A Study on Adventitious Shoot Formation from Stem and Petiole Explants of Grape (Vitis vinifera L.)

Year 2008, Volume: 21 Issue: 2, 231 - 240, 01.12.2008

Z. Babalık N. Göktürk Baydar

Abstract

In this study, the effects of explant type, nutrient media and culture conditions on in vitro regeneration of stem and petiole explants taken from in vitro plants of Kalecik karası were investigated. The explants were planted on MS and NN medium supplemented with different concentrations of BAP, 2,4-D, zeatin, IBA and casein hydrolisate and then they were cultured in dark or light conditions. As a result of this research, formation of callus, direct and indirect adventitious shoot formation were obtained in different ratio according to explant type, nutrient media and culture conditions. Stem explants gave the best results for the direct and indirect adventitious shoot formation. While formation of callus were obtained from MS medium added 0.2 mg/l BAP+1 mg/l 2,4-D+2.5 mg/l IBA and NN medium added 2 mg/l 2,4-D the highest direct adventitious shoot formation were obtained from MS medium added 2 mg/l zeatin and NN medium added 2 mg/l BAP and indirect adventitious shoot formation were obtained from MS medium added 0.2 mg/l BAP+1 mg/l 2,4-D+1 g/l casein hydrolisate and NN medium contained 2 mg/l zeatin. Dark condition was showed positive effect on formation of callus. On the other hand, 16/8 h light regime increased direct and indirect adventitious shoot formation.

Keywords

Grapevine (Vitis vinifera L.), explant type, nutrient media, culture conditions, in vitro regeneration

ASMADA (Vitis vinifera L.) GÖVDE VE YAPRAK SAPI EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU ÜZERİNE BİR ARAŞTIRMA

Abstract

Bu araştırmada bitkisel materyal olarak Kalecik karası üzüm çeşidine ait in vitro bitkilerden alınan gövde ve yaprak sapı eksplantlarının in vitro rejenerasyonları üzerine eksplant tipi, besin ortamları ile ışıklanma süresinin etkileri incelenmiştir. Eksplantlar, farklı konsantrasyonlarda BAP, 2,4-D, zeatin, IBA ve kazein hidrolizat içeren MS ve NN ortamlarına dikildikten sonra aydınlık ve karanlık koşullarda kültüre alınmışlardır. Araştırma sonucunda kallus oluşturan eksplant oranı, direkt ve indirekt adventif sürgün oluşumu, adventif sürgünlerin bitkiye dönüşüm oranı eksplant tipine, besin ortamlarına ve ışıklanma süresine göre değişmiştir. Buna göre gövde eksplantları direkt ve indirekt adventif sürgün oluşumu bakımından en iyi sonuçları verirken; ortamlar içinde kallus oluşumu en fazla 0,2 mg/l BAP+1 mg/l 2,4-D+2,5 mg/l IBA içeren MS ve yalnızca 2 mg/l 2,4-D içeren NN ortamlarından, direkt adventif sürgün gelişimi de 2 mg/l zeatin içeren MS ortamı ile 2 mg/l BAP içeren NN ortamında, indirekt adventif sürgün gelişimi ise 0,2 mg/l BAP+1 mg/l 2,4-D+1 g/l kazein hidrolizat katkılı MS ortamı ile 2 mg/l zeatin NN ortamından elde edildiği belirlenmiştir. Karanlık kültür koşulları kallus oluşumu üzerinde olumlu etkiler de bulunurken; 16/8 sa ışıklanma ise direkt-indirekt adventif sürgün gelişimini artırdığı tespit edilmiştir

Keywords

Asma (Vitis vinifera L.), eksplant tipi, besin ortamı, kültür koşulları, in vitro rejenerasyon

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