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İmmobilizasyon ve denervasyon atrofilerinin histomorfolojik karşılaştırması

Year 2014, , 320 - 325, 30.05.2014
https://doi.org/10.3944/AOTT.2014.2993

Abstract

Amaç:Bu çalışmada oluşturulan deneysel modelde immobilizasyon ve denervasyon atrofileri sonrası kas dokusundaki histomorfolojik değişimlerin karşılaştırılması amaçlandı.

Çalışma planı: Ross-800 cinsi 30 adet melez piliç (60 ayak) 2 çalışma (immobilizasyon ve denervasyon) ve 2 kontrol grubuna ayrıldı. İmmobilizasyon atrofisi grubundaki tavukların diz ve ayak bileği eklemleri Kirschner teli ile tespit edildi. Denervasyon grubu için siyatik sinir rezeksiyonu yapıldı. Her iki grubun sağlam taraflarındaki ayaklar kontrol gruplarını oluşturdular. Üç hafta sonunda iki grubun ağırlıkları, hacimleri, yükseklikleri, Aşil tendonlarının çap ve uzama miktarları, yağlanma, dejenerasyon değişim ve fibrozis miktarları karşılaştırıldı. Kasların histopatolojik değerlendirmesi için hematoksilen-eozin boyama yapıldı. Karşılaştırmalarda Mann-Whitney U-testinden yararlanıldı.

Bulgular: Denervasyon grubundaki hacim, ağırlık ve kas uzunluk kayıpları immobilizasyon grubuna göre anlamlı derecede daha düşüktü (p<0.05). Aşil tendon çapları ve uzunlukları ile kısa baş çapları arasındaki farklar istatistiksel olarak anlamlı değildi. Denervasyon grubu ile immobilizasyon grubu arasında yağlanma, dejenerasyon, fibrozis açısından istatistiksel olarak anlamlı farklılık vardı (p<0.05). Noktacık sayma yöntemindeki bulgularımız, denervasyon grubundaki yağ dokusu alanlarındaki (beyaz alan) noktacık sayıları ile immobilizasyon grubu arasında anlamlı farka işaret etmekteydi (p<0.05).

Çıkarımlar: Deneysel piliç modelindeki çalışma sonuçlarımız denervasyon grubundaki histomorfolojik değişimlerin immobilizasyon grubuna göre daha fazla olduğunu göstermiştir.

References

  • Busquets S, Alvarez B, López-Soriano FJ, Argilés JM. Branched-chain amino acids: a role in skeletal muscle pro- teolysis in catabolic states? J Cell Physiol 2002;191:283-9.
  • Bloomfield SA. Changes in musculoskeletal structure and function with prolonged bed rest. Med Sci Sports Exerc 1997;29:197-206. CrossRef
  • Powers SK, Wiggs MP, Duarte JA, Zergeroglu AM, Demirel HA. Mitochondrial signaling contributes to disuse muscle atrophy. Am J Physiol Endocrinol Metab 2012;303:E31-9. CrossRef
  • Buege JA, Aust SD. Microsomal lipid peroxidation. Meth- ods Enzymol 1978;52:302-10. CrossRef
  • Zarzhevsky N, Coleman R, Volpin G, Fuchs D, Stein H, Reznick AZ. Muscle recovery after immobilisation by ex- ternal fixation. J Bone Joint Surg Br 1999;81:896-901.
  • Coutinho EL, Gomes AR, França CN, Oishi J, Sal- vini TF. Effect of passive stretching on the immobilized soleus muscle fiber morphology. Braz J Med Biol Res 2004;37:1853-61. CrossRef
  • Tate CA, Bick RJ, Myers TD, Pitts BJ, Van Winkle WB, Entman ML. Alteration of sarcoplasmic reticulum af- ter denervation of chicken pectoralis muscle. Biochem J 1983;210:339-44.
  • Tomanek RJ, Lund DD. Degeneration of different types of skeletal muscle fibres. I. Denervation. J Anat 1973;116:395-407.
  • Camillo AC, Rocha Rde C, Chopard RP. Structural and microvascular study of soleous muscle of Wistar rats after section of the sciatic nerve. Arq Neuropsiquiatr 2004;62:835-8. CrossRef
  • Sunderland S, Ray LJ. Denervation changes in mam- malian striated muscle. J Neurol Neurosurg Psychiatry 1950;13:159-77. CrossRef
  • Tanaka T, Kariya Y, Hoshino Y. Histochemical study on the changes in muscle fibers in relation to the effects of aging on recovery from muscular atrophy caused by disuse in rats. J Orthop Sci 2004;9:76-85. CrossRef
  • Tabary JC, Tabary C, Tardieu C, Tardieu G, Goldspink G. Physiological and structural changes in the cat’s soleus muscle due to immobilization at different lengths by plas- ter casts. J Physiol 1972;224:231-44.
  • Toraman S, Türkoğlu I. Automatic identification of select- ed cell areas in histopathological image. 5th International Advanced Technologies Symposium, May 13-15, 2009. Karabük, Türkiye.

