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Koenzim Q10 (CoQ10)’nun sıçanlarda steroide bağlı osteonekroz üzerine önleyici etkisi

Year 2014, , 217 - 222, 22.04.2014
https://doi.org/10.3944/AOTT.2014.13.0150

Abstract

Amaç: Bu çalışmanın amacı koenzim Q10 (CoQ10)’nun sıçanlarda steroide bağlı femur başı osteonekrozu (FBON) üzerindeki önleyici etkilerini incelemekti.

Çalışma planı: Osteonekrozu indüklemek için, 20 Sprague-Dawley cinsi sıçanın sağ gluteus medius kası içerisine bir kez 20 mg/kg metilprednizolon asetat enjekte edildi. Hayvanlar iki eşit gruba ayrıldı: 10 sıçandan oluşan Grup 1’e (kontrol grubu) profilaksi uygulanmazken, 10 sıçandan oluşan Grup 2’ye CoQ10 verildi. Steroid enjeksiyonu yapılmadan hemen önce (0. Hafta) ve steroid enjeksiyonundan 4 hafta sonra hematolojik incelemeler yapıldı. Osteonekrozu değerlendirmek amacıyla femur başları histolojik olarak incelendi.

Bulgular: Kandaki glutatyon (GSH) ve malondialdehit (MDA) konsantrasyonlarına dair değişiklikler CoQ10 grubunda daha az belirgin idi. CoQ10 grubunda erken osteonekroz ile uyumlu histolojik değişikliklerin görülme sıklığı (10 sıçandan 2’si; %20) kontrol grubuna (10 sıçandan 7’si; %70) kıyasla daha düşüktü.

Çıkarımlar: Koenzim Q10 steroide bağlı FBON’yi önleyici bir ajan olarak yararlı olabilir. Oksidatif stresin inhibe edilmesi bu etkiyi açıklayabilecek olası bir mekanizmadır.

