Improving salt stress tolerance in Zea mays L. by modulating osmolytes accumulation and antioxidant capacity with Rutin

Year 2024, Volume: 8 Issue: 1, 21 - 29, 15.05.2024

Asiye Sezgin Muslu

https://doi.org/10.30616/ajb.1387695

Abstract

The growth and productivity of maize are severely affected by stress factors. Maize seedlings under salt stress were grown hydroponically to study the effect of rutin (Rut), a flavonoid, on changes in the stress parameters (thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O2), total chlorophyll), water status (leaf relative water content (RWC), osmolytes; proline, total soluble sugar), and activities of the main antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD)). After 21 days of growth, plants were applied with Rut as foliar spray. After 24 hours, seedlings were exposed to osmotic stress by 100 and 200 mM NaCI in the Hoagland’s Solution for 72 hours. Six groups were designed including a control (without NaCl or Rut), 150 mM NaCl, 200 mM NaCl, Rut, Rut+150 mM NaCl, and Rut+200 mM NaCl. Plant leaves were harvested 25 days after treatments. Exogenous significantly decreased TBARS and H2O2 contents in leaves of salt-stressed seedlings compared to salt stresses, enhanced the level of osmolytes, leaf RWC, activities of SOD, CAT, APX, and POD, and relative expression levels of SOD, CAT1, and APX1. As a result, findings from the study present reveal the effect of Rut on salt stress tolerance in maize seedlings under different osmotic stress. Here, it was clear that Rut played an active role in stress-alleviating. This application under salt stress can be useful in developing salt stress tolerance in crops for the agriculture sector.

Keywords

antioxidants, gene expression, osmolytes, rutin, salt stress

Rutin ile osmolit birikimi ve antioksidan kapasite modüle edilerek Zea mays L.'de tuz stresi toleransının geliştirilmesi

Abstract

Mısırın büyümesi ve verimliliği stres faktörlerinden ciddi şekilde etkilenir. Tuz stresi altındaki mısır fideleri, bir flavonoid olan rutinin (Rut) stres parametrelerindeki (tiyobarbitürik asit reaktif maddeleri (TBARS), hidrojen peroksit (H2O2), toplam klorofil), su durumu (yaprak nisbi su içeriği(NSİ), osmolitler; prolin, toplam çözünebilir şeker) ve ana antioksidan enzimlerin (süperoksit dismutaz (SOD), katalaz (CAT), askorbat peroksidaz (APX) ve peroksidaz (POD)) aktiviteleri üzerine etkisini incelemek için hidroponik olarak büyütüldü. 21 günlük büyümenin ardından yapraklara rutin sprey olarak gerçekleştirildi 24 saatin ardından fideler, 72 saat boyunca Hoagland Solüsyonunda 100 ve 200 mM NaCl'nin indüklediği ozmotik strese maruz bırakıldı. Deney grupları, Bir kontrol (NaCl veya Rut içermeyen), 150 mM NaCl, 200 mM NaCI, Rut, Rut+150 mM NaCl ve Rut+200 mM NaCl dahil olmak üzere üç tekrarlı altı grup şeklinde dizayn edildi. Bitki yaprakları uygulamalardan 25 gün sonra hasat edildi. Eksojen Rut, tuz stresine kıyasla tuz stresi altındaki fidelerin yapraklarındaki TBARS ve H2O2 içeriğini önemli ölçüde azalttı, osmolit seviyesini, yaprak NSİ’ni, SOD, CAT, APX ve POD aktivitelerini ve SOD, CAT1 ve APX1’in nisbi ekspresyon seviyelerini artırdı. Sonuç olarak, mevcut çalışmadan elde edilen bulgular, farklı ozmotik stres altındaki mısır fidelerinde Rut'un tuz stresi toleransı üzerindeki etkisini ortaya koymaktadır. Burada Rut'un stresi azaltmada aktif rol oynadığı açıktı. Tuz stresi altında yapılan bu uygulama, tarım sektörü için bitkilerde tuz stresine toleransın geliştirilmesinde faydalı olabilir.

Keywords

antioksidanlar, gen ifadesi, osmolitler, rutin, tuz stresi

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