Comparison of macerations with dermestid larvae, potassium hydroxide and sodium hypochlorite in Wistar rat crania

Year 2019, Volume: 13 Issue: 3, 149 - 154, 31.12.2019

Daniela Botero-gonzález , Marcela Agudelo

Abstract

Objectives: The aim of this study was to determine the most effective maceration method to remove soft tissue without altering bone tissue conformation.

Methods: A comparison was made between maceration with insects and chemical maceration performed on heads of Wistar rats. The sample consisted of 18 biomodels, six of which were macerated by dermestid larvae and the remaining 12 divided into two groups for chemical maceration, one with potassium hydroxide and the other with sodium hypochlorite. In chemical maceration, 1%, 5% and 10% concentrations were used with varying exposure times and temperature.

Results: The ideal method for soft tissue maceration, preserving all bone components, was shown to be maceration with insects. Potassium hydroxide was effective in the removal of soft tissue. However, being a highly corrosive chemical agent, it altered the integrity of the bone tissue. Sodium hypochlorite did not meet the maceration objective.

Conclusion: This research is relevant in its contribution to discussions on appropriate maceration techniques for small bone structures.

Keywords

maceration, soft tissue maceration, bone, osteology, bone tissue integrity, tissue dissolution, chemical maceration, dermestid

Supporting Institution

Universidad del Valle

Thanks

The authors of this study thank the Department of Morphology at Universidad del Valle for authorization to use the laboratory, reagents and biomodels. Many thanks to Professor Oscar Murillo in the Zoology section of the Department of Biology in the Universidad del Valle for his time and help with the Dermestid colony.

References

• AJAYI A, EDJOMARIEGWE O, ISELAIYE OT (2016) A Review of Bone Preparation Techniques for Anatomical Studies. Malaya Journal of Biosciences, 3(2):76-80.
• ATOR GA, ANDREWS JC, MAXWELL DS (1993) Preparation of the human skull for skull base anatomic study. Skull Base Surgery, 3(1):1-6.
• BOYDE A (2012) Scanning Electron Microscopy of Bone. In: Aymen I. Idris (ed.), Bone Research Protocols, Methods in Molecular Biology, vol. 1914. Humana Press, New York, NY.
• COUSE T, CONNOR M (2015). A comparison of maceration techniques for use in forensic skeletal preparations. J Forensic Investigation, 3(1):6.
• GAGE GJ, KIPKE DR, SHAIN W (2012). Whole animal perfusion fixation for rodents. J Vis Exp, 65, e3564.
• GELFAND M, ROSS CM, BLAIR DM, CASTLE WM, WEBER MC (1970) Schistosomiasis of the male pelvic organs. Severity of infection as determined by digestion of tissue and histologic methods in 300 cadavers. Am J Trop Med Hyg, 19(5):779–84.
• GELFAND M, ROSS DM, WEBER MC (1971) Distribution and extent of schistosomiasis in female pelvic organs, with special reference to the genital tract, as determined at autopsy. Am J Trop Med, 20(6):846-9.
• GIBB T (2015). Equipping a diagnostic laboratory. In: Gibb T (ed). Contemporary insect diagnostics.
• FUENTE A, MARTINEZ M, MELLONI L, SALAZAR A, SAAVEDRA A (1994) Craniofacial development in rats with early resection of the zygomatic arch. Plast Reconstr Surg, 95(3): 486-95.
• HILDEBRAND M (1968) Dry skeletons. In: Hildebrand M (ed). Anatomical preparations. London, England.
• KING C, BIRCH W (2015) Assessment of Maceration Techniques Used to Remove Soft Tissue from Bone in Cut Mark Analysis. J Forensic Sci, 60(1):124-135.
• MANN RW, BERRYMAN HE (2012) A Method for Defleshing Human Remains Using Household Bleach. J Forensic Sci, 57(2):440-42.
• MILLER DM, TARPLEY J (1996) An Automated Double Staining Procedure for Bone and Cartilage. Biotechnic & Histochemistry, 71(2):79-83.
• STEADMAN DW, DIANTONIO LL, WILSON JJ, SHERIDAN KE, TAMMARIELLO SP (2006) The Effects of Chemical and Heat Maceration Techniques on the Recovery of Nuclear and Mitochondrial DNA from Bone. J Forensic Sci, 51(1):11-7.
• RENAUD R, BRETTES P, CASTANIER C, LOUBIERE R (1972). Placental Bilharziasis. Int J Gynaecol Obstet, 10:24–30.