ANADOLU SAFRANI (CROCUS SATIVUS L.) SULU EKSTRAKTI İLE GÜMÜŞ NANOPARTİKÜLLERİN (AGNP) YEŞİL SENTEZİ, ANTİOKSİDAN AKTİVİTELERİ VE İNSAN PROSTAT KANSERİ HÜCRELERİ ÜZERİNDEKİ SİTOTOKSİK ETKİLERİ

Year 2025, Volume: 3 Issue: 1, 76 - 85, 01.06.2025

Tolga Özkan , Sueda Nur Ellialtioğlu , Kemal Baş , Serdar Karakurt

Abstract

Safran (Crocus sativus L.), geleneksel tıpta önemli bir yer tutan ve rosetin, pikrokrosin ve safranal gibi farmakolojik bileşenlere sahip bir bitkidir. Bu bileşikler, antidepresan, antikanser, bağışıklık sistemi ve solunum sistemi gibi farklı tedavi alanlarında terapötik etkilere sahiptir. Bu çalışmanın amacı, safran ekstresi ile toplam fenolik, flavonoid ve antioksidan kapasiteyi incelemektir. Ayrıca, safran bitkisinden yeşil sentez yöntemi ile gümüş nanopartiküller (AgNP'ler) sentezlenmiştir. Safran ekstraktının antioksidan kapasitesi DPPH ve CUPRAC yöntemleri ile belirlenmiştir. Sitotoksik etki ise in vitro koşullarda Alamar blue yöntemiyle ölçülmüştür. Yeşil sentezle elde edilen AgNP'ler, UV-vis spektrofotometri ve dinamik ışık saçılma (DLS) teknikleriyle karakterize edilmiştir. Sentezlenen AgNP'lerin UV-vis spektrofotometresinde 410 nm'de absorbsiyon verdiği ve DLS ile 54 nm boyutunda olduğu tespit edilmiştir. Safran ekstresinin toplam fenolik içeriği 153.16 ± 0.03 mg GAE/g ekstre ve flavonoid içeriği ise 1059.218 ± 0.003 mg QE/g ekstre olarak belirlenmiştir. DPPH için safranın IC50 değeri 10.60 ± 0.2 µg/mL, CUPRAC için ise A0.50 değeri 0.313 ± 0.4 µg/mL olarak hesaplanmıştır. Safran ekstresi ve sentezlenen AgNP'ler, PC-3 prostat kanseri hücrelerinde belirgin bir sitotoksik etki göstermiştir. PC-3 hücrelerinde AgNP'lerin IC50 değeri 25.34 ± 1.2 µg/mL olarak ölçülmüştür. Sonuç olarak, safrandan elde edilen gümüş nanopartiküller ve safran ekstresi, antioksidan aktivite göstermekte olup, safran sağlık ve endüstriyel amaçlar için doğal bir potansiyel antioksidan kaynağı ve kanser tedavisinde kullanılabilir bir bileşen olarak değerlendirilebilir.

Keywords

Anadolu Safranı (Crocus sativus L.), Antioksidan Aktivite, Gümüş Nanopartiküller (AgNP), Sitotoksisite, İnsan Prostat Kanseri (PC-3), Yeşil Sentez

Supporting Institution

TUBITAK

Project Number

TUBITAK 2209A

Thanks

Bu proje TUBİTAK 2209A kapsamında desteklenmiştir.

GREEN SYNTHESIS OF SILVER NANOPARTICLES (AGNPS) USING ANATOLIAN SAFFRON (CROCUS SATIVUS L.) AQUEOUS EXTRACT AND THEIR ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECTS ON HUMAN PROSTATE CANCER CELLS

Abstract

Saffron (Crocus sativus L.) is a valuable plant in traditional medicine, known for its pharmacological properties due to its components, such as rosetin, picrocrocin, and safranal. These components have therapeutic effects in various fields, including antidepressant, anticancer, immune, and respiratory systems. This study aimed to investigate the total phenolic, flavonoid, and antioxidant capacity of saffron extract. Additionally, silver nanoparticles (AgNPs) were synthesized using green synthesis from the saffron plant. The antioxidant capacity of saffron extract was determined using the DPPH and CUPRAC methods. The cytotoxic effect was evaluated under in vitro conditions using the Alamar blue assay. The AgNPs synthesized by green synthesis were characterized by UV-vis spectrophotometry and dynamic light scattering (DLS). It was confirmed that the synthesized AgNPs exhibited absorbance at 410 nm in the UV-vis spectrophotometer and had a size of 54 nm by DLS. The total phenolic content of saffron extract was 153.16 ± 0.03 mg GAE/g extract, and the flavonoid content was 1059.218 ± 0.003 mg QE/g extract. The IC50 value of saffron for DPPH was determined as 10.60 ± 0.2 µg/mL, while the A0.50 value for CUPRAC was 0.313 ± 0.4 µg/mL. The saffron extract and synthesized AgNPs exhibited significant cytotoxic effects on PC-3 prostate cancer cells. The IC50 value of AgNPs on PC-3 cells was measured as 25.34 ± 1.2 µg/mL. In summary, the silver nanoparticles derived from saffron and its extract exhibited antioxidant activity and have potential as a natural antioxidant source for health and industrial purposes, as well as a promising component in cancer therapy.

Keywords

Anatolian Saffron (Crocus sativus L.), Antioxidant Activity, Cytotoxicity, Green Synthesis, Human Prostate Cancer (PC-3), Silver Nanoparticles (AgNPs)

Project Number

TUBITAK 2209A

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