References

  • Mont MA, Jones LC, Hungerford DS. Nontraumatic osteonecrosis of the femoral head: ten years later. J Bone Joint Surg Am 2006;88:1117-32.
  • Kuribayashi M, Fujioka M, Takahashi KA, Arai Y, Ishida M, Goto T, et al. Vitamin E prevents steroid-induced osteonecrosis in rabbits. Acta Orthop 2010;81:154-60.
  • Lu BB, Li KH. Lipoic acid prevents steroid-induced osteonecrosis in rabbits. Rheumatol Int 2012;32:1679-83.
  • Nagasawa K, Tada Y, Koarada S, Tsukamoto H, Horiuchi T, Yoshizawa S, et al. Prevention of steroid-induced osteonecrosis of femoral head in systemic lupus erythematosus by anti-coagulant. Lupus 2006;15:354-7.
  • Mont MA, Hungerford DS. Non-traumatic avascular necrosis of the femoral head. J Bone Joint Surg Am 1995;77:459-74.
  • Ichiseki T, Kaneuji A, Katsuda S, Ueda Y, Sugimori T, Matsumoto T. DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis. Rheumatology (Oxford) 2005;44:456-60.
  • Ichiseki T, Matsumoto T, Nishino M, Kaneuji A, Katsuda S. Oxidative stress and vascular permeability in steroidinduced osteonecrosis model. J Orthop Sci 2004;9:509
  • Overvad K, Diamant B, Holm L, Holmer G, Mortensen SA, Stender S. Coenzyme Q10 in health and disease. Eur J Clin Nutr 1999;53:764-70.
  • Naderi J, Somayajulu-Nitu M, Mukerji A, Sharda P, Sikorska M, Borowy-Borowski H, et al. Water-soluble formulation of Coenzyme Q10 inhibits Bax-induced destabilization of mitochondria in mammalian cells. Apoptosis 2006;11:1359-69.
  • Yamamoto T, Irisa T, Sugioka Y, Sueishi K. Effects of pulse methylprednisolone on bone and marrow tissues: corticosteroid-induced osteonecrosis in rabbits. Arthritis Rheum 1997;40:2055-64.
  • Binukumar BK, Gupta N, Bal A, Gill KD. Protection of dichlorvos induced oxidative stress and nigrostriatal neuronal death by chronic coenzyme Q10 pretreatment. Toxicol Appl Pharmacol 2011;256:73-82.
  • Farswan M, Rathod SP, Upaganlawar AB, Semwal A. Protective effect of coenzyme Q10 in simvastatin and gemfibrozil induced rhabdomyolysis in rats. Indian J Exp Biol 2005;43:845-8.
  • Upaganlawar A, Farswan M, Rathod S, Balaraman R. Modification of biochemical parameters of gentamicin nephrotoxicity by coenzyme Q10 and green tea in rats. Indian J Exp Biol 2006;44:416-8.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882-8.
  • Ding S, Peng H, Fang HS, Zhou JL, Wang Z. Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats. BMC Musculoskelet Disord 2011;12:215.
  • Kabata T, Kubo T, Matsumoto T, Nishino M, Tomita K, Katsuda S, et al. Apoptotic cell death in steroid induced osteonecrosis: an experimental study in rabbits. J Rheumatol 2000;27:2166-71.
  • Dallner G, Sindelar PJ. Regulation of ubiquinone metabolism. Free Radic Biol Med 2000;29:285-94.
  • Erol B, Bozlu M, Hanci V, Tokgoz H, Bektas S, Mungan G. Coenzyme Q10 treatment reduces lipid peroxidation, inducible and endothelial nitric oxide synthases, and germ cell-specific apoptosis in a rat model of testicular ischemia/reperfusion injury. Fertil Steril 2010;93:280-2.
  • Kerimoğlu A, Paşaoğlu O, Kanbak G, Hanci V, Ozdemir F, Atasoy MA. Efficiency of coenzyme Q(10) at experimental spinal cord injury. Ulus Travma Acil Cerrahi Derg 2007;13:85-93.
  • Yokoyama K, Itoman M, Takagishi K, Yamamoto M. Protective effects of coenzyme Q10 on ischemia-induced reperfusion injury in ischemic limb models. Plast Reconstr Surg 1992;90:890-8.
  • Mohammadi S, Najafi M, Hamzeiy H, Maleki-Dizaji N, Pezeshkian M, Sadeghi-Bazargani H, et al. Protective effects of methylsulfonylmethane on hemodynamics and oxidative stress in monocrotaline-induced pulmonary hypertensive rats. Adv Pharmacol Sci 2012;2012:507278.
  • Yenilmez A, Isikli B, Aral E, Degirmenci I, Sutken E, Baycu C. Antioxidant effects of melatonin and coenzyme Q10 on oxidative damage caused by single-dose ochratoxin A in rat kidney. Chin J Physiol 2010;53:310-7.
  • Ostrowski RP. Effect of coenzyme Q(10) on biochemical and morphological changes in experimental ischemia in the rat brain. Brain Res Bull 2000;53:399-407.
  • Miyanishi K, Yamamoto T, Irisa T, Yamashita A, Jingushi S, Noguchi Y, et al. A high low-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio as a potential risk factor for corticosteroid-induced osteonecrosis in rabbits. Rheumatology (Oxford) 2001;40:196-201.
  • Motomura G, Yamamoto T, Miyanishi K, Jingushi S, Iwamoto Y. Combined effects of an anticoagulant and a lipid-lowering agent on the prevention of steroid-induced osteonecrosis in rabbits. Arthritis Rheum 2004;50:3387

Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats

Year 2014, , 217 - 222, 22.04.2014
https://doi.org/10.3944/AOTT.2014.13.0150

Abstract

Objective: The aim of this study was to examine the role of coenzyme Q10 (CoQ10) in the prevention of steroid-induced osteonecrosis of the femoral head (ONFH) in rats.

Methods: The study included 20 Sprague-Dawley rats injected once with 20 mg/kg of methylprednisolone acetate into the right gluteus medius muscle to induce osteonecrosis. Animals were divided into two equal groups; Group 1 received no prophylaxis (control group) and the Group 2 received CoQ10. Hematological examinations were performed before steroid injection (0 weeks) and at 4 weeks after steroid injection. Femoral heads were examined histologically to evaluate osteonecrosis.

Results: Changes in blood glutathione (GSH) and malondialdehyde (MDA) concentrations were less significant in the CoQ10 group. The incidence of histologic changes consistent with early osteonecrosis was lower in the CoQ10 group (2 of 10; 20%) than the control group (7 of 10; 70%).

Conclusion: Coenzyme Q10 may be useful as a preventing agent in steroid-induced ONFH. Inhibited oxidative stress is a possible mechanism for this effect.

