Effect of zinc sulphate and Essentiale® Forte in managing carbon tetrachloride-induced hepatotoxicity in adult Wistar rats

Year 2017, Volume: 11 Issue: 2, 93 - 98, 20.08.2017

Babatunde E. Arayombo Olarinde S. Adewole David A. Ofusori Taiwo S. Adelodun Stephen S. Olusola Ronald Bejide

Abstract

Objectives: The aim of this study was to assess and compare the ameliorative effect of zinc sulphate and Essentiale® Forte on the histomorphology and lipid histochemistry of carbon tetrachloride (CCl4)-induced hepatic injury in adult Wistar rats.

Methods: Twenty-five adult Wistar rats, weighing between 150 g and 170 g, were used for the study. The animals were

divided into five groups A, B, C, D and E (n=5). Group A received 0.7 ml/kg of olive oil orally. Groups B, C, D and E were administered CCl4 (0.7 ml/kg, orally) for 1 week in 1:1 dilution with olive oil. After CCl4 administration, Group C was treated with Essentiale® Forte (4.5 mg/kg/bw, orally) for four weeks. Group D was treated with zinc sulphate (7 mg/kg/bw, orally) daily for four weeks. Group E received zinc sulphate (7 mg/kg/bw, orally) and Essentiale® Forte (4.5 mg/kg/bw, orally) for a period of 4 weeks, while Group B was left untreated. Animals were left for another one week and subsequently sacrificed under ether anaesthesia. The liver was harvested, weighed and divided into two parts for histological and histochemical studies.

Results: Histological analysis showed that treatment of liver with CCl4 caused hepatotoxicity as marked by presence of inflammatory cells and distortion in the connective tissue fibers, as compared to the ones treated with zinc sulphate and or Essentiale® Forte which restored the hepatic histoarchitecture and protected the liver tissue from fatty and degenerative changes.

Conclusion: This study showed that combination of Essentiale® Forte and zinc supplement offered better ameliorative effects on the liver of Wistar rats following CCl4-induced hepatotoxicity compared with separate administration of either Essentiale® Forte or zinc sulphate.

Keywords

carbon tetrachloride, Essentiale® Forte, hepatotoxicity, liver, zinc sulphate

References

• 1. Cotran RS, Vinay K, Tucker C, Robbins SL. Robbins pathologic basis of disease. 6th ed. Philadelphia: Saunders; 1999.
• 2. Adaramoye OA, Akinloye O. Possible protective effect of kolaviron on CCl4-induced erythrocyte damage in rats. Biosci Rep 2000;259–64.
• 3. World Health Organization. International Programme on Chemical Safety (IPCS). Health and safety guide no. 107. Geneva: WHO; 1998.
• 4. Walker BL, Cooper CD. Air pollution emission factors for medical waste incenerators. J Air Waste Manag Assoc 1992;42:784–91.
• 5. Bojuwoye BU. The burden of viral hepatitis in Africa. West Afr J Med 1997;16:198–203.
• 6. Berger A. What does zinc do? BMJ 2002;325:1062.
• 7. Yadrick MK, Kenney MA, Winterfeldt EA. Iron, copper and zinc status: response to supplementation with zinc or zinc and iron in adult females. Am J Clin Nutr 1989;49:145–50.
• 8. Zhou Z, Sun X, Lambert JC, Saari JT, Kang YJ. Metallothioneinindependent zinc protection from alcoholic liver injury. Am J Pathol 2002;160:2267–74.
• 9. Pilette C, Rousselet MC, Bedossa P, Chappard D, Oberti F, Rifflet H, Maïga MY, Gallois Y, Calès P. Histopathological evaluation of liver fibrosis: quantitative image analysis vs semi-quantitative scores. Comparison with serum markers. J Hepatol 1998;28:439–46.
• 10. Raza M, Al-Shabanah OA, El-Hadiyah TM, Al-Majed AA. Effect of prolonged vigabatrin treatment on haematological and biochemical parameters in plasma, liver, and kidney of Swiss albino mice. Sci Pharm 2002;70:127–37.
• 11. Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. A 90-day oral gavage toxicity study of D-methylphenidate and D,Lmethylphenidate in Sprague-Dawley rats. Toxicology 2002;179: 183–96.
• 12. Tofovic SP, Jackson EK. Effect of long-term caffeine consumption on renal function in spontaneously hypertensive heart failure prone rats. J Cardiovasc Pharmacol 1999;33:360–6.
• 13. Klaassen CD, editor. Casarett and Doull's toxicology: the basic science of poisons. 6th ed. NewYork: McGraw-Hill; 2001.
• 14. Smyth HF, Smyth HF Jr, Carpenter CP. The chronic toxicity of carbon tetrachloride; animalexposure and field studies. J Ind Hyg Toxicol 1936;18:277–97.
• 15. Martelli A, Mereto E, Ghia M. Protective effect of phosphatidylcholine on hepatic lipid peroxidation in rats. Med Sci Res 1989;17: 995–6.
• 16. Guiliano L, Loche A, Ottonello M. Evaluation of the protective activity of phosphatidylcholine in comparing lipid peroxidation processes. [Article in Italian] Boll Soc Ital Biol Sper 1987;63:481–7.