Histomorphological comparison of immobilization and denervation atrophies

Year 2014, , 320 - 325, 30.05.2014
https://doi.org/10.3944/AOTT.2014.2993

Abstract

Objective:The aim of this study was to compare the histomorphological changes in the muscle tissue after immobilization and denervation atrophies in an animal model.

Methods: The study included 30 Ross-800 hybrid chickens (60 legs) divided into two study (immobilization and denervation) and two control groups. The knee and ankle joints were fixed with a Kirschner wire in the immobilization atrophy group and sciatic nerve resection was performed in the denervation group. The unaffected side of each group was used as controls. The weight, volume, height, diameter and the rate of elongation of the Achilles tendons, and the amount of fat deposition, degeneration and fibrosis were compared between the two groups at the end of 3 weeks. Hematoxylin-eosin staining was performed for a histopathological assessment of the muscles. The Mann-Whitney U-test was used for comparisons.

Results: Loss of the volume, weight and muscle length was significantly lower in the denervation group than the immobilization group (p<0.05). Differences between the diameter of the Achilles tendon and length and diameter of the short heads were not statistically significant. There were statistically significant differences in fat deposition, degeneration and fibrosis between the degeneration group and the immobilization group (p<0.05). Pixel counting revealed a significant difference in the number of pixels in the fatty tissue area (white area) between the denervation group and the immobilization group (p<0.05).

Conclusion: Our results showed that histomorphological changes were more in the denervation group than the immobilization group in an experimental chicken model.

References

  • Busquets S, Alvarez B, López-Soriano FJ, Argilés JM. Branched-chain amino acids: a role in skeletal muscle pro- teolysis in catabolic states? J Cell Physiol 2002;191:283-9.
  • Bloomfield SA. Changes in musculoskeletal structure and function with prolonged bed rest. Med Sci Sports Exerc 1997;29:197-206. CrossRef
  • Powers SK, Wiggs MP, Duarte JA, Zergeroglu AM, Demirel HA. Mitochondrial signaling contributes to disuse muscle atrophy. Am J Physiol Endocrinol Metab 2012;303:E31-9. CrossRef
  • Buege JA, Aust SD. Microsomal lipid peroxidation. Meth- ods Enzymol 1978;52:302-10. CrossRef
  • Zarzhevsky N, Coleman R, Volpin G, Fuchs D, Stein H, Reznick AZ. Muscle recovery after immobilisation by ex- ternal fixation. J Bone Joint Surg Br 1999;81:896-901.
  • Coutinho EL, Gomes AR, França CN, Oishi J, Sal- vini TF. Effect of passive stretching on the immobilized soleus muscle fiber morphology. Braz J Med Biol Res 2004;37:1853-61. CrossRef
  • Tate CA, Bick RJ, Myers TD, Pitts BJ, Van Winkle WB, Entman ML. Alteration of sarcoplasmic reticulum af- ter denervation of chicken pectoralis muscle. Biochem J 1983;210:339-44.
  • Tomanek RJ, Lund DD. Degeneration of different types of skeletal muscle fibres. I. Denervation. J Anat 1973;116:395-407.
  • Camillo AC, Rocha Rde C, Chopard RP. Structural and microvascular study of soleous muscle of Wistar rats after section of the sciatic nerve. Arq Neuropsiquiatr 2004;62:835-8. CrossRef
  • Sunderland S, Ray LJ. Denervation changes in mam- malian striated muscle. J Neurol Neurosurg Psychiatry 1950;13:159-77. CrossRef
  • Tanaka T, Kariya Y, Hoshino Y. Histochemical study on the changes in muscle fibers in relation to the effects of aging on recovery from muscular atrophy caused by disuse in rats. J Orthop Sci 2004;9:76-85. CrossRef
  • Tabary JC, Tabary C, Tardieu C, Tardieu G, Goldspink G. Physiological and structural changes in the cat’s soleus muscle due to immobilization at different lengths by plas- ter casts. J Physiol 1972;224:231-44.
  • Toraman S, Türkoğlu I. Automatic identification of select- ed cell areas in histopathological image. 5th International Advanced Technologies Symposium, May 13-15, 2009. Karabük, Türkiye.
There are 13 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Experimental Study
Authors

