The effects of priming applications with chitosan, seaweed and humic acid on germination in stored Anatolian Black Pine seeds

Abstract

Background and aims Even if forest tree seeds are stored under convenient temperature and humidity conditions, they getting age over time. Especially physiological seed quality decreases due to aging. In this context; Priming, as a pre-process, is one of the most common practices used to improve seed quality. Methods In this study; Anatolian Black Pine (Pinus nigra Arnold. subsp. pallasiana Lamb. (Holmboe)) seeds, harvested from the Bursa-İnegöl-Boğazova clonal seed orchard with the national code TB-82 in 2006 and 2018 and stored thereafter, were used. In seed selection; Anatolian Black Pine seeds, which are in the orthodox category and widely used in afforestation studies, were preferred. Therefore, priming with materials such as chitosan, seaweed, and humic acid has been applied to improve the low germination capacity of Anatolian Black Pine seeds stored for long-term. In the study; The effects of priming materials and doses on seed germination were investigated. Results It was determined that chitosan application (0.5% and 0.75%) had a statistically significant effect on germination rate and percentage compared to other priming materials and doses. It has also been found that seaweed application significantly reduces average germination time. Conclusions It has been determined that chitosan application significantly increases the germination rate and percentage, especially in old seeds. With this; Further research is recommended to determine the effects of priming processes on Anatolian black pine and other forest tree seeds whose viability and germination ability have decreased.

Keywords

• Black pine
• priming
• long-term storaged
• aging

Project Number

KU-BAP01/2021-21 ve KU-HIZDES/2021-06

Depolanmış Anadolu Karaçamı tohumlarına kitosan, deniz yosunu ve hümik asit ile priming uygulamanın çimlenme üzerine etkileri

Abstract

Giriş ve Hedefler Orman ağacı tohumları her ne kadar uygun sıcaklık ve nem koşullarında depolansalar dahi zamana bağlı olarak yaşlanmaktadırlar. Yaşlanmaya bağlı olarak özellikle fizyolojik tohum kalitesi düşmektedir. Bu bağlamda; Priming, bir ön işlem olarak, tohum kalitesini arttırmada kullanılan en yaygın uygulamalardan biridir. Yöntem Bu çalışmada; TB-82 ulusal kodlu Bursa-İnegöl-Boğazova klonal tohum bahçesinden 2006 ve 2018 yıllarında hasat edilen ve depolanan Anadolu Karaçamı (Pinus nigra Arnold. subsp. pallasiana Lamb. (Holmboe)) tohumları kullanılmıştır. Tohum seçiminde ortodoks kategorisinde yer alan ve ağaçlandırma çalışmalarında yaygın olarak kullanılan karaçam tohumları tercih edilmiştir. Dolayısı ile uzun süre depolanan Anadolu karaçam tohumlarında meydana gelen çimlenme kapasitesi düşüklüğünün iyileştirilmesinde kitosan, deniz yosunu ve hümik asit gibi materyaller ile priming uygulanmıştır. Çalışmada; priming materyallerinin ve dozlarının tohumların çimlenmesi üzerindeki etkisi araştırılmıştır. Bulgular Çimlenme hızı ve yüzdesi üzerine kitosan uygulamasının (%0,5 ve %0,75) diğer priming materyalleri ve dozlarına göre istatistiksel olarak anlamlı etki yaptığı tespit edilmiştir. Ayrıca, deniz yosunu uygulamasının ortalama çimlenme süresini önemli ölçüde azalttığı da tespit edilmiştir. Sonuçlar Kitosan uygulamasının bilhassa yaşlı tohumlarda çimlenme hızı ve yüzdesini önemli oranda artırdığı tespit edilmiştir. Bununla birlikte; canlılığı ve çimlenme kabiliyeti düşmüş Anadolu karaçamı ve diğer orman ağacı tohumları üzerine priming işlemlerinin etkilerini belirlemeye yönelik ileri araştırmalar önerilmektedir.

Keywords

• Karaçam
• Priming
• Uzun süre depolama
• yaşlanma

Project Number

KU-BAP01/2021-21 ve KU-HIZDES/2021-06

Thanks

Şeyma Selin Akın, Kastamonu Üniversitesi Fen Bilimleri Enstitüsü Sürdürülebilir Ormancılık Programında Yükseköğretim Kurulu'nun 100/2000 bursuyla ve TÜBİTAK 2211/A yurtiçi genel doktora bursu ile desteklenmiştir. Ayrıca, araştırma materyali Anadolu karaçamı tohumlarının tedarikini sağlayan Bursa Orman Fidanlık Müdürlüğüne teşekkür ederiz.

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