Rat Modelinde Laminektomi Sonrası Epidural Fibrozisin Topikal Aminoguanidin Kullanılarak İnhibisyonu

Year 2018, Volume: 7 Issue: 1, 18 - 22, 01.04.2018

M Arif Aladağ

Abstract

Amaç: Bu deneysel çalışmada, laminektomi sonrası epidural fibrozisin indüklenebilir nitrik oksit sentaz iNOS inhibitörü olan aminoguanidin topikal uygulanması ile önlenmesini araştırdık. Gereç ve Yöntem: Bu çalışmada her biri 10 rat içeren 3 grup oluşturuldu. kullandık. Tüm ratlarda lomber laminektomi yapıldı. Kontrol grubu grup1 epidural mesafeye herhangi bir malzeme yerleştirilmeden kapatıldı. Spongostan ve spongostan artı aminoguanidin gruplarında epidural mesafeye, kapatmadan önce sırasıyla salin 0.9 % NAC emdirilmiş spongostan ve aminoguanidin 50 mg/kg B. W. emdirilmiş spongostan yerleştirildi. Bu hayvanların hepsi operasyondan sonraki 14. günde sakrifiye edildi. Her bir ratda histolojik olarak ektradural fibrozis ve kompresyon değerlendirildi. Sonuçlar: Spongostan artı aminoguanidin grubunda fibröz proliferasyon veya kompresyon yokken, kontrol ve spongostan gruplarında, sırasıyla belirgin ve orta derecede fibrozis ve kompresyon mevcuttu. Sonuç: Aminoguanidinin, laminektomi sonrası epidural fibrozis ve kompresyonun önlenmesinde ideal bir inhibitör olabileceği gösterildi

Keywords

Rat, laminektomi, epidural fibrozis, aminoguanidin, spongostan

Inhibition of Epidural Fibrosis After Laminectomy Using Topical Aminoguanidine in A Rat Model

Abstract

Aim: In this experimental study, we investigated prevention of epidural fibrosis following laminectomy with topical application of aminoguanidine, a selective inhibitor of inducible nitric oxide synthase iNOS . Materials and Methods: We used 3 groups each containing 10 male wistar rats. All rats were made laminectomy in lumbar spine. The control group was closed without placing any material on the epidural space. In the spongostan and spongostan plus aminoguanidine, groups the saline 0.9 % NaCl soaked spongastan and aminoguanidine 50 mg/kg B.W. soaked spongostan were placed epidurally respectively before closing. All of these animals were sacrificied on the 14th days after operation. The extradural fibrosis and compression in every animal were evaluated by histological examinations. Results: While there was not any fibrous proliferation or compression in the group of spongostan plus aminoguanidine group. There were obvious and moderate extradural fibrosis and compression in the control and spongostan groups. Conclusion: It was suggested that aminoguanidine may be an ideal inhibitor in the prevention of epidural fibrosis and compression following laminectomy

Keywords

Rat, laminectomy, epidural fibrosis, aminoguanidine, spongostan

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