Eritrosit Transfüzyonunun Anemik Hastalarda Tiyol/Disülfit Homeostazı Üzerine Etkisi

Year 2024, Volume: 7 Issue: 2, 63 - 66, 29.06.2024

Selçuk Coşkun , Ferhat İçme , Yücel Yüzbaşıoğlu , Fatih Tanrıverdi , Gül Pamukçu Günaydın , Cagri Serdar Elgormus , Özcan Erel , Salim Neşelioğlu

https://doi.org/10.54996/anatolianjem.1468418

Abstract

Amaç: Anemi, dokulara oksijen taşınmasını kısıtlar, bu da potansiyel olarak artmış oksidatif stres ile sonuçlanabilir. Bu çalışmada, anemik hastalarda eritrosit transfüzyonunun oksidatif stresin bir göstergesi olan tiyol/disülfit homeostazı üzerindeki etkisinin incelenmesi amaçlandı.

Gereç ve Yöntemler: Çalışmaya acil serviste transfüzyon alan altmış anemik hasta dahil edildi. Eritrosit transfüzyonu öncesi ve sonrası toplanan kan örneklerinde toplam tiyol, nativ tiyol ve disülfid seviyeleri ölçüldü. Aneminin etkilerini izole etmek için, akut/subakut kanama veya aplastik anemi olan hastalar hariç tutularak alt grup analizi de yapıldı.

Bulgular: Total tiyol (374.24±94.84'ten 344.6±88.5'e) ve nativ tiyol (338.91±90.51'den 304.91±90.95'e) konsantrasyonları, eritrosit süspansiyonu transfüzyonu sonrası başlangıca göre anlamlı olarak azaldı (P<0.0001). Ancak, disülfid seviyesi arttı (17.75±6.63'ten 20.97±7.25'e; p = 0.009). Bulgularımız hastalarda oksidatif stresin transfüzyon sonrası arttığını göstermektedir.

Sonuç: Anemik hastalara eritrosit transfüzyonu yapıldığında oksidatif stresin azalacağı düşünülebilir çünkü transfüzyonla oksijen taşıma kapasitesi artar, ancak sonuçlarımız bunun aksini göstermektedir. Sonuçlarımız, transfüzyon alan hastalarda işlemden hemen sonra oksidatif stresin arttığını göstermektedir. Bu durum muhtemelen depolanan eritrosit süspansiyonlarında artan oksidatif stresle ilgili olabilir.

Keywords

Anemi, disulfide, eritrosit transfüzyonu, sulfhidrid bileşikleri, oksidatif stres

Impact of Erythrocyte Transfusion on Thiol/Disulfide Homeostasis in Anemic Patients

Abstract

Aim: Anemia limits oxygen delivery to tissues, potentially leading to increased oxidative stress. This study examined the impact of erythrocyte transfusion on thiol/disulfide homeostasis, a marker of oxidative stress, in anemic patients.

Material and Methods: Sixty anemic patients receiving transfusions in emergency department were included in this study. We measured total thiol, native thiol, and disulfide levels in blood samples collected before and after red blood cell transfusion. To isolate the effects of anemia, a subgroup analysis that excluded patients with acute/subacute hemorrhage or aplastic anemia was also done.

Results: The concentrations of total thiol (374.24±94.84 to 344.6±88.5) and native thiol (338.91±90.51 to 304.91±90.95) significantly decreased after erythrocyte suspension treatment compared to baseline (P<0.0001). However, the disulfide level increased (17.75±6.63 to 20.97±7.25; p = 0.009). Our findings suggest a potential increase in overall oxidative stress.

Conclusion: By giving transfusions to anemic patients one may think that oxidative stress will be reduced due to increased oxygen carrying capacity but our results show the contrary. Our results support that oxidative stress increases in vivo in patients who receive transfusion right after the procedure and this is probably due to increased oxidative stressin stored erythrocyte suspensions.

Keywords

Anemia, disulfides, erythrocyte transfusion, sulfhydryl compounds, oxidative stress

Ethical Statement

We obey all of ethical procedures

Supporting Institution

No supporting İnstution

Thanks

We all thanks for all your help

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