Endemic Dactylorhiza osmanica subsp. osmanica: Ex vitro symbiotic germination approach

Year 2025, Volume: 9 Issue: 1, 51 - 54

Bengisu Şentürk , Erdi Can Aytar , Yasemin Özdener Kömpe

https://doi.org/10.30616/ajb.1570258

Abstract

In this study, the potential effects of Ceratobasidium sp. fungus on the ex vitro symbiotic germination of Dactylorhiza osmanica (Klinge) P. F. Hunt & Summerh subsp. osmanica seeds were evaluated. Results, Ceratobasidium sp. caused that about 51.95% germination rate was obtained in seeds inoculated. Protocorm and primordium formation rates were determined to be 25.27% and 26.67%, respectively. These findings indicate that the fungus promotes the germination of seeds. Ceratobasidium sp. on ex vitro symbiotic seed germination of D. osmanica subsp. osmanica shows the potential effect of the fungus Longer term and more detailed studies should be conducted to reach adult plants and adapt to natural conditions. These findings are very promising for protecting of endemic and rare orchid species.

Keywords

Ceratobasidium, conservation, endemic orchid, ex vitro symbiotic germination, mycorrhiza

Endemik Dactylorhiza osmanica subsp. osmanica: Ex vitro simbiyotik çimlenme için bir bakış açısı

Abstract

Bu çalışmada, Ceratobasidium sp. fungusunun, Dactylorhiza osmanica (Klinge) P. F. Hunt & Summerh subsp. osmanica tohumlarının ex vitro simbiyotik çimlenmesi üzerindeki potansiyel etkileri değerlendirilmiştir. Sonuçlar, Ceratobasidium sp. ile aşılanan tohumlarda yaklaşık %51,95 çimlenme oranı elde edildiğini göstermiştir. Protokorm ve primordiyum oluşum oranları sırasıyla %25,27 ve %26,67 olarak belirlenmiştir. Bu bulgular, fungusun tohum çimlenmesini teşvik ettiğini göstermektedir. Ceratobasidium sp.'nin D. osmanica subsp. osmanica üzerindeki ex vitro simbiyotik tohum çimlenmesi üzerindeki etkisi, fungusun potansiyel etkisini ortaya koymaktadır. Yetişkin bitkilere ulaşmak ve doğal koşullara adaptasyonu sağlamak için daha uzun süreli ve detaylı çalışmalar yapılması gerekmektedir. Bu bulgular, endemik ve nadir orkide türlerinin korunması için oldukça umut vericidir.

Keywords

Ceratobasidium, koruma, endemik orkide, ex vitro simbiyotik çimlenme, mikoriza

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