Effect of toluene addition on pyocyanin production in the presence of different carbon sources

Year 2022, Volume: 3 Issue: 1, 25 - 27, 30.06.2022

Sümeyra Gürkök , Murat Özdal

Abstract

Pseudomonas aeruginosa is a Gram-negative, opportunistic bacterium and is one of the most biotechnologically important microorganisms. P. aeruginosa secretes an extracellular secondary metabolite known as pyocyanin, which is highly functional. In this study, the effects of toluene on pyocyanin production in different carbon sources in submerged culture of P. aeruginosa OG1 were investigated. It was determined that the addition of toluene to all carbon sources increased the production of pyocyanin. Maximum pyocyanin production was achieved when glycerol was used as the sole carbon source. With the addition of toluene, pyocyanin production generally increased, but bacterial biomass decreased. In addition, when glucose was used as the carbon source, the final pH decreased more than the other carbon sources. This study revealed that the addition of toluene to the fermentation medium significantly increased the production of pyocyanin in the presence of different carbon sources. These findings support that the solvent assisted fermentation strategy can be used in microbial fermentations to increase the production of industrially important biotechnological products such as pigments.

Keywords

Pseudomonas aeruginosa, pyocyanin, tolune, carbon sources

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