A COMPARATIVE STUDY ON THE EMBRYO-FETAL DEVELOPMENT IN RABBITS AFTER ARTIFICIAL INSEMINATION

Abstract

Objectives: Artificial insemination (AI) is the introduction of spermatozoa into a female's uterus or cervix for the purpose of achieving pregnancy. It is a fertility treatment for humans, in animal breeding and embryo-fetal developmental studies. This study investigated the embryo-fetal development in the rabbit via artificial insemination by evaluating the litter size, reproductive data and external morphological abnormalities after insemination.
Methods: Ten adult female and three male rabbits weighing 1.8 kg and 2 kg, respectively were used. Group A served as
the natural mating, while Group B served as the AI group, and the males served as semen donors. On day 28 of gestation, does were subjected to caesarean section. The ovaries and uteri were removed and examined for the number of corpora lutea and the status of all implantation.
Results: Results showed a significant increase in fetal weight, total fetal number, total resorption sites, number of implantation and total number of live foetuses (p<0.05), while there was no significant increase in parameters like average fetal weight, placenta weight, uterine gravid weight, number of corpora lutea and sex ratio in Group B compared to Group A (p>0.05). A conception rate of 100% was observed in Group A compared to Group B with 80% conception rate. External examination carried out on the foetuses revealed no external abnormalities in both groups.
Conclusion: The results of this study show the clear advantages in the use of rabbits for AI in terms of embryo-fetal developmental studies.

Keywords

• artificial insemination
• caesarean section
• embryo-fetal development

References

1. Theau Clément M. Advances in biostimulation methods applied to
2. rabbit reproduction. World Rabbit Science 2000;8:61–79.
3. Ombelet W, Van Robays J. Artificial insemination history: hurdles
4. and milestones. Facts Views Vis Obgyn 2015;7:137–43.
5. Carluccio A, Robbe D, De Amicis I, Contri A, Tosi U, Russo F,
6. Paoletti M. Artificial insemination in rabbits: laboratory and field
7. trial with three different semen extenders. World Rabbit Science
8. ;12:65–79.

