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Ascochyta Yanıklığı Ascochyta rabiei Pass. Labr. 'na Dayanımları Farklı Nohut Çeşitlerinde Oransal Su, Kuru Madde ve Prolin Miktarlarındaki Değişimler

Year 2001, Volume: 07 Issue: 01, 119 - 124, 01.01.2001
https://doi.org/10.1501/Tarimbil_0000000266

Abstract

Bu çal ışmada, Ascochyta Yan ı kl ığı hastal ığı n ı n çı kma şiddetine bağ l ı olarak meydana gelen hastal ığ a duyarl ı , Can ı tez 87 ve dayan ı kl ı , ILC 195 nohut çeşitlerinin yapraklar ı nda hastal ı k stresinin Oransal Su içeri ğ i % O. S. İ . , kuru maddeye % K..M. etkisi ile ayn ı çeşitlerin yaprak, gövde ve köklerinde de ğ işen prolin miktarlar ı incelenmi ştir. Duyarl ı çeşide ait yapraklarda %O. S. İ . dayan ı kl ı çeşide ait yapraklardan daha yüksektir. Her iki nohut çe şidinin enfekteli yapraklar ı nda da % 0.S.I.' nin daha az oldu ğ u bulunmuştur. Her iki çeşidin enfekteli yapraklar ı nda 8. günde kuru madde % K.. M. 'de art ış olduğu tespit edilmiştir. Prolin miktar ı yapraklarda , gövde ve köklerden daha fazla bulunmuştur. Enfekteli bitkilerde su eksikli ğine bağl ı olarak prolin miktar ı nda art ış her iki nohut çeş idinde de tespit edilmiştir. Hastal ığı n ortaya ç ı kmas ı na bağl ı olarak duyarl ı çeşitte en fazla prolin 8. günde bulunurken dayan ı kl ı çeş itte 11. günde daha fazla prolin birikimi oldu ğu tespit edilmiştir. Hastal ığı n ç ı kma şiddetine bağ l ı olarak prolin miktar ı n ı n değişimi Ascochyta Yan ı kl ığı na karşı dayan ı mda belirleyici bir parametre olabilir.

