Bazı Karayosunu Türlerinin (Cinclidotus riparius (Host ex Brid.) Arn., Calliergonella cuspidata (Hedw.) Loeske, Thamnobryum alopecurum (Hedw.) Gangulee, Leucobryum juniperoideum (Brid.) Müll.Hal., Cirriphyllum crassinervium (Taylor) Loeske & M.Fleisch.) Antibakteriyel Aktivitesinin Belirlenmesi

Year 2016, Volume: 2 Issue: 1-2, 1 - 8, 15.11.2016

GÜRAY Uyar NURCİHAN Hacıoğlu Doğru MUHAMMET Ören , AKIN Çavuş

https://doi.org/10.26672/anatolianbryology.267204

Cited By: 12

Abstract

Bu çalışmada, Zonguldak
çevresinde bol bulunan 5 farklı karayosunu (Cinclidotus
riparius (Host ex Brid.) Arn., Calliergonella
cuspidata (Hedw.) Loeske, Thamnobryum
alopecurum (Hedw.) Gangulee, Leucobryum
juniperoideum (Brid.) Müll.Hal., Cirriphyllum
crassinervium (Taylor) Loeske & M.Fleisch.) ekstraktlarının, 13 farklı bakteri
kültürüne karşı antibakteriyel etkileri incelenmiştir. Ekstreler etanol,
aseton, metanol ve etil asetat (%96) içinde hazırlanmıştır. Karayosunu
ekstraktlarının antibakteriyel aktivitesinin belirlenmesi için Disk Difüzyon
yöntemi uygulanmıştır. Buna ilaveten, sadece çözgenlerin emdirilmiş olduğu
disklerle birlikte standart antibiyotik diskler de kıyas amaçlı kullanılmıştır.

Çalışma sonunda, Escherichia coli ATCC 11230’ye karşı T. alopecurum’un metanol ve aseton
ekstreleri ile L. juniperoideum’un
metanol ektresinin diğer çalışılan tüm karayosunu türlerinden daha fazla antibakteriyel
aktivite gösterdikleri belirlenmiştir. Ayrıca T. alopecurum’un etanolik
ve asetonik ekstrelerinin
aktiviteleri standart olarak çalışılan antibiyotik disklerinden AK30 (Amikasin)’in
etkisinden fazla CTX30 (Sefotaksim)’in etkisi ile aynıydı. Bununla birlikte L. juniperoideum’un metanol ekstresinin antibakteriyel
etkisinin yalnızca AK30 (Amikasin)’den daha yüksek olduğu görülmüştür.

Keywords

Antibakteriyel aktivite, Karayosunu, Bryophyta

Determining Antibacterial Activity of Some Mosses (Cinclidotus riparius (Host ex Brid.) Arn., Calliergonella cuspidata (Hedw.) Loeske, Thamnobryum alopecurum (Hedw.) Gangulee, Leucobryum juniperoideum (Brid.) Müll. Hal., Cirriphyllum crassinervium (Taylor) Loeske & M.Fleisch.)

Abstract

In this study, the antibacterial
activity of 5 different moss extracts (Cinclidotus
riparius (Host ex Brid.) Arn., Calliergonella
cuspidata (Hedw.) Loeske, Thamnobryum
alopecurum (Hedw.) Gangulee, Leucobryum
juniperoideum (Brid.) Müll.Hal. and Cirriphyllum
crassinervium (Taylor) Loeske & M.Fleisch.) which were common in
Zonguldak province and its environs were tested in vitro against the 13
different microorganisms. The extracts were prepared in ethanol, acetone,
methanol, and ethyl acetate (96%). Disk diffusion method was applied for the
determination of antibacterial activity of moss extracts. In addition, standard
antibiotic disks and blank solvent disks were used respectively for comparison
and control.

It is observed at the end
of the study that T. alopecurum
extracts in methanol and acetone and L.
juniperoideum extract in methanol have the greatest antimicrobial
activities against. Escherichia coli
ATCC 11230 among all the other studies moss species. Additionally, the antibacterial activities of T. alopecurum extracts in methanol and acetone were the same with
those of CTX30 (Cefotaxime), but higher than those of AK30 (Amikacin) which
were among the studied standard antibiotic disks. Moreover, the antibacterial effect
of L. juniperoideum extract in
methanol was found higher only than AK30 (Amikacin).

Keywords

Antibacterial activity, Bryophyta, Mosses

References

• Altuner E.M. Canlı K. Akata I. 2014. Antimicrobial Screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme. Journal of Pure and Applied Microbiology. 8:1, 539-545.
• Anna K. Brown D.F. 2001. Quality assurance of antimicrobial susceptibility testing by disc diffusion. Journal of Antimicrobial Chemotherapy. 4, 71–76.
• Asakawa Y. 1990. Bryophyte Development: Physiology and Biochemistry. (Chopra, R. N. and Bhatia, S. C, CRC Press, Florida, pp: 259-89.
• Asakawa Y. 2008. Liverworts-Potential Source of Medicinal Compounds. Current Pharmaceutical Design. 14:29, 3067–3088.
• Banerjee R.D. Sen S.P. 1979. Antibiotic activity of bryophytes. Bryologist. 82: 141–153.
• Basile A. Giordono S. Lopez-Saez J.A. Cobianchi R.C. 1999. Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry. 52:8, 1479–1482.
• Baytop T. 1999. Geçmişte ve bugün Türkiye’de bitkiler ile tedavi. Nobel Tıp Kitap Evi Ltd. Şti. İstanbul.
• Bodade R.G. Borkar P.S. Saiful Arfeen Md. Khobragade CN. 2008. In vitro screening of bryophytes for antimicrobial activity. Journal of Medicinal Plants. 7:4, 23–28.
• Decker E.L. Gorr G. Reski R. 2003. Moss an innovative tool for protein production. BIOforum Europe, No. 2, pp. 96–97.
• Dulger B. Hacıoglu N. Uyar G. 2009. Evaluation of antimicrobial activity of some mosses from Turkey. Asian Journal of Chemistry. 21:5, 4093–4096.
• Frahm J.P. 2001. Biologie der Moose. Spektrum Verlag. Berlin.
• Gould J.C. Bowie J.H. 1952. The determination of bacterial sensitivity of antibiotics. Edinburgh Medical Journal. 59: 178–80.
• Jones F.A. 1996. Herbs-useful plants. Their role in history and today. European Journal Gastroenterology Hepatology. 8: 1227–1231.
• NCCLS, Performance Standards for Antimicrobial Disc Susceptiility Test, Standard, NCCLS M100-S12. Wayne: Pennsylvania.
• Ören M. Bozkaya S. Özçelik A.D. Hazer Y. Uyar G. 2015. Zonguldak ili briyofit florasına katkılar. Anatolian Bryology. 1:1, 34–41
• Rajakaruna N. Harris C.S. Towers G.H.N. 2002. Antimicrobial activity of plants collected from serpentine outcrops in Sri Lanka. Pharmaceutical Biology. 40:3, 235–244.
• Saritas, Y. Sonwa M.M. Iznaguen H. Konig W.A. Muhle H. Mues R. 2001. Volatile constituents in mosses (Musci). Phytochemistry. 57: 443–457.
• Saxena D.K. HarinderA. 2004. Uses of bryophytes. Resonance Article pp. 56–65.
• Zinsmeister H.D. Mues R. 1987. Kayda değer sekonder maddeler kaynağı karayosunları (Ceviren Ulvi Zeybek) ) GIT Labor-Fachzeitschrift. 31: 499–12.