Manda sütünde doğrudan mikroskobik somatik hücre sayısının belirlenmesinde sulandırma oranının etkisi
Abstract
The objective of this study was to investigate the accurate dilution ratio for determination of somatic cell count (SCC) in buffalo milk by direct microscopic method. Milk samples taken from four Anatolian water buffalo raising farms of two towns of Turkey were diluted with distilled water at 1:1, 1:2, 1:4 and 1:6 dilution ratio (RD) and tested by SCC using direct microscopic method. While 1:1 and 1:2 RD were different from 1:4 and 1:6 RD (P< 0.05), SCC groups were not different by towns and farms. Correlation coefficient (r= -0.481) between SCC and RD was estimated to be significant (P< 0.05). The results reveal that water buffalo milk samples should not be diluted higher than 1: 2 RD for obtaining true SCC data.
References
- Atasever S, Erdem H (2008) Water buffalo raisıng and its future in Turkey. Anadolu J. Agric. Sci,. 23(1):59-64.
- Atasever S, Erdem H, Kul H (2011) Relationship between somatic cell count and catalase activity in raw milk of Anatolian buffaloes. Sci.Res. Essays, 6(19): 4109-4112.
- Atasever, S (2012) Estimation of correlation between somatic cell count and coagulation score of bovine milk. Int. J. Agric. Biol., 14:315-317.
- Cerón-Muñoz M, Tonhati H, Duarte J, Oliveira J, Munoz-Berrocal M, Jurado-Gamez H (2002) Factors affecting somatic cell counts and their relations with milk and milk constituent yield in buffaloes. J. Dairy Sci,. 85: 2885–2889.
- Khosroshahi ZT, Rafat SA, Shoja D (2011) Effects of non-genetic factors in milk production and composition in East Azarbaijan native buffaloes of Iran. Buffalo Bull., 30(3): 202-209.
- Ogola H, Shitandi A, Nanua J (2007) Effect of mastitis on raw milk compositional quality. J.Vet.Sci., 8(3): 237-242.
- Ozenci E, Vural MR, Seker E, Ucar M (2008) An evaluation of subclinical mastitis during lactation in Anatolian Buffaloes. Turk. J. Vet. Anim. Sci., 32(5): 359-368.
- Packard VS, Tatini JrS, Fugua R, Heady J, Gilman C (1992) Direct Microscopic Methods for Bacteria or Somatic Cells. In: Standard Methods for the Examination of Dairy Products, Marshall, R.T. (Ed.). 16th Edn., American Public Health Association, Washington, DC, USA., pp: 309-325.
- Sekerden O (2011) Factors affecting somatic cell counts and their relations with milk and milk constituent yield in Anatolian and F1 Anatolian x Italian crossbred buffaloes. J. Anim. Prod., 52(1): 9-16.
- Sharma N, Singh NK, Bhadwal MS (2011) Relationship of somatic cell count and mastitis: An overview. Asian-Aust. J. Anim. Sci., 24(3): 429-438.
- Syed AM, Digraskar SU, Awaz KB (2009) Evaluation of buffalo milk with reference to somatic cell count and antitrypsin. Vet. World, 2(7):267-268.
Effect of dilution ratio on determination of somatic cell count in buffalo milk by direct microscopy
Abstract
The objective of this study was to investigate the accurate dilution ratio for determination of somatic cell count (SCC) in buffalo milk by direct microscopic method. Milk samples taken from four Anatolian water buffalo raising farms of two towns of Turkey were diluted with distilled water at 1:1, 1:2, 1:4 and 1:6 dilution ratio (RD) and tested by SCC using direct microscopic method. While 1:1 and 1:2 RD were different from 1:4 and 1:6 RD (P< 0.05), SCC groups were not different by towns and farms. Correlation coefficient (r= -0.481) between SCC and RD was estimated to be significant (P< 0.05). The results reveal that water buffalo milk samples should not be diluted higher than 1: 2 RD for obtaining true SCC data.
References
- Atasever S, Erdem H (2008) Water buffalo raisıng and its future in Turkey. Anadolu J. Agric. Sci,. 23(1):59-64.
- Atasever S, Erdem H, Kul H (2011) Relationship between somatic cell count and catalase activity in raw milk of Anatolian buffaloes. Sci.Res. Essays, 6(19): 4109-4112.
- Atasever, S (2012) Estimation of correlation between somatic cell count and coagulation score of bovine milk. Int. J. Agric. Biol., 14:315-317.
- Cerón-Muñoz M, Tonhati H, Duarte J, Oliveira J, Munoz-Berrocal M, Jurado-Gamez H (2002) Factors affecting somatic cell counts and their relations with milk and milk constituent yield in buffaloes. J. Dairy Sci,. 85: 2885–2889.
- Khosroshahi ZT, Rafat SA, Shoja D (2011) Effects of non-genetic factors in milk production and composition in East Azarbaijan native buffaloes of Iran. Buffalo Bull., 30(3): 202-209.
- Ogola H, Shitandi A, Nanua J (2007) Effect of mastitis on raw milk compositional quality. J.Vet.Sci., 8(3): 237-242.
- Ozenci E, Vural MR, Seker E, Ucar M (2008) An evaluation of subclinical mastitis during lactation in Anatolian Buffaloes. Turk. J. Vet. Anim. Sci., 32(5): 359-368.
- Packard VS, Tatini JrS, Fugua R, Heady J, Gilman C (1992) Direct Microscopic Methods for Bacteria or Somatic Cells. In: Standard Methods for the Examination of Dairy Products, Marshall, R.T. (Ed.). 16th Edn., American Public Health Association, Washington, DC, USA., pp: 309-325.
- Sekerden O (2011) Factors affecting somatic cell counts and their relations with milk and milk constituent yield in Anatolian and F1 Anatolian x Italian crossbred buffaloes. J. Anim. Prod., 52(1): 9-16.
- Sharma N, Singh NK, Bhadwal MS (2011) Relationship of somatic cell count and mastitis: An overview. Asian-Aust. J. Anim. Sci., 24(3): 429-438.
- Syed AM, Digraskar SU, Awaz KB (2009) Evaluation of buffalo milk with reference to somatic cell count and antitrypsin. Vet. World, 2(7):267-268.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 1, 2014 |
| Published in Issue | Year 2014 Volume: 27 Issue: 2 |