References

  • Anonymous, 1998. Tar ı msal Yap ı 1996. T. C. Başbakanl ı k Devlet istatistik Enstitüsü, Ankara, s. 591.
  • Aspinall D. and L. G. Paleg, 1981. Proline accumulation: physiological aspects. In L.G. Paleg Aspinall D, eds. The physiology and biochemistry of drought resistance in plants. Academic Press, Sydney, Australia. s. 206- 241.
  • Bates L. S., R. P. Walderen, I. D. Teare, 1973. .Rapid determination of free proline for water stress studies Plant and Soil 39: 205- 208.
  • Dolar, F. S., A. Gürcan, 1993. The role of the phytoalexins on the resistance to Chickpea Blight ( Ascochyta rabiei (Pass.) Labr.) in Chickpeas. J. Turk. Phytopatho, 22: 17-26.
  • Dolar, F. S., A. Gürcan, 1995. Nohut Antraknozu (Ascochyta rabiei (Pass.) Labr.)'na dayan ı kl ı ve duyarl ı nohut hatları nda fizyolojik farkl ı l ı klar. Tr. J. of Agricul. and Foresty 19: 31- 37.
  • Erdiller G., C. J. Smith, F. S. Dolar, B. Akba ş, Y. Z. Kat ı rc ı oğlu, İ . Ö. Elibüyük ve E. Seçer, 1998. Ascochyta rabiei' ye dayan ı kl ı ve duyarl ı nohut çeşitlerinde Beta-1, 3- gluconase ce chitinase aktivitelerinin belirlenmesi. Türkiye VIII. Fitopatoloji Kongresi Bildirileri, 21- 25 Eylül 1998, ANKARA. 95- 100.
  • Eser, D. ve H. Soran, 1978 . Yerli ve yabanc ı kökenli nohut çeşitlerinin Orta Anadolu çevre ko ş ulları nda erkencilik, verimlilik ve hastal ı klara dayan ı kl ı l ı k yönünden mukayeseli incelenmesi. A. O. Z. F. Yay ı n No: 684, Ankara.
  • Goldman , R. N., Z. Kraly and M. Zaitlin, 1967. The biochemistry and physiology of infectious plant disease. D. Van Nostrad Company, Inc. Princeton, New Jersey, Toronto, London, Melborne.
  • Goyal A. 1987. Effects of water stress on glycolate metabolism in leaves of rice seedlings ( Oryza sativa). Physiol. Plantarum 69: 289- 294.
  • Grumet R., S. A. Rulon and A. D. Hanson, 1987. Growth and yield of barley isopopulations differing in solute potential. Crop Science 27: 991- 995.
  • Handa, S., A. T. Handa, P. M. Hasegawa, and R. A. Bressan, 1986. Proline accumulation and the adaptation of cultured plant cells to stress. Plant Physiol. , 80: 938- 945.
  • Hwang , B. K., K. D. Kim and B. K. Young, 1989. Carbonhydrate composition and acid invertase activity in Rice leaves infected with Pyricularia oryzae. J. Phytopathology, 125: 124-.132.
  • Ingman, J. L. 1962. Induced and constituve isoflavonids from stems of Chickpeas ( Cicer arietium L.) inoculated with spores of Helminthosporium carbonumullstrup. Phytopath. Z. 87: 353- 367.
  • Iringoyen J. J., D. W. Emerich and M. Sachez-Diaz, 1992. Water stress induced changes in concentrations of proline and total solubl sugars in nodulated alfalfa ( Medicago sativa)plants. Physiol. Plantarum 84: 55- 60.
  • Jones, H. G., T. J. Flowers and M. B. Jones, 1993. Plants under stress. Biochemistry, Physiology and Ecology and their application to plant improvement. Cambridge University Press, 257s.
  • Joyce P. A., D. Aspinall, and L. G. Paleg, 1992. Photosynthesis and accumulation of proline in response to water response. Aust. J. Plant Physiol. , 19: 249- 261.
  • Kuo , C. G., H. M. Chen and L. H. Ma, 1986. Effect of high temperature on proline content in tomato floral bunds and leaves. J. Amer. Soc. Hort. Sci. 111(5): 746- 750.
  • Liu C. H., I. Frmmhold, and S. Kluge, 1992. Increase of proline contents in tobacco plants after virüs infection. J. of Plant Dis. and Prot. , 99: 564- 568.
