Kistik Ekinokoklu Koyunlarda IFN-γ Seviyelerinin İmmunohistokimyasal Olarak Değerlendirilmesi

Yıl 2023, Cilt: 2 Sayı: 1, 37 - 42, 27.06.2023

Emin Karakurt , Şemistan Kızıltepe , Ayfer Yıldız

Öz

Güncel çalışmada kistik ekinokokkozisli (KE) koyunların akciğerlerinde parazite karşı gelişen hücresel immun yanıtın detaylandırılması amacıyla sağlıklı hayvanlara kıyasla enfekte hayvanlardaki interferon-gamma (IFN-γ) düzeyleri immunohistokimyasal yöntemlerle değerlendirilmiştir. Bu çalışmanın materyalini Kafkas Üniversitesi Veteriner Fakültesi Patoloji Anabilim Dalı’na rutin tanı amacıyla ölü olarak getirilen ortalama 10 adet koyundan alınan akciğer dokusu örnekleri oluşturmuştur. 10 adet sağlıklı ve herhangi bir patolojik lezyon sergilemeyen koyunlardan alınan akciğer dokusu örnekleri de kontrol amacıyla kullanılmıştır. Dokulardaki histopatolojik değişikliklerin değerlendirilebilmesi amacıyla kesitlere Hematoksilen & Eozin boyaması yapıldı. Avidin-Biotin Peroksidaz-Tekniği kullanılarak IFN-γ ticari antikoru ile üretici firmanın prosedürlerine uygun olarak boyandı. Makroskobik muayenede akciğerin hemen hemen tüm loplarına yayılmış, irili ufaklı oval şekilli kistik yapılar tespit edildi. Akciğerlerin histopatolojik incelemelerinde kistlerin fibröz bir kapsülle çevrelendiği ve fibröz kapsülün hemen alt kısmında konsantirik lamellasyon gösteren kist çeperinin olduğu gözlendi. Kontrol grubuna kıyasla IFN-γ düzeylerinin KE grubunda istatistiki anlamda artış gösterdiği belirlendi. IFN-γ düzeylerindeki bu denli artışın KE gibi paraziter hastalıkların teşhisinde bu belirtecin dikkate değer bir sitokin olduğunu ortaya koymaktadır. Paraziter enfeksiyona karşı geliştirilen hücresel immun yanıtta baskın bir rol oynayan dev hücreleri ve makrofajlar gibi fagositlerde IFN-γ boyamaların çok daha şiddetli olması da bu pro-inflamatuar sitokinin anti-ekinokok tedavisinde etkili olabileceğini göstermektedir.

Anahtar Kelimeler

Hücresel Savunma, İnterferon-γ, Kistik Ekinokokkozis, Koyun, Sitokin

Immunohistochemical Evaluation of IFN-γ levels in sheep with cystic echinococcosis

Öz

In the present study, with an aim to demonstrate in detail the cellular immune response given to the parasite in the lungs of sheep infected with cystic echinococcosis (CE), a comparative assessment was made of the interferon-gamma (IFN-γ) levels of healthy and infected animals using immunohistochemical methods. The material of this study consisted of lung tissue samples taken from an average of 10 dead sheep brought to Faculty of Veterinary Medicine Department of Pathology for routine diagnosis. Lung tissue samples taken from 10 healthy sheep without any pathological lesions were also used for control purposes. Hematoxylin & Eosin staining was applied to the sections in order to evaluate the histopathological changes in the tissues. Stained with IFN-γ commercial antibody using the Avidin-Biotin Peroxidase-Technique in accordance with the manufacturer's procedures. Macroscopic examination revealed large and small oval-shaped cystic structures that spread to almost all lobes of the lung. In the histopathological examination of the lungs, it was observed that the cysts were surrounded by a fibrous capsule and there was a concentric lamellated cyst wall just below the fibrous capsule. It was determined that IFN-γ levels increased statistically in the CE group compared to the control group. Such an increase in IFN-γ levels reveals that this marker is a remarkable cytokine in the diagnosis of parasitic diseases such as CE. The fact that IFN-γ staining is much more severe in phagocytes such as giant cells and macrophages, which play a dominant role in the cellular immune response developed against parasitic infection, shows that this pro-inflammatory cytokine may be effective in anti-echinococcal therapy.

Anahtar Kelimeler

Cellular Defense, Cystic Echinococcosis, Cytokine, Interferon-γ, Sheep

Kaynakça

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