Sekonder Metabolitlerin Üretilmesinde Biyoteknolojik Çalışmaların Kullanılması

Year 1997, Volume: 10 Issue: 1, 391 - 400, 01.12.1997

Gülhan Ercan Kenan Turgut

Abstract

Sınırsız doğal ürün kaynağına sahip olan bitkilerde, şimdiye kadar bitki hücre kültürleri ve transforme edilmiş kök kültürlerinden 140 doğal ürünün varlığı bildirilmiştir. Özellikle sekonder
metabolitlere antimikrobiyal, anribiyotik, insektisit ve hormonal özellikleri ile etkili biyolojik ve farmakolojik aktivitelerinden dolayı büyük ilgi vardır.
Bu derlemede sekonder metabolitlerin, biyoteknolojik olarak üretimi için bitki hücre ve doku kültürü potansiyellerini gösteren bazı sonuçlar verilmiştir.

Keywords

Sekonder Metabolit, Hücre Kültürü, Agrobacterium rhizogenes

The Use of Biotechnology for Producing of Secondary Metabolites

Abstract

Plants represent unlimited source of natural products. Up to now 140 natural products have been known from plant cell cultures and transformed root cultures. Especially the secondary compounds are major interest because of their impressive biological activities ranging from antimicrobiai, antibiotic. Insecticidal, hormonal proporties to highly important pharmacological activities.
In this rewiev, some results which show the potential of plant cell and tissue cultures for a biotechnological production of secondary metabolites are presented.

Keywords

Secondary Metabolites, Cell Culture, Agrobacterium rhizogenes

References

• 1. ALFERMANN, A.W., PETERSEN M., Natural Product Formation by Plant Cell Biotechnology, Plant Cell, Tissue and Organ Culture, 43, 199- 205, 1995.
• 2. AKMAN, Y., Botanik, Bitki Biyolojisine Giriş, Ankara, 1989.
• 3. BAUMERT, A., GRÖGER D., KUZOUKAMA IN. and REISCH J., Secondary Metabolites Produced by Callus Cultures of Various Ruta Species, Plant Cell, Tissue and Organ Culture, 28, 159-162, 1992.
• 4. BOHLMANN, J., EILERT U., Elicitor Induced Secondary Metabolism in Ruta graveolens L. Plant Cell, Tissue and Organ Culture, 38, 189-198, 1994.
• 5. ERCAN, G., S. YÜCE, K. TURGUT, Kökboya Bitkisinin (.Rubia tinctorum L.) İn-vitro koşullarda Rejenerasyon Yeteneğinin Araştırılması, Turkish Journal of Agriculture and Forestry, 21, 487- 491, 1997.
• 6. FURMANOWA, M. and OLSZOWSKA O., Micropropagation of Thyme (Thyme vulgaris L.), Biotechnology in Agriculture and Forestry, 19, 230-242, 1992.
• 7. GIROD, P.A. ZRYD J.P., Secondary Metabolism in Cultured Redbeet (Beta vulgaris L.) Cells: Differential Regulation of Betaxanthin and Betacyanin Biosynthesis, Plant Cell, Tissue and Organ Culture, 25, 1-12, 1991.
• 8. GÖNÜLŞEN, N., Bitki Doku Kültürleri Yöntemleri ve Uygulama Alanları, Tarım Orman ve Köyişleri Bakanlığı Ege Tarımsal Araştırma Enstitüsü Müdürlüğü, Yayın no. 78,1987.
• 9. HAGENDORN, M.J.M, PLAS L.H.W., SEGERS G.J., Accumalation of Anthraquinones in Morinda citrifolia Cell Suspensions, Plant Cell, Tissue and Organ Culture, 38, 227-234, 1994.
• 10. HEIJDEN, R., VERPOORTE R., HOEKSTRA S.S., and HOGE H.C., Nordamnacanthal, a Major Anthraquinone from on Agrobacterium rhizogenes induced Root Culture of Rubia tinctorum, Plant Physiol, Biochem, 32(3), 399- 404, 1994.
• 11. McAfee, B.J., WHITE E.E., PELCHER L.E. and LAPP M.S., Root Induction in Pine (Pinus) and Larch (Larix) spp. Using Agrobacterium rhizogenes, Plant Cell, Tissue and Organ Culture, 34, 53-62, 1993.
• 12. OGASAWARA, T., CHIBA K. and TADA M., Production in High-yield of Naphtoquinone by a hairy-root culture of Sesamum indicum, Phytochemistry, 33(5), 1095-1098, 1993.
• 13. POULSEN, C., High-performance Liquid Chromatographic Assay of Anthranilate synthase from Plant Cell Cultures, Journal of Chromatography, 547,155-160, 1991.
• 14. RHODES, M.J.C., PARR A.J., GIULIETTI A. and AIRD E.L.H., 1992. Influence of Exogenous Hormones on the Growth and Secondary Metabolite Formation in Transformed Root Cultures, Plant Cell, Tissue and Organ Culture, 38, 143-151.
• 15. ROUTIEN, J.B., NICKEL L.G., Cultivation of Plant Tissue. US Patent 2, 747, 334, 1952.
• 16. SAKAMOTO, K., ADA K., SAWAMURA K., HAJIRO K., ASADA Y., YOSHIKAWA T., FURUYA T, Anthociyanin Production in Cultured Cells of Aralia cor data Thunb., Plant Cell, Tissue and Organ Culture, 36, 21- 26, 1994.
• 17. SATO, K., YAMAZAKI T., OKUYAMA E., YOSKH3RA K., SHBVÎOMURA K., Antraquinone Production by Transformed Root Cultures of Rubia tinctorum: Influence of Phytohormones and Sucrose Concentration, Phytochemistry, 30(5), 1507-1509, 1991.
• 18. SATO, K., GODA Y., KAWASAKI Y., OKUYAMA E., YOSHIHIRA K., NAKAMURA M., Characteristic of Anthraquinone Production in Plant Roots and Cell Suspension Cultures of Rubia Tinctorum and Rubia akane, Plant Tissue Culture Letters, 9(3), 1992.
• 19. SIERRA, M.I., HEIJDEN R, LEER T. and VERPOORTE R., Stability of Alkoloid Production in Cell Suspancions Cultures of Tabernaemontana divericata during Long-term Subculture, Plant Cell, Tissue and Organ Culture, 28, 59- 68,1992.
• 20. VAGNEROWA, H., Micropropagation of Common Centaury (Centaurium erythraea Rafh.), Biotechnology in Agriculture and Forestry, 19, 388-399.
• 21. WORDRAGEN, M.F., OUWERKERK P.B.F., DONS H.J.M., Agrobacterium rhizogenes Mediated Induction of Apparently Untransformed Roots and Callus in Ciysanthemum, Plant Cell, Tissue and Organ Culture, 30, 149-157, 1992.