Dose-dependent effect of black mustard seeds (Brassica nigra) extract on the prefrontal cortex of adult Wistar rats

Year 2017, Volume: 11 Issue: 3, 107 - 114, 15.12.2017

Tolulope Timothy Arogundade , Bernard Ufuoma Enaibe Oluwaseun Olaniyi Adigun Foyeke Munirat Adigun İsmail Temitayo Gbadamosi

Abstract

Objectives: Mustard seeds, apart from being a culinary essential, have had medicinal applications dating back to the time of Hippocrates. It has in fact been once mentioned as the greatest herb ever. We explored the dose dependent effects of the crude aqueous extract of Brassica nigra (Black mustard seeds) on the prefrontal cortex of adult Wistar rats.

Methods: 20 adult female rats weighing an average of 180±20 g were used. They were split into 4 groups (n=5); Group A

(received extract at 200 mg/kg body weight), Group B (received extract at 100 mg/kg body weight), Group C (received extract at 50 mg/kg body weight), and Group D (received distilled water ad libitum). All of the animals were subjected to the Y-maze spontaneous alternation test for neurobehavioural analyses following 28-day administration of the extracts. Animals were sacrificed 24 hours after taking the last day of administration.

Results: Our results showed that neurobehavioural analyses are significantly hampered in animals receiving 200 mg/kg extract in comparison to the control group. In treatment groups, increased dose of extract elevated the level of MDA, but reduced the level of SOD. LDH levels were also significantly increased in the 200 mg/kg treated group when comparing with the control. General microarchitecture in the prefrontal cortex of 200 mg/kg Brassica nigra treated group showed signs of karyolysis and pyknosis.

Conclusion: Brassica nigra (black mustard) is not innocuous and therefore it should be consumed in moderation.

Keywords

Brassica nigra; neurobehaviour; neurototoxicity; prefrontal cortex

References

• 1. Duke JA, Bogenschutz-Godwin MJ, duCellier G, Duke PK. Handbook of medicinal herbs. New York (NY): CRC Press; 2000. p.95–6. 2. Felter HW. Monographs extracted from: the eclectic materia medica, pharmacology and therapeutics 1922. Bisbee (AZ): Southwest School of Botanical Medicine; 2001. p. 412–5. 3. Khan BA, Abraham A, Leelamma S. Antioxidant effects of curry leaf, Murraya koenigii and mustards seeds, Brassica juncea in rats fed with high diet. Indian J Exp Biol 1997; 35:148. 4. Joadar A, Das S. Effect of fatty acids isolated from edible oils like mustard, linseed or coconut on astrocytes maturation. Cell Mol Neurobiol 2007;27:973. 5. Billman GE. The effects of omega-3 polyunsaturated fatty acids on cardiac rhythm: a critical assessment. Pharmacol Ther 2013;140:53–80. 6. Yang Y, Raine A. Prefrontal structural and functional brain imaging findings in antisocial, violent, and psychopathic individuals: a metaanalysis. Psychiatry Res 2009;74:81–8. 7. Anderson SW, Bechara A, Damasio H, Tranel D, Damasio A.R. Impairment of social and moral behavior related to early damage in human prefrontal cortex. Nat Neurosci 1999;2:1032–7. 8. Inyang JI, Aniekan-Augusta OE, Olajide TM, Essien A. Effects of ethanolic extract of Brassica juncea (mustard seed) on the brain and kidney tissues of albino Wistar rats. Journal of Biology, Agriculture and Healthcare 2014;22:75–82. 9. Fischer AH, Jacobson K, Rose J, Zeller R. Hematoxylin and eosin staining of tissue and cell sections. Cold Spring Harb Protoc 2008;3:5. 10. Kadar A, Wittmann G, Liposits Z, Fekete C. Improved method for combination of immunocytochemistry and Nissl staining. J Neurosci Methods 2009;184:115–8. 11. Leung AY. Encyclopedia of common natural ingredients used in food, drugs, and cosmetics. New York (NY): Wiley; 1980. 12. Fulton S. Appetite and reward. Front Neuroendocrinol 2010; 31:85–103. 13. Kumar M, Sharma S, Vasudeva N. In vivo assessment of antihyperglycemic and antioxidant activity from oil of seeds of Brassica nigra in streptozotocin-induced induced diabetic rats. Adv Pharm Bull 2013;3:359–65. 14. Selwood T, Jaffe EK Dynamic dissociating homo-oligomers and the control of protein function. Arch Biochem Biophys 2012;519:131–43. 15. Kiasalari Z, Khalili M, Roghani M, Sadeghian A. Antiepileptic and antioxidant effect of Brassica nigra on pentylenetetrazol-induced kindling in mice. Iran J Pharm Res 2012;11:1209–17. 16. Badrul AM, Sarowar HM, Ekramul HM. Antioxidant and antiinflammatory activities of the leaf extract of Brassica nigra. Int J Pharm Sci Res 2012; 2:303–10. 17. Rajamurugan R, Selvaganabathy N, Kumaravel S, Ramamurthy CH, Sujatha V, Thirunavukkarasu C. Polyphenol contents and antioxidant activity of Brassica nigra (L.) Koch. leaf extract. Nat Prod Res 2012;26:2208–10. 18. Coward LA. Neuron physiology. Towards a theoretical neuroscience: from cell chemistry to cognition. In: Cutsuridis V, Taylor JG, editors. Springer series in cognitive and neural systems. Vol 8. New York (NY): Springer; 2013. p. 53–95. 19. Osten P, Margrie TW. Mapping brain circuitry with a light microscope. Nat Methods 2013;10:515–23. 20. Stefanis L, Burke RE, Greene LA. Apoptosis in neurodegenerative disorders. Curr Opin Neurol 1997;10: 299–305.