Identification of Five Allium Species with RAPD Markers

Leyla Açık; Bülent Samancı; Mehmet Yapar; Ayhan Kubar

Research Article

Identification of Five Allium Species with RAPD Markers

Year 1997, Volume: 10 Issue: 1, 143 - 148, 01.12.1997

Leyla Açık Bülent Samancı Mehmet Yapar Ayhan Kubar

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to Allium species in order to check the degree of polymorphism within the genus. Five species of Allium, A. isauricum, A. myrianthum, A. curtum, A. ılgazasense and A. kararnanoglu were evaluated for variability using a set 10mer random primers. One of the primer revealed scorable polymorphism between Allium species. Variation in banding profiles between species were observed. These were applied to systematic studies within the genus. Ten band positions were scored. Genetic distances between species were calculated and cluster analysis were used for a dendogram showing phylogenetic relations among species studied.

Keywords

RAPD-PCR, Allium, Allium Taxonomy, Phylogeny

References

1. AÇIK, L., B. SAMANCI, H. DUMAN, F. ÜNAL. Polymorphism and Phlylogentic Relations Among Turkish Species In the genus Stenbergia as Determined RAPD-PCR. Turkish Jouna! of Botany. In press.
2. DAVIES, P.H. In Flora of Turkey and East Agean Islands, vol VIII. pp. 360- 364, 1984.
3. HANELT, P. In Onions and Applied Crops. Botany, Physiology and Genetics. CRC Press Inc. Boca, Raton, Florida pp. 1- 6, 1990.
4. LANDRY, B.S., R.Q. LI, W.Y. CHEUNG, R.L. GARANGER. Phlogeny Analysis of 25 Apple Rootstocks Using RAPD Markers and Tactical Gene Tagging. Theor. Appl. Genet. 89, 847-852, 1994
5. SAMBROOK, J. F.F. FRITCH, T. MANÎATIS. In Molecular Clonning. A laboratory Manual,. Second edition. Cold Spring Harbour. 1989.
6. WELSH, J. C. PETERSON, M. MCCLLELAND. Polymorphism Generated by Arbitrarily Primed PCR in The mouse; Applications to Strain Identification and Genetic Mapping. Nucleic Acids Res. 19, 903-906, 1991.
7. WELSH, J., C. PRETZMEN, D. POSTIC, T. SAINT-GIRONS, G. BATONTON, M, MCCLELLAND. Genomic Fingerprinting by Arbitrarily Primed PCR Resolves Borrelia burgdorferi into Three Distinct Phyletic Groups. Int. Sys. Bacterid, 42, 370-377, 1992.
8. WILKIE, S.E., P.G. ISAAC, AND J. R. SLATER. RAPD Markers for Genetic Analysis in Allium. Theor. Appl. Genet. 86,497-504, 1993.
9. WILLIAMS, J.G., A.R. KUBELIK, K. LLIVAC, J. A. RAFALSKI, S. TINGEY. DNA Polymorphism Amplified by Arbitrary Primers Are Useful as Genetic Markers. Nucleic Acids Research. 22, 6531-6535, 1990.
10. WOLF, K, J.P. RIJN. Rapid Detection of Genetic Variabilty in Chrisanthemun Using Random Primers. Heridity. 71,335-341, 1993.

RAPD İzleri ile 5 Allium Türünün Belirlenmesi

Year 1997, Volume: 10 Issue: 1, 143 - 148, 01.12.1997

Leyla Açık Bülent Samancı Mehmet Yapar Ayhan Kubar

Abstract

RAPD analizi cins içersinde polimorfizmim derecesini kontrol etmek için Allium türlerine uygulanmıştır. Allium 'un beş türü, A. isauricum, A. myrianthum, A. curtum, A. ılgazasense and A. kararnanoglu şansa bağlı primerler kullanılarak vary asyonu belirlemek için değerlendirilmişlerdir. Primerlerden biri Allium türleri arasında sayılabilir polimorfizmi ortaya koymuştur. Band profilinde türler arasındaki varyasyonlar gözlenmiştir. Bunlar cins içersinde sistematik çalışmalara uygulanmıştır. 10 band pozisyonu sayılmıştır. Türler arasındaki genetik uzaklıklar hesaplanmış ve kluster analizi çalışılan türler arasındaki filogenetik ilişkileri gösteren dendogram için kullanılmıştır.