  • Lone, M. I., J. S. H. Kueh, R. G. Wyn Jones and S. W. J. Bright, 1987. lnfluence of proline and glycinebetain on salt tolerance of cultured barley embryos. J. of Experimental Botany 38: 479- 490.
  • Misaghi, I. J. 1988, Physiology and biochemistry of plantpathogen interaction. Plenum Press , New- York, London.
  • Morgan, J. M. 1992. Osmotic components and properties associated with genotypic differences in osmoregulation in Wheat. Aust. J. Plant Physiol. , 19: 67- 76.
  • Nikolopoulus L. and Y. Manetas, 1991, Compatible solutes and in vitro stability of Salsola soda enzymes proline in compatibility. Phytochem., 30(2): 411- 413.
  • Ochsenbauer, S. A. and J. Ochsenbauer, 1988. Levels of free proline in ornamental plants: I. lnfluence of plant age, leaf region in Saintpaulia and Chrysanthemum. J. Plant Physiol., 132: 758- 761.
  • Öncel, I. 1991. Düşük s ı cakl ı klarda Lahana (Brassica oleseea L.) serbest prolin birikimi. Ondokuz May ı s Üniversitesi, Fen Dergisi , 3 (1): 25- 43.
  • Öncel, I., A. S. Üstün,.and Y. Kele ş, 1996. Proline accumulation in peppers (Capsicum annuum L.) resistant and susceptible to root rot (Phytophthora capsici Leon.) . Tr J . of Botany 20: 489- 495.
  • Patel J. A., and B. Vora, 1985. Free proline accumulation in drought- stressed plants. Plant and Soil 84: 427- 429.
  • Rudolph, A. S., J. H. Crowe and L. J. Crowe, 1986. Effects of three stabilizing agents-proline, betaine and trehalose- on membrane phospholipids. Arch. Biochem. Biophys. 245, 134- 143.
  • Samaras, Y., R. A. Bressan, L. N. Csonka, M. G. Garcia-Rios, ve ark., 1995. Proline accumulation during drought and salinity, in Environmental plant metabolism (ed. Smirnoff, N.), Bios Scientifı c Pub. , OXFORD. s.161- 187.
  • Sequeira, L. 1983. Mecanism of induced resistance in plants. Ann. Rev. Microbiol. , 37: 51- 79.
  • Singh, K. B., G. C. Hawtin, Y. L. Nene and M. V. Reddy, 1981. Resistance in Chickpeas to Ascochyta rabiei. Plant Disease. 65: 586- 587.
  • Singh, K. B. and M. V. Reddy, 1989. Genetics of resistance to Ascochyta blight in four Chickpea lines. Crop Sci. 29: 657- 659.
  • Smart R. E. and G. E. Bingham, 1974. Rapid estimates of relative water content . Plant Physiol., 53: 258- 260.
  • Stewart C. R. and G. Voetberg, 1985. Relationship between stress induced ABA and proline accumulations and ABAInduced proline accumulation in excised Barley leaves. Plant Physiol. , 79: 24- 27.
  • Taylor, A. A., J. D. E. Felice and D. C. Havill, 1982. N İ trogen metabolism in Poterium sanguisorva during water stress. New Phytologist. 90: 19- 25.
  • Venekamp J. H. and J. T. M. Koot, 1988. The sources of free proline and asparagine in field bean plants , Vicia faba L. , during and after a short period of water withholding. J. Plant Physiol, 132: 102- 109.
  • Venekamp J. H., J. E. M. Lampe and J. T. M. Koot, 1989. Organic acids as sources for Drought- induced proline synthesis in field bean plants, Vicia faba L. J. Plant Physiol. , 133: 654- 659.
  • Venekamp J. H. 1989. Regulation of cytosol acidy in plants under conditions of drought. Physiol. Plantarum 76: 112- 117.
  • Weigand , F., J. Köster, H. C. Weltzien and W. Barz, 1986. Accumulation of phytoalexins and isoflavone glucosides in a resistant and a susceptible cultivar of Cicer arientium during infection with Ascochyta rablei. J. Phytopathology. 115: 214- 221.
  • Zaki, A. I. and R. D. Durbin, 1965. Effect of Bean Rust on the translocation of photosynthesis products from diseased leaves. Phytopathology. 53: 528- 529.

