Koenzim Q10 (CoQ10)’nun sıçanlarda steroide bağlı osteonekroz üzerine önleyici etkisi

Öz

Amaç: Bu çalışmanın amacı koenzim Q10 (CoQ10)’nun sıçanlarda steroide bağlı femur başı osteonekrozu (FBON) üzerindeki önleyici etkilerini incelemekti.

Çalışma planı: Osteonekrozu indüklemek için, 20 Sprague-Dawley cinsi sıçanın sağ gluteus medius kası içerisine bir kez 20 mg/kg metilprednizolon asetat enjekte edildi. Hayvanlar iki eşit gruba ayrıldı: 10 sıçandan oluşan Grup 1’e (kontrol grubu) profilaksi uygulanmazken, 10 sıçandan oluşan Grup 2’ye CoQ10 verildi. Steroid enjeksiyonu yapılmadan hemen önce (0. Hafta) ve steroid enjeksiyonundan 4 hafta sonra hematolojik incelemeler yapıldı. Osteonekrozu değerlendirmek amacıyla femur başları histolojik olarak incelendi.

Bulgular: Kandaki glutatyon (GSH) ve malondialdehit (MDA) konsantrasyonlarına dair değişiklikler CoQ10 grubunda daha az belirgin idi. CoQ10 grubunda erken osteonekroz ile uyumlu histolojik değişikliklerin görülme sıklığı (10 sıçandan 2’si; %20) kontrol grubuna (10 sıçandan 7’si; %70) kıyasla daha düşüktü.

Çıkarımlar: Koenzim Q10 steroide bağlı FBON’yi önleyici bir ajan olarak yararlı olabilir. Oksidatif stresin inhibe edilmesi bu etkiyi açıklayabilecek olası bir mekanizmadır.

Anahtar Kelimeler

• Glutatyon; koenzim Q10; malondialdehit; steroide bağlı osteonekroz.

Preventive effects of coenzyme Q10 (CoQ10) on steroid-induced osteonecrosis in rats

Abstract

Objective: The aim of this study was to examine the role of coenzyme Q10 (CoQ10) in the prevention of steroid-induced osteonecrosis of the femoral head (ONFH) in rats.

Methods: The study included 20 Sprague-Dawley rats injected once with 20 mg/kg of methylprednisolone acetate into the right gluteus medius muscle to induce osteonecrosis. Animals were divided into two equal groups; Group 1 received no prophylaxis (control group) and the Group 2 received CoQ10. Hematological examinations were performed before steroid injection (0 weeks) and at 4 weeks after steroid injection. Femoral heads were examined histologically to evaluate osteonecrosis.

Results: Changes in blood glutathione (GSH) and malondialdehyde (MDA) concentrations were less significant in the CoQ10 group. The incidence of histologic changes consistent with early osteonecrosis was lower in the CoQ10 group (2 of 10; 20%) than the control group (7 of 10; 70%).

Conclusion: Coenzyme Q10 may be useful as a preventing agent in steroid-induced ONFH. Inhibited oxidative stress is a possible mechanism for this effect.

Keywords

• Coenzyme Q10
• glutathione
• malondialdehyde
• steroid-induced osteonecrosis

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