Keywords

RAPD-PCR, Allium, Soğan Taxonomisi, Filojeni

References

1. AÇIK, L., B. SAMANCI, H. DUMAN, F. ÜNAL. Polymorphism and Phlylogentic Relations Among Turkish Species In the genus Stenbergia as Determined RAPD-PCR. Turkish Jouna! of Botany. In press.
2. DAVIES, P.H. In Flora of Turkey and East Agean Islands, vol VIII. pp. 360- 364, 1984.
3. HANELT, P. In Onions and Applied Crops. Botany, Physiology and Genetics. CRC Press Inc. Boca, Raton, Florida pp. 1- 6, 1990.
4. LANDRY, B.S., R.Q. LI, W.Y. CHEUNG, R.L. GARANGER. Phlogeny Analysis of 25 Apple Rootstocks Using RAPD Markers and Tactical Gene Tagging. Theor. Appl. Genet. 89, 847-852, 1994
5. SAMBROOK, J. F.F. FRITCH, T. MANÎATIS. In Molecular Clonning. A laboratory Manual,. Second edition. Cold Spring Harbour. 1989.
6. WELSH, J. C. PETERSON, M. MCCLLELAND. Polymorphism Generated by Arbitrarily Primed PCR in The mouse; Applications to Strain Identification and Genetic Mapping. Nucleic Acids Res. 19, 903-906, 1991.
7. WELSH, J., C. PRETZMEN, D. POSTIC, T. SAINT-GIRONS, G. BATONTON, M, MCCLELLAND. Genomic Fingerprinting by Arbitrarily Primed PCR Resolves Borrelia burgdorferi into Three Distinct Phyletic Groups. Int. Sys. Bacterid, 42, 370-377, 1992.
8. WILKIE, S.E., P.G. ISAAC, AND J. R. SLATER. RAPD Markers for Genetic Analysis in Allium. Theor. Appl. Genet. 86,497-504, 1993.
9. WILLIAMS, J.G., A.R. KUBELIK, K. LLIVAC, J. A. RAFALSKI, S. TINGEY. DNA Polymorphism Amplified by Arbitrary Primers Are Useful as Genetic Markers. Nucleic Acids Research. 22, 6531-6535, 1990.
10. WOLF, K, J.P. RIJN. Rapid Detection of Genetic Variabilty in Chrisanthemun Using Random Primers. Heridity. 71,335-341, 1993.

There are 10 citations in total.

Details

Primary Language	English
Subjects	Agricultural Engineering
Journal Section	Articles
Authors	Leyla Açık This is me Bülent Samancı This is me Mehmet Yapar This is me Ayhan Kubar This is me
Publication Date	December 1, 1997
Published in Issue	Year 1997 Volume: 10 Issue: 1

Cite

APA	Açık, L., Samancı, B., Yapar, M., Kubar, A. (1997). Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture, 10(1), 143-148.
AMA	Açık L, Samancı B, Yapar M, Kubar A. Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture. December 1997;10(1):143-148.
Chicago	Açık, Leyla, Bülent Samancı, Mehmet Yapar, and Ayhan Kubar. “Identification of Five Allium Species With RAPD Markers”. Akdeniz University Journal of the Faculty of Agriculture 10, no. 1 (December 1997): 143-48.
EndNote	Açık L, Samancı B, Yapar M, Kubar A (December 1, 1997) Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture 10 1 143–148.
IEEE	L. Açık, B. Samancı, M. Yapar, and A. Kubar, “Identification of Five Allium Species with RAPD Markers”, Akdeniz University Journal of the Faculty of Agriculture, vol. 10, no. 1, pp. 143–148, 1997.
ISNAD	Açık, Leyla et al. “Identification of Five Allium Species With RAPD Markers”. Akdeniz University Journal of the Faculty of Agriculture 10/1 (December 1997), 143-148.
JAMA	Açık L, Samancı B, Yapar M, Kubar A. Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture. 1997;10:143–148.
MLA	Açık, Leyla et al. “Identification of Five Allium Species With RAPD Markers”. Akdeniz University Journal of the Faculty of Agriculture, vol. 10, no. 1, 1997, pp. 143-8.
Vancouver	Açık L, Samancı B, Yapar M, Kubar A. Identification of Five Allium Species with RAPD Markers. Akdeniz University Journal of the Faculty of Agriculture. 1997;10(1):143-8.