Fiber Development Characteristics of Cotton Mutants: fuzzless-lintless, Ligon lintless and fuzzless-lint

Year 2004, Volume: 17 Issue: 1, 95 - 99, 01.06.2004

M. Karaca

Abstract

The main objective of this research was to compare fiber initiation properties of 3 different cotton mutants; fuzzless-lintless (fl), recessive mutant which does not produce fuzz and lint fibers, Ligon lintless (Li1), a monogenic novel dominant mutant producing only short fibers (short fuzz and lint fibers) and fuzzless-lint (f-142) which only produces lint fibers. In this research, a Scanning Electron Microscopy (SEM) was used to monitor the changes in the fiber initiations. Studies indicated that the fiber initiation of Li1 and f-142 at 0, 1 and 3 days post anthesis (DPA) were very similar with that of the normal cotton, Texas Marker 1, (TM1). However, at later stages fiber elongation stopped in the Li1 ovules at about 8-10 DPA, whereas it continued in the TM1 and f-142 ovules. The fuzzless-lintless ovules failed to produce both fuzz and lint fibers. The use of fuzzless-lint, fuzzless-lintless and Li1 mutants clearly indicated that fuzz fibers and lint fibers initiate at the same time but they are controlled by different genes. Present study also suggested that Li1 gene is active during the latter stages of fiber development, probably during the later elongation phase.

Keywords

fiber, cotton, SEM, fuzz and lint fibers

Pamuk Lif Mutantlarının Gelişim Özellikleri: lintersiz-lintsiz, Ligon lintsiz ve lintersiz-lint

Abstract

Bu çalışmanın ana amacı üç farklı mutant pamuğun; linter (hav) ve lint üretmeyen resesif mutant lintersiz-lintsiz (fl), kısa lifler veren ve tek bir gen tarafından kontrol edilen, seçkin bir dominant Ligon lintless (Li1) ve yalnızca lint üreten lintersiz fuzzless-lint (f-142) lif oluşumunu başlatma özelliklerinin karşılaştırılmasıdır. Çalışmada bir Tarama Elektron Miksroskop (TEM) kullanılarak lif oluşumundaki değişiklikler izlenmiştir. Sonuçlar Li1 ve f-142’nın çiçeklenmeden 0, 1 ve 3 gün sonraki lif gelişiminin normal Teksas Markır (TM-1) pamuğuna çok benzediğini göstermiştir. Ancak ileriki dönemlerde lif uzaması mutant ovüllerinde çiçeklenmeden 8-10 gün sonra dururken normal TM1 ve mutant fuzzless-lint’de devam etmiştir. Lintersiz-lintsiz mutant ovüllerinde ise linter yada lint gelişememiştir. Lintersiz-lintsiz, lintersiz-lint ve Li1 mutantlarının kullanılması açıkça göstermiştir ki linter ve lint lifleri aynı zamanda gelişmeye başlamakta ancak bunlar farklı genler tarafından kontrol edilmektedir. Bu çalışma yine mutant Li1, geninin ileriki dönemlerinde, tahminen ileri lif uzama döneminde aktif olduğunu göstermiştir.

Keywords

Lif, Pamuk, TEM, Linter ve Lint Lifleri

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