Düşük doz X ışınının hiyalin kıkırdak benzeri fibrokıkırdağın yapısal ve mekanik özellikleri üstündeki etkisi

Abstract

Amaç: Bu çalışmanın amacı düşük doz X ışınının fibröz kıkırdak üzerindeki etkilerini araştırmak ve hiyalin kıkırdak ile aynı mekanik ve yapısal özelliklere sahip hiyalin kıkırdak benzeri fibrokıkırdak (HKBF) geliştirmek idi. Çalışma planı: Yirmi tavşanın 40 diz ekleminde osteokondral defekt oluşturuldu. Ameliyat sonrası 7. günde başlanarak her tavşanın tek bir dizine, 5 gün boyunca, günlük 1.0 Gy'lik dozlarla toplam 5.0 Gy X ışını uygulanırken (radyoterapi grubu), diğer dizi uygulama dışı bırakıldı (kontrol grubu). Tavşanlar daha sonra her birinde 5 adet olacak şekilde 4 gruba ayrıldı. İlk üç grup, sırasıyla, 4., 8. ve 12. haftada sakrifiye edildi ve kıkırdak defektleri makroskopik ve mikroskobik olarak incelendi. Kalan 5 tavşanın oluşturduğu grup da 12. haftada sakrifiye edilerek kıkırdak defektlerine biyomekanik sıkıştırma testleri uygulandı. Bulgular: Radyoterapi ve kontrol grupları arasında biyomekanik açıdan anlamlı bir fark saptanmadı (p=0.686). Yine, gruplar arasında makroskopik ve mikroskopik açıdan da anlamlı bir fark yoktu (p=0.300). Işınlanan grupta kondrosit kümelenmesi gözlendi. Çıkarımlar: Düşük doz X ışını HKBF dokusunun mekanik özelliklerini in vivo etkilememekle birlikte, kondrosit kümelenmesi gibi yapısal değişikliklere yol açmaktadır.

Keywords

• Düşük doz X ışını; hiyalin kıkırdak; hiyalin kıkırdak benzeri fibrokıkırdak; mekanik; yapısal.

Effect of low-dose irradiation on structural and mechanical properties of hyaline cartilage-like fibrocartilage

Abstract

Objective: The aim of this study was to analyze the effect of low-dose irradiation on fibrous cartilage and to obtain a hyaline cartilage-like fibrocartilage (HCLF) with similar structural and mechanical properties to hyaline cartilage.
Methods: An osteochondral defect was created in 40 knees of 20 rabbits. At the 7th postoperative day, a single knee of each rabbit was irradiated with a total dose of 5.0 Gy in 1.0 Gy fractions for 5 days (radiotherapy group), while the other knee was not irradiated (control group). Rabbits were then divided into four groups of 5 rabbits each. The first three groups were sacrificed at the 4th, 8th and the 12th postoperative weeks and cartilage defects were macroscopically and microscopically evaluated. The remaining group of 5 rabbits was sacrificed at the 12th week and biomechanical compression tests were performed on the cartilage defects.
Results: There was no significant biomechanical difference between the radiotherapy and the control group (p=0.686). There was no significant macroscopic and microscopic difference between groups (p=0.300). Chondrocyte clustering was observed in the irradiated group.
Conclusion: Low-dose irradiation does not affect the mechanical properties of HCLF in vivo. However, structural changes such as chondrocyte clustering were observed.

Keywords

• Hyaline cartilage
• hyaline cartilage-like fibrocartilage
• low-dose irradiation
• mechanical
• structural

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