Changes in the Relative Water Content, Dry Matter and Proline Amount in the Ascochyta Blight Ascochyta rabiei Pass. Labr. Resistant and Susceptible Chickpea Cultivars

Year 2001, Volume: 07 Issue: 01, 119 - 124, 01.01.2001
https://doi.org/10.1501/Tarimbil_0000000266

Abstract

In this study, the effect of Ascochyta blight Ascochyta rabiei Pass. Labr. disease stress on the relative water content RWC % and dry matter D. W % content of susceptible Can ı tez 87 and resistant ILC 195 cultivars were investigated. Proline amount alternations in the leaves, stems and roots of the same cultivars were also studied. RWC % was higher in the leaves of susceptible cultivar than of resistant cultivar. RWC % was found to be less in both cultivar leaves which were infected. The amount of dry matter content increased in the infected leaves of both cultivars in the 8 thday. The amount of proline was higher in leaves than stems and roots. In infected resistant and susceptible cultivars the amount of proline increased as the water content decreased. In susceptible cultivar the amount of proline was found to be highest in the 8 thday, however, the amount of proline accumulation was higher than 11 th day. The changes in the amount of proline could be a determinative factor in resistance to the disease.

References

  • Anonymous, 1998. Tar ı msal Yap ı 1996. T. C. Başbakanl ı k Devlet istatistik Enstitüsü, Ankara, s. 591.
  • Aspinall D. and L. G. Paleg, 1981. Proline accumulation: physiological aspects. In L.G. Paleg Aspinall D, eds. The physiology and biochemistry of drought resistance in plants. Academic Press, Sydney, Australia. s. 206- 241.
  • Bates L. S., R. P. Walderen, I. D. Teare, 1973. .Rapid determination of free proline for water stress studies Plant and Soil 39: 205- 208.
  • Dolar, F. S., A. Gürcan, 1993. The role of the phytoalexins on the resistance to Chickpea Blight ( Ascochyta rabiei (Pass.) Labr.) in Chickpeas. J. Turk. Phytopatho, 22: 17-26.
  • Dolar, F. S., A. Gürcan, 1995. Nohut Antraknozu (Ascochyta rabiei (Pass.) Labr.)'na dayan ı kl ı ve duyarl ı nohut hatları nda fizyolojik farkl ı l ı klar. Tr. J. of Agricul. and Foresty 19: 31- 37.
  • Erdiller G., C. J. Smith, F. S. Dolar, B. Akba ş, Y. Z. Kat ı rc ı oğlu, İ . Ö. Elibüyük ve E. Seçer, 1998. Ascochyta rabiei' ye dayan ı kl ı ve duyarl ı nohut çeşitlerinde Beta-1, 3- gluconase ce chitinase aktivitelerinin belirlenmesi. Türkiye VIII. Fitopatoloji Kongresi Bildirileri, 21- 25 Eylül 1998, ANKARA. 95- 100.
  • Eser, D. ve H. Soran, 1978 . Yerli ve yabanc ı kökenli nohut çeşitlerinin Orta Anadolu çevre ko ş ulları nda erkencilik, verimlilik ve hastal ı klara dayan ı kl ı l ı k yönünden mukayeseli incelenmesi. A. O. Z. F. Yay ı n No: 684, Ankara.
  • Goldman , R. N., Z. Kraly and M. Zaitlin, 1967. The biochemistry and physiology of infectious plant disease. D. Van Nostrad Company, Inc. Princeton, New Jersey, Toronto, London, Melborne.
  • Goyal A. 1987. Effects of water stress on glycolate metabolism in leaves of rice seedlings ( Oryza sativa). Physiol. Plantarum 69: 289- 294.
  • Grumet R., S. A. Rulon and A. D. Hanson, 1987. Growth and yield of barley isopopulations differing in solute potential. Crop Science 27: 991- 995.
  • Handa, S., A. T. Handa, P. M. Hasegawa, and R. A. Bressan, 1986. Proline accumulation and the adaptation of cultured plant cells to stress. Plant Physiol. , 80: 938- 945.
  • Hwang , B. K., K. D. Kim and B. K. Young, 1989. Carbonhydrate composition and acid invertase activity in Rice leaves infected with Pyricularia oryzae. J. Phytopathology, 125: 124-.132.
  • Ingman, J. L. 1962. Induced and constituve isoflavonids from stems of Chickpeas ( Cicer arietium L.) inoculated with spores of Helminthosporium carbonumullstrup. Phytopath. Z. 87: 353- 367.
  • Iringoyen J. J., D. W. Emerich and M. Sachez-Diaz, 1992. Water stress induced changes in concentrations of proline and total solubl sugars in nodulated alfalfa ( Medicago sativa)plants. Physiol. Plantarum 84: 55- 60.
  • Jones, H. G., T. J. Flowers and M. B. Jones, 1993. Plants under stress. Biochemistry, Physiology and Ecology and their application to plant improvement. Cambridge University Press, 257s.
  • Joyce P. A., D. Aspinall, and L. G. Paleg, 1992. Photosynthesis and accumulation of proline in response to water response. Aust. J. Plant Physiol. , 19: 249- 261.
