TR
EN
Culturability of rat bone marrow stromal cells and evaluation for osteoblastic formation
Abstract
Objectives: We investigated culturability of bone marrow stromal cells of young mature rats and evaluated cultured cells with regard to histomorphologic features and osteoblastic formation.
Methods: Sixteen mature Wistar rats were sacrificed under high dose of ether anesthesia. Their femurs and tibias were removed, medullas and metaphyses were washed with carrier serum and the collected material was cultured in DME/F12 serum. The cells in the test medium and at the bottom of Petri dishes were studied under inverted microscope and were photographed. Then, the cells at the bottom were stained with the picro-thionin technique, examined under light microscope, and photographed. Following trypsinizing, cluster cells were dissolved and smears were prepared for histomorphologic evaluation of stromal cells.
Results: Rat bone marrow cells in the culture showed a high percentage of reproducibility (93.8%; 15/16). The stromal cells in Petri dishes were of different morphology, and were interspersed with large multinucleated osteoblast-like cells following staining with the picrothionin technique.
Conclusion : Our findings favor the opinion that cells harvested in bone marrow cell culture are very similar to osteoblastic cells and that they may stem from osteoprogenitor cells which have multifacet differentionability.
Methods: Sixteen mature Wistar rats were sacrificed under high dose of ether anesthesia. Their femurs and tibias were removed, medullas and metaphyses were washed with carrier serum and the collected material was cultured in DME/F12 serum. The cells in the test medium and at the bottom of Petri dishes were studied under inverted microscope and were photographed. Then, the cells at the bottom were stained with the picro-thionin technique, examined under light microscope, and photographed. Following trypsinizing, cluster cells were dissolved and smears were prepared for histomorphologic evaluation of stromal cells.
Results: Rat bone marrow cells in the culture showed a high percentage of reproducibility (93.8%; 15/16). The stromal cells in Petri dishes were of different morphology, and were interspersed with large multinucleated osteoblast-like cells following staining with the picrothionin technique.
Conclusion : Our findings favor the opinion that cells harvested in bone marrow cell culture are very similar to osteoblastic cells and that they may stem from osteoprogenitor cells which have multifacet differentionability.
Keywords
Details
Primary Language
English
Subjects
Health Care Administration
Journal Section
Research Article
Publication Date
September 11, 2006
Submission Date
March 7, 2014
Acceptance Date
-
Published in Issue
Year 2006 Volume: 40 Number: 1
APA
Ceyhan, T., Bilir, A., & Karaca, C. (2006). Culturability of rat bone marrow stromal cells and evaluation for osteoblastic formation. Acta Orthopaedica et Traumatologica Turcica, 40(1), 67-71. https://izlik.org/JA63DF96GR
AMA
1.Ceyhan T, Bilir A, Karaca C. Culturability of rat bone marrow stromal cells and evaluation for osteoblastic formation. Acta Orthopaedica et Traumatologica Turcica. 2006;40(1):67-71. https://izlik.org/JA63DF96GR
Chicago
Ceyhan, Taskin, Ayhan Bilir, and Cetin Karaca. 2006. “Culturability of Rat Bone Marrow Stromal Cells and Evaluation for Osteoblastic Formation”. Acta Orthopaedica et Traumatologica Turcica 40 (1): 67-71. https://izlik.org/JA63DF96GR.
EndNote
Ceyhan T, Bilir A, Karaca C (September 1, 2006) Culturability of rat bone marrow stromal cells and evaluation for osteoblastic formation. Acta Orthopaedica et Traumatologica Turcica 40 1 67–71.
IEEE
[1]T. Ceyhan, A. Bilir, and C. Karaca, “Culturability of rat bone marrow stromal cells and evaluation for osteoblastic formation”, Acta Orthopaedica et Traumatologica Turcica, vol. 40, no. 1, pp. 67–71, Sept. 2006, [Online]. Available: https://izlik.org/JA63DF96GR
ISNAD
Ceyhan, Taskin - Bilir, Ayhan - Karaca, Cetin. “Culturability of Rat Bone Marrow Stromal Cells and Evaluation for Osteoblastic Formation”. Acta Orthopaedica et Traumatologica Turcica 40/1 (September 1, 2006): 67-71. https://izlik.org/JA63DF96GR.
JAMA
1.Ceyhan T, Bilir A, Karaca C. Culturability of rat bone marrow stromal cells and evaluation for osteoblastic formation. Acta Orthopaedica et Traumatologica Turcica. 2006;40:67–71.
MLA
Ceyhan, Taskin, et al. “Culturability of Rat Bone Marrow Stromal Cells and Evaluation for Osteoblastic Formation”. Acta Orthopaedica et Traumatologica Turcica, vol. 40, no. 1, Sept. 2006, pp. 67-71, https://izlik.org/JA63DF96GR.
Vancouver
1.Taskin Ceyhan, Ayhan Bilir, Cetin Karaca. Culturability of rat bone marrow stromal cells and evaluation for osteoblastic formation. Acta Orthopaedica et Traumatologica Turcica [Internet]. 2006 Sep. 1;40(1):67-71. Available from: https://izlik.org/JA63DF96GR