Background: Glutamate toxicity and reactive oxygen accumulation are life-threatening factors that play a role in the pathogenesis of neurodegenerative diseases. Today, many studies are carried out to elucidate these pathological pathways and to reveal possible treatment possibilities. Herbal medicines have been used continuously by people since ancient times due to their low side effects and easy accessibility. Glycyrrhiza glabra L. extract is also one of the plants often used for medicinal purposes. Since the antioxidant activity of Glycyrrhiza glabra L. has been reported in the studies, its protective activity at different doses was investigated in primary neuron culture with glutamate toxicity.
Methodology/Main findings: In the experiment, which consisted of control, Glutamate Toxicity, GG 100 µg/ml, GG 200 µg/ml and GG 400 µg/ml experimental groups, primary neuron culture was performed and some tests were applied in order to test cell viability and antioxidant activity in this culture.These tests are superoxide dismutase, total antioxidant level, total oxidant level, malondialhedit level, lactate dehydrogenase activity and MTT (3-(4,5-dimethyltriazol-2-yl)-2,5-diphenyltetrazolium bromide) tests. Our GG 200 µg/ml treatment group had the most significant results in all tests in statistical studies. In all tests, the glutamate toxicity group was compared with the Control group, and the treatment groups were statistically compared with the glutamate toxicity group.
Conclusion: Significant results were obtained in the tests applied to determine the oxidative stress and cell viability caused by glutamate toxicity. In the study, it was shown that Glycyrrhiza glabra L. significantly reduced glutamate-induced cell death, LDH release, lipid peroxidation and oxidation in primary neuronal cells.