Year 2020, Volume 6 , Issue 1, Pages 1 - 7 2020-06-15

Cytotoxic and Genotoxic Activity of Turkish Pale Liverwort (Chiloscyphus polyanthos (L.) Corda) Against Human Periferal Blood Lymphocytes
Türk Soluk Ciğerotu (Chiloscyphus polyanthos (L.) Corda)’nun İnsan Periferal Kan Lenfositlerine Karşı Sitotoksik ve Genotoksik Aktivitesi

Recep KARA [1] , Cansu AYDIN [2] , Songül BUDAK DİLER [3]


Pale Liverwort-Chiloscyphus polyanthos is one of the most common aquatic liverworts that growing in or near the watering streams and lakes. It typically formed extensive matting appearance in the water or on the boulders in the water. In this study, Chiloscyphus polyanthos collected from the Erciyes Mountain were extracted using ethyl acetate, methanol, and water-methanol solvents. Cytotoxic and genotoxic effects of extracts against human peripheral blood lymphocytes were investigated. The genotoxic activity was determined by using the Chromosome Aberration Test. The cytotoxic activities of the extracts were determined by the Mitotic index (MI). The results showed that the extracts had cytotoxic effect and not genotoxic effect. According to this result, we estimate that Chiloscyphus polyanthos can be used as medicinal plant.

Chiloscyphus polyanthos, göllerde ve akarsularda su içinde veya su kenarında yetişen en yaygın akuatik ciğerotlarından biridir. Tipik olarak, su içerisinde veya su içindeki kayaların üzerinde geniş paspas görünümü oluşturur. Bu çalışmada, Erciyes Dağı'ndan toplanan Chiloscyphus polyanthos, etil asetat, metanol ve su-metanol çözücüleri kullanılarak ekstre edildi. Ekstraktların insan periferal kan lenfositlerine karşı sitotoksik ve genotoksik etkileri araştırıldı. Genotoksik aktivite, Kromozom Aberrasyon Testi kullanılarak belirlendi. Ekstraktların sitotoksik aktiviteleri Mitotik indeks (MI) ile belirlendi. Sonuçlar ekstraktların sitotoksik etkiye sahip olduğunu, genotoksik etkiye sahip olmadığını gösterdi. Bu sonuca göre Chiloscyphus polyanthos’un tıbbi bitki olarak kullanılabileceğini tahmin etmekteyiz.


  • Alam A. 2012. Some Indian Bryophytes known for their biologically active compounds. International Journal of Applied Biology and Pharmaceutical Technology. 3, 239-246.
  • Asakawa Y. Ludwiczuk A. Hashimoto T. 2013a. Cytotoxic and antiviral compounds from bryophytes and inedible fungi. Journal of Pre-clinical and Clinical Research. 7:2, 73-85.
  • Asakawa Y. Ludwiczuk A. Nagashima F. 2013b. Phytochemical and biological studies of bryophytes. Phytochemistry. 91, 52-80.
  • Atherton I. Bosanquet S. D. Lawley M. 2010. Mosses and Liverworts of Britain And Ireland - A Field Guide. British Bryological Society. Britain.
  • Burgess E. Larsen J.L.N. Perry B. 2000. A Cytotoxic sesquiterpene caffeate from the liverwort Bazzania novae-zelandiae. Journal of Natural Products. 63, 537–539.
  • Chandra S. D. Chandra A. Barh A. Pankaj R. Pandey K. Sharma I. P. 2017. Bryophytes: Hoard of remedies, an ethno-medicinal review. Journal of Traditional and Complementary Medicine. 7:1, 94–98.
  • Çolak E. Kara R. Ezer T. Çelik G.Y. Elibol B. 2011. Investigation of antimicrobial activity of some Turkish pleurocarpic mosses. African Journal of Biotechnology. 10, 12905–12908.
  • Dey A. De J.N. 2012. Antioxidative potential of bryophytes: stress tolerance and commercial perspectives: a review. Pharmacologia. 3, 151–159.
  • Dey A. Mukherjee A. 2015. Therapeutic potential of bryophytes and derived compounds against cancer. Journal of Acute Disease. 4:3, 236–248.
  • Komala I.T. Ito F. Nagashima Yagi Y. Asakawa Y. 2010. Cytotoxic, radical scavenging and antimicrobial activities of sesquiterpenoids from the Tahitian liverwort Mastigophora diclados (Brid.) Nees (Mastigophoraceae). Jornal of Natural Medicines. 64, 417–422.
  • Perry N. B. Foster L.M. Lorimer S.D. May B.C. Weavers R.T. 1996. Isoprenyl phenyl ethers from liverworts of the genus Trichocolea: cytotoxic activity, structural corrections and synthesis. Jornal of Natural Medicines. 59, 729-733.
  • Rencüzogulları E. İla H.B. Kayraldiz A. Diler S. B. Yavuz A. Arslan M. Kaya F.F. Topaktas M. 2006. The Mutagenic and Antimutagenic Effects of Ecballium elaterium Fruit Juice in Human Peripheral Lymphocytes. Russian Journal of Genetics. 42:6, 623–627.
  • Saxena D. Harinder K. 2004. Uses of Bryophytes. Resonance. 9:6, 56-65.
  • Suhalka D. 2016. Eco-Friendly Management of Maize Pathogen Thruogh Bryophyte Extract. Journal of Global Pharma Technology. 8:1, 1-4.
  • Toyota M. Saito T. Asakawa Y. 1999. The Absolute Configuration of Eudesmane-type Sesquiterpenoids Found in the Japanese Liverwort Chiloscyphus polyanthus. Phytochemistry. 51, 913-920.
  • Xie C.F. Sun B. Guo D.X. Gao J. Qu J.B. Lou H. X. 2011. Terpenoids from the Chinese Liverwort Chiloscyphus polyanthus. Helvetica Chimica Acta. 94: 3, 534-538.
  • Yağlıoğlu Ş.E. Abay G. Demirtas İ. Yağlıoğlu Ş.A. 2017. Phytochemical screening, antiproliferative and cytotoxic activities of the mosses Rhytidiadelphus triquetrus (Hedw.) Warnst. and Tortella tortuosa (Hedw.) Limpr. Anatolian Bryology 3:1, 31-42.
Primary Language en
Subjects Science
Journal Section Research Articles
Authors

Orcid: 0000-0001-6594-7172
Author: Recep KARA (Primary Author)
Institution: NEVŞEHİR HACI BEKTAŞ VELİ ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
Country: Turkey


Orcid: 0000-0002-5496-8266
Author: Cansu AYDIN
Institution: NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-7156-583X
Author: Songül BUDAK DİLER
Institution: NIGDE OMER HALISDEMIR UNIVERSITY, FACULTY OF SCIENCE AND LETTERS, DEPARTMENT OF BIOTECHNOLOGY
Country: Turkey


Supporting Institution Niğde Ömer Halisdemir Üniversitesi
Project Number YULTEP/FEB2014/28
Thanks This work is supported by the Nigde Omer Halisdemir University (Project No: YULTEP, FEB2014/28).
Dates

Publication Date : June 15, 2020

APA Kara, R , Aydın, C , Budak Di̇ler, S . (2020). Cytotoxic and Genotoxic Activity of Turkish Pale Liverwort (Chiloscyphus polyanthos (L.) Corda) Against Human Periferal Blood Lymphocytes . Anatolian Bryology , 6 (1) , 1-7 . DOI: 10.26672/anatolianbryology.631973