Abstract
Yaprakta mozaik simptomları gösteren, mekanik olarak bulaşabilen bir virus kabak bitkisinden izole edilmiştir. Elektron mikroskopta gözlenen virus partikülleri, potyvirus grubunun özelliği olan uzun ince yapıda olan partiküllerdir. Ölçülen 29 partikülden % 65'i 781-813 nm uzunluğunda bulunmuştur. SDS - Immunodifüzyon testleri sonucu, izole edilen virus WMV - 2 virüsü ile benzer reaksiyon vermiştir. Bulunan özellikler ve seroloji testlerinin sonucu, izole edilen virusun WMV - 2 olduğunu göstermiştir.
Keywords
References
- Christie, R.G., 1967. Rapid staining procedures for differentiating plant virus inclusions in epidermal strips. Virology 31, 268-271.
- Christie, R.G., and J.R.Edwardson, 1977. Light and electron microscopy of plant virus inclusions. Fla. Agric. Exp. Stn. Monogr. Ser. 9. 150 p.
- Fischer, H.U., and B.E.L.Lockhart, 1974. Serious losses in cucurbits caused by watermelon mosaic virus in Morocco. Plant Dis. Rep. 58, 143-146.
- Purcifull, D.E., and D.L.Batchelor, 1977. Immunodiffusion tests with sodium dodecyl sulfate (SDS) treated plant viruses and plant viral inclusions. Fla. Agric. Exp. Stn. Tech. Bull. 788. 39 p.
- Purcifull, D.E., S.R.Christie, and D.L.Batchelor, 1975. Preservation of plant virus antigens by freeze-drying. Phytopathology 65, 1202-1205.
- Purcifull, D.E., and E.Hiebert, 1979. Serological distinction of watermelon mosaic virus isolates. Phytopathology 69, 112-116.
- Van Regenmortel, M.H.V., 1971. Watermelon mosaic virus No.13 in Description of Plant Viruses, CMI, Assn, Appl. Biol., Kew, Surrey, England. 4 p.
- Webb, R.E., and H.A.Scott, 1965. Isolation and identification of watermelon mosaic viruses 1 and 2. Phytopathology 55, 895-900.
Abstract
A mechanically transmissible virus was isolated from a squash showing a leaf mosaic symptoms. Virus particles observed with transmission electron microscopy were filamentous particles characteristic of potyviruses. Of 29 particles measured in leaf dips from squash, 65 % ranged from 782 - 813 nm long. This isolate induced systemic mottle or mosaic symptoms on squash and pumpkin. Cylindrical inclusions were seen in cytoplasm of epidermal cells of squash. The SDS- Immunodiffusion tests indicated that an antiserum prepared to an isolate of Water-melon Mosaic Virus - 2 reacted identically whit the unknow. These properties and serological tests showed the mosaic disease was caused by Watermelon Mosaic Virus - 2.
Keywords
References
- Christie, R.G., 1967. Rapid staining procedures for differentiating plant virus inclusions in epidermal strips. Virology 31, 268-271.
- Christie, R.G., and J.R.Edwardson, 1977. Light and electron microscopy of plant virus inclusions. Fla. Agric. Exp. Stn. Monogr. Ser. 9. 150 p.
- Fischer, H.U., and B.E.L.Lockhart, 1974. Serious losses in cucurbits caused by watermelon mosaic virus in Morocco. Plant Dis. Rep. 58, 143-146.
- Purcifull, D.E., and D.L.Batchelor, 1977. Immunodiffusion tests with sodium dodecyl sulfate (SDS) treated plant viruses and plant viral inclusions. Fla. Agric. Exp. Stn. Tech. Bull. 788. 39 p.
- Purcifull, D.E., S.R.Christie, and D.L.Batchelor, 1975. Preservation of plant virus antigens by freeze-drying. Phytopathology 65, 1202-1205.
- Purcifull, D.E., and E.Hiebert, 1979. Serological distinction of watermelon mosaic virus isolates. Phytopathology 69, 112-116.
- Van Regenmortel, M.H.V., 1971. Watermelon mosaic virus No.13 in Description of Plant Viruses, CMI, Assn, Appl. Biol., Kew, Surrey, England. 4 p.
- Webb, R.E., and H.A.Scott, 1965. Isolation and identification of watermelon mosaic viruses 1 and 2. Phytopathology 55, 895-900.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | June 1, 1989 |
| Published in Issue | Year 1989 Volume: 2 Issue: 1 |