9. Orji BI, Igboeli G, Nwakalor LN. Semen characteristics of Muturu
10. bulls – Bos brachyceros. Theriogenology 1984;22:197–203.
11. Barje PP, Osinowo OA, Dim NI. Effects of diluents, storage and
12. depth of insemination on the fertility of chilled ram spermatozoa.
13. Nigerian Journal of Animal Production 1994;21:42–6.
14. Dede TI, Steinbach J. Artificial insemination of pigs in Nigeria: a
15. preliminary report. Nigerian Journal of Animal Production 1977;4:101–8.
16. Cherms FL. Variations in semen quality and relationship of semen
17. quality to fertility in turkeys. Poult Sci 1968;47:746–54.
18. Rose ID. Semen characteristics, fertility and hatchability in some
19. breeds of cocks. MSc Thesis, Abubakar Tafawa Balewa University, Bauchi, Nigeria, 2000. p. 24–7.
20. Quintela LA, Peña AI, Vega MD, Gullón J, Prieto MC, Barrio M,
21. Becerra JJ, Maseda F, Herrdón PG. Ovulation induction in rabbit
22. does submitted to artificial insemination by adding buserelin to the
23. seminal dose. Reprod Nutr Dev 2004;44:79–88.
24. Morrell JM. Artificial insemination in rabbits. Br Vet J 1995;151:477–88.
25. Walton A. The relation between “density” of sperm-suspension and
26. fertility: as determined by artificial insemination of rabbits. Proc Roy
27. Soc Lond B 1927;101–303.
28. Boussit D. Reproduction et insémination artificielle en cuniculture.
29. Lempdes (France): Association Fran.aise de Cuniculture, INRA;
30. p. 234.
31. Mroueh A, Mastroianni L Jr. Insemination via the intraperitoneal
32. route in rabbits. Fert Steril 1966;17:76–82.
33. Kim JC, Chung MK, Han SS. An artificial insemination technique
34. for obtaining pregnant does in rabbit teratogenicity studies. Korean
35. Journal of Laboratory Animal Science 1996;12:141–6.
36. Wise LD, Beck SL, Beltrame D, Beyer BK, Chahoud I, Clark RL,
37. Clark R, Druga AM, Feuston MH, Guittin P, Henwood SM, Kimmel CA, Lindstrom P, Palmer AK, Petrere JA, Solomon HM, Yasuda M, York RG. Terminology of developmental abnormalities in common laboratory mammals (version 1). Teratology 1997;55:249–92.
38. Drury RAB, Wallington EA. Preparation and fixation of tissues. In:
39. Drury RAB, Wallington EA, editors. Carleton’s histological technique.
40. th ed. Oxford: Oxford University Press; 1980. pp. 41–54.
41. Rommers JM, Meijerhoft R, Noordhuizen JP, Kemp B.
42. Relationships between body weight at first mating and subsequent
43. body development, feed intake, and reproductive performance of
44. rabbit does. J Anim Sci 2002;80:2036–42.
45. Tawfeek MI, El-Gaafary MN. Evaluation of A.I. technique as compared to natural mating in association with some productive and
46. reproductive traits in rabbits. Egyptian Journal of Rabbit Science 1991;1:13–20.
47. Oke UK, Iheanacho VC. Effect of breed and breeding system on
48. reproductive performance of rabbits in a humid tropical environment.
49. Tropical and Subtropical Agroecosystems 2011;14:369–73.
50. Khalifa RM. Mating frequency and artificial insemination in rabbits
51. using vasectomized buck. World Rabbit Science 1994;2:1–5.
52. Kermabon AY, Belair L, Theau-Clément M, Salesse R, Djiane J.
53. Effect of anoestrus and bromocryptine treatment on the expression
54. of prolactin and LH receptors in the rabbit ovary during lactation. J
55. Reprod Fertil 1994;102:131–8.
56. Rebollar PG, Ubilla E, Alvariño JMR, Illera JC, Silvan G. Effect of
57. the level of sexual receptivity on plasma estradiol and the ovulatory
58. response during the postpartum period in the rabbit. [Article in Spanish] Rev Esp Fisiol 1992;48:13–7.
59. Hong JS, Yu WJ, Kim YS, Cho JS, Park MK, Cho SM, Yi H, Cho
60. HJ, Shin HC. Development of a sperm preparation method for artificial
61. insemination in rabbits. Journal of Animal and Veterinary Advances 2012;11:712–8.
62. Ujházy E, Sadlonová I, Dubovick˘ M, Mach M, Múcková M, Flaskárová E. Teratological study of the herbicide 4-chloro-2- methylphenoxyacetic acid in rabbits. J Appl Toxicol 2006;26:368–73.
63. Bonanno A, Costanzo D. The effect of physiological and climatic
64. factors on the main reproductive traits of artificially inseminated rabbits.
65. Rivista di Coniglicoltura 1987;24:33–9.
66. Mach K, Trojan V, Nedvedova V. Fertility, growth and feed consumption in California and New Zealand White purebred and crossbred rabbits. Zivocisna Vyriba 1986;44:109–23.
67. Bencheikh N. Production de sperme et fertilité du lapin male,
68. Oryctolagus cuniculus. Effets de la fréquence de collecte et du type
69. génétique. [Internet] Thèse de doctorat, Ecole Nationale Supérieure
70. Agronomique de Toulouse, France. Available from: http://prodinra.
71. inra.fr/record/110398
72. Bonnano A, Budetta G, Alabiso M, Alicata M. Effect of PGSG and
73. GnRH treatment on the ovulatory efficiency of rabbits. Acta Medica
74. Veterinaria 1990;36:441–51.
75. James WH. Evidence that mammalian sex ratios at birth are partially
76. controlled by parental hormone levels at the time of conception. J
77. Endocrinol 2008;198:3–15.
78. Vega MD, Peña AI, Gullón J, Prieto C, Barrio M, Becerra JJ,
79. Herradón PG, Quintela LA. Sex ratio in rabbits following modified
80. artificial insemination. Anim Reprod Sci 2008;103:385–91.
81. Hafez ES. Growth and survival of blastocysts in the domestic rabbit.
82. II. Quantitative effects of exogenous progesterone following ovariectomy.J Reprod Fertil 1964;7:241–9.
83. Hafez ES. Maternal effects on implantation and related phenomena
84. in the rabbit. Experientia 1965;21:234–7.
85. Wolterbeek APM, Waalkens-Berendsen DH. Oral embryotoxicity/
86. teratogenicity study with trehalose in New Zealand White rabbits.
87. TNO Nutrition and Food Research Institute. TNO Peport 1999;98:1–25.
88. Jubb KVF, Kennedy PC, Palmer NC. Female genital tract. In: Maxie
89. MG, Jubb KVF, Kennedy PC, Palmer N, editors. Pathology of domestic animals. 5th ed. London: Elsevier Saunders; 2007. p. 476.
90. Sendag S, Dinc DA, Celik HA, Aydin I, Wehrend A. Sonographische
91. Verlaufsuntersuchungen an trächtigen Hündinnen. – ein Beitrag zur
92. Bedeutung der Fruchtresorption beim Hund. Tieraerztliche Praxis
93. Kleintiere 2010;3:133–8.
94. Özalp GR, Temizel EM, Özocak-Batmaz E. Clinical, ultrasonography
95. and haematology of aglepristone-inducedmid-gestation pregnancy
96. terminations in rabbits. J S Afr Vet Assoc 2013;84:E1–5.