Cytotoxic and Genotoxic Activity of Turkish Pale Liverwort (Chiloscyphus polyanthos (L.) Corda) Against Human Periferal Blood Lymphocytes

Year 2020, Volume: 6 Issue: 1, 1 - 7, 15.06.2020

Recep Kara , Cansu Aydın Songül Budak Diler

https://doi.org/10.26672/anatolianbryology.631973

Abstract

Pale Liverwort-Chiloscyphus polyanthos is one of the most common aquatic liverworts that growing in or near the watering streams and lakes. It typically formed extensive matting appearance in the water or on the boulders in the water. In this study, Chiloscyphus polyanthos collected from the Erciyes Mountain were extracted using ethyl acetate, methanol, and water-methanol solvents. Cytotoxic and genotoxic effects of extracts against human peripheral blood lymphocytes were investigated. The genotoxic activity was determined by using the Chromosome Aberration Test. The cytotoxic activities of the extracts were determined by the Mitotic index (MI). The results showed that the extracts had cytotoxic effect and not genotoxic effect. According to this result, we estimate that Chiloscyphus polyanthos can be used as medicinal plant.

Keywords

Hepatophyta, Medicinal Plants, Chromosome Aberrations, Sister Chromatid Exchange

Supporting Institution

Niğde Ömer Halisdemir Üniversitesi

Project Number

YULTEP/FEB2014/28

Thanks

This work is supported by the Nigde Omer Halisdemir University (Project No: YULTEP, FEB2014/28).

Türk Soluk Ciğerotu (Chiloscyphus polyanthos (L.) Corda)’nun İnsan Periferal Kan Lenfositlerine Karşı Sitotoksik ve Genotoksik Aktivitesi

Abstract

Chiloscyphus polyanthos, göllerde ve
akarsularda su içinde veya su kenarında yetişen en yaygın akuatik ciğerotlarından
biridir. Tipik olarak, su içerisinde veya su içindeki kayaların üzerinde geniş
paspas görünümü oluşturur. Bu çalışmada, Erciyes Dağı'ndan toplanan Chiloscyphus polyanthos, etil asetat,
metanol ve su-metanol çözücüleri kullanılarak ekstre edildi. Ekstraktların
insan periferal kan lenfositlerine karşı sitotoksik ve genotoksik etkileri
araştırıldı. Genotoksik aktivite, Kromozom Aberrasyon Testi kullanılarak
belirlendi. Ekstraktların sitotoksik aktiviteleri Mitotik indeks (MI) ile
belirlendi. Sonuçlar ekstraktların sitotoksik etkiye sahip olduğunu, genotoksik
etkiye sahip olmadığını gösterdi. Bu sonuca göre Chiloscyphus polyanthos’un tıbbi bitki olarak kullanılabileceğini
tahmin etmekteyiz.

Keywords

Hepatophyta, Tıbbi Bitkiler, Kromozom Aberasyonu, Kardeş Kromatid Değişimi

Project Number

YULTEP/FEB2014/28

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