References

  • Mont MA, Jones LC, Hungerford DS. Nontraumatic osteonecrosis of the femoral head: ten years later. J Bone Joint Surg Am 2006;88:1117-32.
  • Kuribayashi M, Fujioka M, Takahashi KA, Arai Y, Ishida M, Goto T, et al. Vitamin E prevents steroid-induced osteonecrosis in rabbits. Acta Orthop 2010;81:154-60.
  • Lu BB, Li KH. Lipoic acid prevents steroid-induced osteonecrosis in rabbits. Rheumatol Int 2012;32:1679-83.
  • Nagasawa K, Tada Y, Koarada S, Tsukamoto H, Horiuchi T, Yoshizawa S, et al. Prevention of steroid-induced osteonecrosis of femoral head in systemic lupus erythematosus by anti-coagulant. Lupus 2006;15:354-7.
  • Mont MA, Hungerford DS. Non-traumatic avascular necrosis of the femoral head. J Bone Joint Surg Am 1995;77:459-74.
  • Ichiseki T, Kaneuji A, Katsuda S, Ueda Y, Sugimori T, Matsumoto T. DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis. Rheumatology (Oxford) 2005;44:456-60.
  • Ichiseki T, Matsumoto T, Nishino M, Kaneuji A, Katsuda S. Oxidative stress and vascular permeability in steroidinduced osteonecrosis model. J Orthop Sci 2004;9:509
  • Overvad K, Diamant B, Holm L, Holmer G, Mortensen SA, Stender S. Coenzyme Q10 in health and disease. Eur J Clin Nutr 1999;53:764-70.
  • Naderi J, Somayajulu-Nitu M, Mukerji A, Sharda P, Sikorska M, Borowy-Borowski H, et al. Water-soluble formulation of Coenzyme Q10 inhibits Bax-induced destabilization of mitochondria in mammalian cells. Apoptosis 2006;11:1359-69.
  • Yamamoto T, Irisa T, Sugioka Y, Sueishi K. Effects of pulse methylprednisolone on bone and marrow tissues: corticosteroid-induced osteonecrosis in rabbits. Arthritis Rheum 1997;40:2055-64.
  • Binukumar BK, Gupta N, Bal A, Gill KD. Protection of dichlorvos induced oxidative stress and nigrostriatal neuronal death by chronic coenzyme Q10 pretreatment. Toxicol Appl Pharmacol 2011;256:73-82.
  • Farswan M, Rathod SP, Upaganlawar AB, Semwal A. Protective effect of coenzyme Q10 in simvastatin and gemfibrozil induced rhabdomyolysis in rats. Indian J Exp Biol 2005;43:845-8.
  • Upaganlawar A, Farswan M, Rathod S, Balaraman R. Modification of biochemical parameters of gentamicin nephrotoxicity by coenzyme Q10 and green tea in rats. Indian J Exp Biol 2006;44:416-8.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882-8.
  • Ding S, Peng H, Fang HS, Zhou JL, Wang Z. Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats. BMC Musculoskelet Disord 2011;12:215.
  • Kabata T, Kubo T, Matsumoto T, Nishino M, Tomita K, Katsuda S, et al. Apoptotic cell death in steroid induced osteonecrosis: an experimental study in rabbits. J Rheumatol 2000;27:2166-71.
  • Dallner G, Sindelar PJ. Regulation of ubiquinone metabolism. Free Radic Biol Med 2000;29:285-94.
  • Erol B, Bozlu M, Hanci V, Tokgoz H, Bektas S, Mungan G. Coenzyme Q10 treatment reduces lipid peroxidation, inducible and endothelial nitric oxide synthases, and germ cell-specific apoptosis in a rat model of testicular ischemia/reperfusion injury. Fertil Steril 2010;93:280-2.
  • Kerimoğlu A, Paşaoğlu O, Kanbak G, Hanci V, Ozdemir F, Atasoy MA. Efficiency of coenzyme Q(10) at experimental spinal cord injury. Ulus Travma Acil Cerrahi Derg 2007;13:85-93.
  • Yokoyama K, Itoman M, Takagishi K, Yamamoto M. Protective effects of coenzyme Q10 on ischemia-induced reperfusion injury in ischemic limb models. Plast Reconstr Surg 1992;90:890-8.
  • Mohammadi S, Najafi M, Hamzeiy H, Maleki-Dizaji N, Pezeshkian M, Sadeghi-Bazargani H, et al. Protective effects of methylsulfonylmethane on hemodynamics and oxidative stress in monocrotaline-induced pulmonary hypertensive rats. Adv Pharmacol Sci 2012;2012:507278.
  • Yenilmez A, Isikli B, Aral E, Degirmenci I, Sutken E, Baycu C. Antioxidant effects of melatonin and coenzyme Q10 on oxidative damage caused by single-dose ochratoxin A in rat kidney. Chin J Physiol 2010;53:310-7.
  • Ostrowski RP. Effect of coenzyme Q(10) on biochemical and morphological changes in experimental ischemia in the rat brain. Brain Res Bull 2000;53:399-407.
  • Miyanishi K, Yamamoto T, Irisa T, Yamashita A, Jingushi S, Noguchi Y, et al. A high low-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio as a potential risk factor for corticosteroid-induced osteonecrosis in rabbits. Rheumatology (Oxford) 2001;40:196-201.
  • Motomura G, Yamamoto T, Miyanishi K, Jingushi S, Iwamoto Y. Combined effects of an anticoagulant and a lipid-lowering agent on the prevention of steroid-induced osteonecrosis in rabbits. Arthritis Rheum 2004;50:3387
There are 26 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Experimental Study
Authors

