Year 2019, Volume 13 , Issue 1, Pages 27 - 32 2019-04-29

Effects of chronic unpredictable stress on intestinal morphology in Wistar rats

Ufuk Çorumlu [1] , Osman Özcan Aydın [2] , Emel Ulupınar [3]


Objectives: Stressful events might cause immune dysfunction and trigger various disorders. Adverse effects of acute or chronic stress exposure on the gastrointestinal system have been shown previously in several studies. In this experimental study, we used chronic unpredictable stress (CUS) paradigm to better mimic effects of the intermittent exposure to daily life stress and investigated the morphometric alterations occurring in the small intestines of rats.

Methods: Male Wistar rats were randomly divided into stress and control groups (n=8, each). While stress group was subjected to chronic unpredictable stress protocol for 21 days, control group remained undisturbed. Intestinal tissue samples were obtained from two different regions; one was 3-6 cm away from the pylorus and the other one 3-6 cm prior to the ileocaecal valve. Tissue sections were obtained from paraffin blocks at the thickness of 3 micrometers and stained with hematoxylin-eosin (HE) or periodic acid-Shiff (PAS). The lengths of villi were measured from the basal membrane to the top of the villus. The ratio of degranulating and non-degranulating mast cells per unit area were estimated by point counting method.

Results: The mean villi length in the stress group were significantly higher (p<0.01) than those of the control group. Degranulation to non-degranulation ratio of the mast cells were 40% and 54% in the control and stress groups, respectively.

Conclusion: Animals exposed to chronic unpredictable stress protocol displayed a significant elongation in the villi of small intestines and an increase in the number of degranulating mast cells in the intestinal mucosa. Since activation of mast cells causes releasing of various chemical mediators and growth factors, it is plausible that stressed animals developed an adaptation mechanism to enhance the capacity for absorption and digestion per unit length of the guts. 

chronic unpredictable stress; gastrointestinal system; mast cell; villus length
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Primary Language en
Subjects Health Care Sciences and Services
Journal Section Original Articles
Authors

Author: Ufuk Çorumlu (Primary Author)

Author: Osman Özcan Aydın

Author: Emel Ulupınar

Dates

Publication Date : April 29, 2019

Bibtex @research article { anatomy615125, journal = {Anatomy}, issn = {1307-8798}, eissn = {1308-8459}, address = {}, publisher = {Turkish Society of Anatomy and Clinical Anatomy (TSACA)}, year = {2019}, volume = {13}, pages = {27 - 32}, doi = {}, title = {Effects of chronic unpredictable stress on intestinal morphology in Wistar rats}, key = {cite}, author = {Çorumlu, Ufuk and Aydın, Osman Özcan and Ulupınar, Emel} }
APA Çorumlu, U , Aydın, O , Ulupınar, E . (2019). Effects of chronic unpredictable stress on intestinal morphology in Wistar rats. Anatomy , 13 (1) , 27-32 . Retrieved from https://dergipark.org.tr/en/pub/anatomy/issue/48567/615125
MLA Çorumlu, U , Aydın, O , Ulupınar, E . "Effects of chronic unpredictable stress on intestinal morphology in Wistar rats". Anatomy 13 (2019 ): 27-32 <https://dergipark.org.tr/en/pub/anatomy/issue/48567/615125>
Chicago Çorumlu, U , Aydın, O , Ulupınar, E . "Effects of chronic unpredictable stress on intestinal morphology in Wistar rats". Anatomy 13 (2019 ): 27-32
RIS TY - JOUR T1 - Effects of chronic unpredictable stress on intestinal morphology in Wistar rats AU - Ufuk Çorumlu , Osman Özcan Aydın , Emel Ulupınar Y1 - 2019 PY - 2019 N1 - DO - T2 - Anatomy JF - Journal JO - JOR SP - 27 EP - 32 VL - 13 IS - 1 SN - 1307-8798-1308-8459 M3 - UR - Y2 - 2019 ER -
EndNote %0 Anatomy Effects of chronic unpredictable stress on intestinal morphology in Wistar rats %A Ufuk Çorumlu , Osman Özcan Aydın , Emel Ulupınar %T Effects of chronic unpredictable stress on intestinal morphology in Wistar rats %D 2019 %J Anatomy %P 1307-8798-1308-8459 %V 13 %N 1 %R %U
ISNAD Çorumlu, Ufuk , Aydın, Osman Özcan , Ulupınar, Emel . "Effects of chronic unpredictable stress on intestinal morphology in Wistar rats". Anatomy 13 / 1 (April 2019): 27-32 .
AMA Çorumlu U , Aydın O , Ulupınar E . Effects of chronic unpredictable stress on intestinal morphology in Wistar rats. Anatomy. 2019; 13(1): 27-32.
Vancouver Çorumlu U , Aydın O , Ulupınar E . Effects of chronic unpredictable stress on intestinal morphology in Wistar rats. Anatomy. 2019; 13(1): 32-27.