A Methodological Approach Related to the Decontamination Process from Ancient Skeletal Samples in Different Conditions

Year 2017, , 31 - 47, 30.12.2017

Apak Merve İyras Yeşim Doğan

https://doi.org/10.1501/antro_0000000346

Abstract

Understanding that DNA can be obtained from ancient remains has made valuable contribution to the work of anthropologists. Despite this important contribution, ancient DNA analysis brings many challenges due to the nature of the studied specimens. The most important problem in these studies is to select well-preserved samples and to obtain the best quality DNA possible from these samples. Ancient samples should be purified from external contaminants that they are exposed to at different stages prior to DNA extraction. The success of the purification process is of great importance in terms of the reliability of the results. Results obtained at the end of the process should be defended by researcher showing that they did not take place due to contamination. The methodological approach used in ancient DNA studies is therefore of great importance. In the study, the femur and talus bones were compared in terms of their suitability for this methodology. Samples with different levels of preservation required different applications in the pre-DNA preparation stage. As a result, no significant difference was found between the amount of DNA obtained from the two sample types and it was observed that the samples were successfully purified from modern human DNA contamination

Keywords

Ancient DNA, decontamination, DNA extraction, talus, femur

Farklı Koşullarda Bulunan Antik İskelet Örneklerinden Dekontaminasyon Süreci ile İlgili Metodolojik Yaklaşım

Abstract

Antik kalıntılardan DNA elde edilebileceğinin anlaşılması antropologların çalışmalarına yeni bir boyut kazandırmıştır. Bu önemli katkıya rağmen antik DNA analizleri, üzerinde çalışılan örneklerin doğası gereği birçok güçlüğü de beraberinde getirir. Bu çalışmalarda en önemli sorun yeterince korunmuş örnekleri seçmek ve bu örneklerden mümkün olan en kaliteli DNA’yı elde etmektir. Antik örnekler DNA eldesinden önce farklı aşamalarda maruz kaldıkları dış kontaminantlardan arındırılmalıdır. Arındırma sürecinin başarısı, alınacak sonuçların güvenirliliği açısından büyük önem taşır. Süreç sonunda alınan her sonuç araştırmacı tarafından kontaminasyon nedeni ile gerçekleşmediği gösterilerek savunulmalıdır. Antik DNA çalışmalarında kullanılan metodolojik yaklaşım bu nedenle büyük önem taşır. Çalışmada Femur ve Talus kemikleri bu metodolojiye uygunlukları açısından kıyaslanmıştır. Korunma düzeyleri farklı olan örnekler DNA eldesi öncesi hazırlık sürecinde farklı uygulamalar gerektirmiştir. Sonuç olarak iki örnek tipinden elde edilen DNA miktarı arasında belirgin bir fark bulunamamış ve örneklerin modern insan DNA’sı kontaminasyonundan arındırılabildiği gözlenmiştir

Keywords

Antik DNA, dekontaminasyon, DNA ekstraksiyonu, Talus, Femur

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