Erkam Komurcu This is me

Murat Oktay This is me

Burak Kaymaz This is me

Umut Hatay Golge This is me

Ferdi Goksel This is me

Gurdal Nusran This is me

Publication Date April 22, 2014
Published in Issue Year 2014

Cite

APA Komurcu, E., Oktay, M., Kaymaz, B., Hatay Golge, U., et al. (2014). Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats. Acta Orthopaedica Et Traumatologica Turcica, 48(2), 217-222. https://doi.org/10.3944/AOTT.2014.13.0150
AMA Komurcu E, Oktay M, Kaymaz B, Hatay Golge U, Goksel F, Nusran G. Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats. Acta Orthopaedica et Traumatologica Turcica. April 2014;48(2):217-222. doi:10.3944/AOTT.2014.13.0150
Chicago Komurcu, Erkam, Murat Oktay, Burak Kaymaz, Umut Hatay Golge, Ferdi Goksel, and Gurdal Nusran. “Preventive Effects of Coenzyme Q10 (CoQ10) on Steroid-Induced Osteonecrosis in Rats”. Acta Orthopaedica Et Traumatologica Turcica 48, no. 2 (April 2014): 217-22. https://doi.org/10.3944/AOTT.2014.13.0150.
EndNote Komurcu E, Oktay M, Kaymaz B, Hatay Golge U, Goksel F, Nusran G (April 1, 2014) Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats. Acta Orthopaedica et Traumatologica Turcica 48 2 217–222.
IEEE E. Komurcu, M. Oktay, B. Kaymaz, U. Hatay Golge, F. Goksel, and G. Nusran, “Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats”, Acta Orthopaedica et Traumatologica Turcica, vol. 48, no. 2, pp. 217–222, 2014, doi: 10.3944/AOTT.2014.13.0150.
ISNAD Komurcu, Erkam et al. “Preventive Effects of Coenzyme Q10 (CoQ10) on Steroid-Induced Osteonecrosis in Rats”. Acta Orthopaedica et Traumatologica Turcica 48/2 (April 2014), 217-222. https://doi.org/10.3944/AOTT.2014.13.0150.
JAMA Komurcu E, Oktay M, Kaymaz B, Hatay Golge U, Goksel F, Nusran G. Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats. Acta Orthopaedica et Traumatologica Turcica. 2014;48:217–222.
MLA Komurcu, Erkam et al. “Preventive Effects of Coenzyme Q10 (CoQ10) on Steroid-Induced Osteonecrosis in Rats”. Acta Orthopaedica Et Traumatologica Turcica, vol. 48, no. 2, 2014, pp. 217-22, doi:10.3944/AOTT.2014.13.0150.
Vancouver Komurcu E, Oktay M, Kaymaz B, Hatay Golge U, Goksel F, Nusran G. Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats. Acta Orthopaedica et Traumatologica Turcica. 2014;48(2):217-22.