Onder Ceylan This is me

Firat Seyfettinoglu This is me

Ali Murat Dulgeroglu This is me

Arzu Avci This is me

Bulent Bayram This is me

Osman Arslan Bora This is me

Publication Date May 30, 2014
Published in Issue Year 2014

Cite

APA Ceylan, O., Seyfettinoglu, F., Murat Dulgeroglu, A., Avci, A., et al. (2014). Histomorphological comparison of immobilization and denervation atrophies. Acta Orthopaedica Et Traumatologica Turcica, 48(3), 320-325. https://doi.org/10.3944/AOTT.2014.2993
AMA Ceylan O, Seyfettinoglu F, Murat Dulgeroglu A, Avci A, Bayram B, Arslan Bora O. Histomorphological comparison of immobilization and denervation atrophies. Acta Orthopaedica et Traumatologica Turcica. May 2014;48(3):320-325. doi:10.3944/AOTT.2014.2993
Chicago Ceylan, Onder, Firat Seyfettinoglu, Ali Murat Dulgeroglu, Arzu Avci, Bulent Bayram, and Osman Arslan Bora. “Histomorphological Comparison of Immobilization and Denervation Atrophies”. Acta Orthopaedica Et Traumatologica Turcica 48, no. 3 (May 2014): 320-25. https://doi.org/10.3944/AOTT.2014.2993.
EndNote Ceylan O, Seyfettinoglu F, Murat Dulgeroglu A, Avci A, Bayram B, Arslan Bora O (May 1, 2014) Histomorphological comparison of immobilization and denervation atrophies. Acta Orthopaedica et Traumatologica Turcica 48 3 320–325.
IEEE O. Ceylan, F. Seyfettinoglu, A. Murat Dulgeroglu, A. Avci, B. Bayram, and O. Arslan Bora, “Histomorphological comparison of immobilization and denervation atrophies”, Acta Orthopaedica et Traumatologica Turcica, vol. 48, no. 3, pp. 320–325, 2014, doi: 10.3944/AOTT.2014.2993.
ISNAD Ceylan, Onder et al. “Histomorphological Comparison of Immobilization and Denervation Atrophies”. Acta Orthopaedica et Traumatologica Turcica 48/3 (May 2014), 320-325. https://doi.org/10.3944/AOTT.2014.2993.
JAMA Ceylan O, Seyfettinoglu F, Murat Dulgeroglu A, Avci A, Bayram B, Arslan Bora O. Histomorphological comparison of immobilization and denervation atrophies. Acta Orthopaedica et Traumatologica Turcica. 2014;48:320–325.
MLA Ceylan, Onder et al. “Histomorphological Comparison of Immobilization and Denervation Atrophies”. Acta Orthopaedica Et Traumatologica Turcica, vol. 48, no. 3, 2014, pp. 320-5, doi:10.3944/AOTT.2014.2993.
Vancouver Ceylan O, Seyfettinoglu F, Murat Dulgeroglu A, Avci A, Bayram B, Arslan Bora O. Histomorphological comparison of immobilization and denervation atrophies. Acta Orthopaedica et Traumatologica Turcica. 2014;48(3):320-5.