  • Kuo , C. G., H. M. Chen and L. H. Ma, 1986. Effect of high temperature on proline content in tomato floral bunds and leaves. J. Amer. Soc. Hort. Sci. 111(5): 746- 750.
  • Liu C. H., I. Frmmhold, and S. Kluge, 1992. Increase of proline contents in tobacco plants after virüs infection. J. of Plant Dis. and Prot. , 99: 564- 568.
  • Lone, M. I., J. S. H. Kueh, R. G. Wyn Jones and S. W. J. Bright, 1987. lnfluence of proline and glycinebetain on salt tolerance of cultured barley embryos. J. of Experimental Botany 38: 479- 490.
  • Misaghi, I. J. 1988, Physiology and biochemistry of plantpathogen interaction. Plenum Press , New- York, London.
  • Morgan, J. M. 1992. Osmotic components and properties associated with genotypic differences in osmoregulation in Wheat. Aust. J. Plant Physiol. , 19: 67- 76.
  • Nikolopoulus L. and Y. Manetas, 1991, Compatible solutes and in vitro stability of Salsola soda enzymes proline in compatibility. Phytochem., 30(2): 411- 413.
  • Ochsenbauer, S. A. and J. Ochsenbauer, 1988. Levels of free proline in ornamental plants: I. lnfluence of plant age, leaf region in Saintpaulia and Chrysanthemum. J. Plant Physiol., 132: 758- 761.
  • Öncel, I. 1991. Düşük s ı cakl ı klarda Lahana (Brassica oleseea L.) serbest prolin birikimi. Ondokuz May ı s Üniversitesi, Fen Dergisi , 3 (1): 25- 43.
  • Öncel, I., A. S. Üstün,.and Y. Kele ş, 1996. Proline accumulation in peppers (Capsicum annuum L.) resistant and susceptible to root rot (Phytophthora capsici Leon.) . Tr J . of Botany 20: 489- 495.
  • Patel J. A., and B. Vora, 1985. Free proline accumulation in drought- stressed plants. Plant and Soil 84: 427- 429.
  • Rudolph, A. S., J. H. Crowe and L. J. Crowe, 1986. Effects of three stabilizing agents-proline, betaine and trehalose- on membrane phospholipids. Arch. Biochem. Biophys. 245, 134- 143.
  • Samaras, Y., R. A. Bressan, L. N. Csonka, M. G. Garcia-Rios, ve ark., 1995. Proline accumulation during drought and salinity, in Environmental plant metabolism (ed. Smirnoff, N.), Bios Scientifı c Pub. , OXFORD. s.161- 187.
  • Sequeira, L. 1983. Mecanism of induced resistance in plants. Ann. Rev. Microbiol. , 37: 51- 79.
  • Singh, K. B., G. C. Hawtin, Y. L. Nene and M. V. Reddy, 1981. Resistance in Chickpeas to Ascochyta rabiei. Plant Disease. 65: 586- 587.
  • Singh, K. B. and M. V. Reddy, 1989. Genetics of resistance to Ascochyta blight in four Chickpea lines. Crop Sci. 29: 657- 659.
  • Smart R. E. and G. E. Bingham, 1974. Rapid estimates of relative water content . Plant Physiol., 53: 258- 260.
  • Stewart C. R. and G. Voetberg, 1985. Relationship between stress induced ABA and proline accumulations and ABAInduced proline accumulation in excised Barley leaves. Plant Physiol. , 79: 24- 27.
  • Taylor, A. A., J. D. E. Felice and D. C. Havill, 1982. N İ trogen metabolism in Poterium sanguisorva during water stress. New Phytologist. 90: 19- 25.
  • Venekamp J. H. and J. T. M. Koot, 1988. The sources of free proline and asparagine in field bean plants , Vicia faba L. , during and after a short period of water withholding. J. Plant Physiol, 132: 102- 109.
  • Venekamp J. H., J. E. M. Lampe and J. T. M. Koot, 1989. Organic acids as sources for Drought- induced proline synthesis in field bean plants, Vicia faba L. J. Plant Physiol. , 133: 654- 659.
  • Venekamp J. H. 1989. Regulation of cytosol acidy in plants under conditions of drought. Physiol. Plantarum 76: 112- 117.
  • Weigand , F., J. Köster, H. C. Weltzien and W. Barz, 1986. Accumulation of phytoalexins and isoflavone glucosides in a resistant and a susceptible cultivar of Cicer arientium during infection with Ascochyta rablei. J. Phytopathology. 115: 214- 221.
  • Zaki, A. I. and R. D. Durbin, 1965. Effect of Bean Rust on the translocation of photosynthesis products from diseased leaves. Phytopathology. 53: 528- 529.
There are 39 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

A.sülün Üstün This is me

Sara Dolar This is me

Publication Date January 1, 2001
Submission Date January 1, 2001
Published in Issue Year 2001 Volume: 07 Issue: 01

Cite

APA Üstün, A., & Dolar, S. (2001). Ascochyta Yanıklığı Ascochyta rabiei Pass. Labr. ’na Dayanımları Farklı Nohut Çeşitlerinde Oransal Su, Kuru Madde ve Prolin Miktarlarındaki Değişimler. Journal of Agricultural Sciences, 07(01), 119-124. https://doi.org/10.1501/Tarimbil_0000000266

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