş PARP, VEGF-A proteini ve
gen ekspresyon miktarlarında 24 ve 48 saat sonra belirgin değişiklikler
gözlemlendi. Bu çalışma A.calamus
kökünün etanolik ekstraktının zaman ve doz bağımlı olarak güçlü bir
anti-kanser, anti-anjiogenik ve apoptotik etkileri olabileceğini ortaya
koymaktadır.

Keywords

LNCaP, Acorus calamus, anti-kanser, anti-anjiogenik, apoptotik

Effects of Acorus calamus plant extract on prostate cancer cell culture

Abstract

In western countries,
prostate cancer is the most frequently diagnosed cancer and the second most
common cause of death from cancer in men. Vascular endothelial growth factor-A
(VEGF-A), thought to be the single most important angiogenic factor in prostat
cancer. Poly-(ADP-ribose) polymerase (PARP) involved in apoptotic process and
cleavage of PARP serves as a marker of cells undergoing apoptosis. Acorus calamus have long been considered
to have anti-carcinogenic and medicinal properties especially in Asia. We
examined whether ethanolic extract of A.
calamus root affects the survival of prostate cancer LNCaP cells and
induces apoptosis and angiogenesis of these cells in vitro. Cells were incubated during 24 and 48 hours with various
doses of extract. Extract with these concentrations reduced the number of LNCaP
living cells up to 44 % as compared to the control at dose and time dependent
manner at 24 and 48 hours. Significantly alterations were observed at cleaved
PARP, VEGF-A protein and gene expression amounts after 24 and 48 hours. The
present study reveals the possibility that ethanolic extract of A. calamus root posseses a dose and time
dependent anticancer, apoptotic and anti-angiogenic properties.

Keywords

LNCaP, acorus calamus, anti-cancer, anti-angiogenic, apoptotic

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