Determination of Antioxidant Activity and Biochemical Content of Homalothecium philippeanum (Spruce) Schimp.

Year 2024, Volume: 10 Issue: 2, 169 - 178, 05.12.2024

Selime Deniz Bozkurt , Dilay Turu , Gizem Gül , Cenker Yaman , Atakan Benek , Kerem Canlı

https://doi.org/10.26672/anatolianbryology.1576833

Abstract

Living organisms produce reactive oxygen species (ROS) during the oxygen processing. ROS damage biomolecules and causes oxidative stress. Antioxidants prevent cellular damage against these harmful effects of ROS by neutralizing free radicals. Medicinal plants provide a rich source of antioxidants to reduce oxidative stress and play an important role in the treatment of diseases. Bioactive compounds, especially polyphenols and flavonoids, protect cells against oxidative damage by neutralizing free radicals. Bryophytes, especially mosses, are one of the plant groups that attract attention in this area. Mosses show biologically active properties with secondary metabolites such as terpenes and flavonoids they contain. In this study, extraction of Homalothecium philippeanum moss with ethanol, methanol, and n-hexane solvents was carried out and the biochemical content analysis of the extracts was investigated. 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester was detected as the major component in the ethanol and methanol extracts of the H. philippeanum. Biologically active alkanes such as Tetradecane and Hexadecane were found in the n-hexane extract. The antioxidant activity of moss ethanol extract was investigated using the DPPH method and the EC50 value was determined as 7.084 mg/ml.

Keywords

Brophytes, Homalothecium philippeanum, Antioxidant Activity, Biochemical Content, GC-MS

Homalothecium philippeanum (Spruce) Schimp.’in Antioksidan Aktivitesinin ve Biyokimyasal İçeriğinin Belirlenmesi

Abstract

Canlı organizmalar, oksijen işleme sürecinde, reaktif oksijen türleri (ROS) üretmektedirler. ROS’lar, biyomoleküllere zarar vererek oksidatif strese neden olmaktadır. Antioksidanlar, serbest radikalleri nötralize ederek ROS’un bu zararlarına karşın hücresel hasarı önlemektedir. Tıbbi bitkiler, oksidatif stresi azaltmak için zengin bir antioksidan kaynağı sunmakta ve hastalıkların tedavisinde önemli bir rol oynamaktadır. Özellikle polifenoller ve flavonoidler gibi biyoaktif bileşikler, serbest radikalleri etkisiz hale getirerek oksidatif hasara karşı hücreleri korur. Briyofitler, özellikle karayosunları, bu alanda dikkat çeken bitki gruplarından olmuşlardır. İçerdikleri terpenler, flavonoidler gibi sekonder metabolitlerle biyolojik olarak aktif özellikler göstermektedirler. Bu çalışma kapsamında, Homalothecium philippeanum karayosununun etanol, metanol ve n-hekzan çözücüleriyle ekstraksiyonu gerçekleştirilmiş ve ekstraktların biyokimyasal içerik analizi araştırılmıştır. Bitkinin, etanol ve metanol ekstraktlarında majör bileşen olarak 1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester tespit edilmiştir. N-hekzan ekstraktında Tetradekan ve Hekzadekan gibi biyolojik olarak aktif alkanlar bulunmuştur. Karayosunu etanol ekstraktının antioksidan aktivitesi DPPH yöntemi kullanılarak araştırılmış ve EC50 değeri 7.084 mg/ml olarak belirlenmiştir.

Keywords

Briyofitler, Homalothecium philippeanum, Antioksidan Aktivite, Biyokimyasal İçerik, GC-MS

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