The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis

Year 2013, Volume: 47 Issue: 4, 266 - 272, 18.09.2013

Bekir Cinar Esra Circi Cenk Balcik Gulnur Guven Sercan Akpinar Alihan Derincek

Abstract

Objective: The aim of this study was to investigate the influence of low-dose extracorporeal shock waves (ESW) on the healing potential of Achilles tendinitis in the rat.
Methods: The 36 adult Sprague-Dawley rats used in this study were randomly divided into four groups. Group A (n=10) were injected with carrageenan, Group B (n=10) were injected with carrageenan and received ESW, Group C (n=10) received ESW only, and Group D (n=6) was a sham group. Rats were injected with 10 microliters of 3% carrageenan or a saline solution eight times during a one-week period with a subcutaneous needle. One week following the final injection, ESW was applied at a rate of 500 impulses in 5 minutes at 2 bars (comparative to 0.09 mJ/mm2) to rats in Groups B and C. Rats were sacrificed three weeks later. Tensile strength, inflammation, and vascularity and collagen density were measured.
Results: Failure of the tendon ultimate loads was significantly lower in the study groups than in the control group (p<0.05). Collagen fiber density was higher in the control group than in the other groups (p=0.59). No other histological differences were found.
Conclusion: Low-dose ESW has a negative effect on tendon tensile strength in this animal model.

Keywords

Achilles tendinitis, biomechanical analysis, extracorporeal shock wave, histological analysis, rat

Sıçanlarda karragenan ile uyarılmış Aşil tendinitinde ekstrakorporeal şok dalgalarının etkinliği: Biyomekanik ve histolojik bir değerlendirme

Abstract

Amaç: Bu çalışmanın amacı sıçanlarda Aşil tendinitinin iyileşmesinde düşük doz ekstrakorporeal şok dalgaların etkinliğinin incelenmesi idi.
Çalışma planı: Çalışmada kullanılan 36 adet Sprague-Dawley sıçanı dört gruba ayrıldı. Grup A, karragenan enjekte edilen 10 sıçan, Grup B karragenan ve ekstrakorporeal şok dalga uygulanan 10 sıçan, Grup C ekstrakorporeal şok dalga uygulanan 10 sıçan ve Grup D kontrol grubu olarak 6 sıçandan oluşmaktaydı. Sıçanlara 10 mikrolitre %3’lük karragenan veya salin çözeltisi bir hafta içinde 8 defa subkutan iğne ile uygulandı. Son enjeksiyondan bir hafta sonra Grup B ve Grup C’deki sıçanlara 500 atım şok dalga 5 dakika süre ile 2 bar (yaklaşık 0.09 mJ/mm2) düzeyinde uygulandı. Sıçanlar üç hafta sonra sakrifiye edildi. Tendon gerim direnci, enflamasyon ve damarlanma ve kolajen yoğunlukları ölçüldü.
Bulgular: Çalışma gruplarındaki tendon gerim direncinin kontrol grubuna göre anlamlı düzeyde daha düşük olduğu belirlendi (p<0.05). Kolajen liflerin yoğunluğu kontrol grubunda diğer gruplardan daha yüksekti (p=0.59). Histolojik başka bir farklılık belirlenmedi.
Çıkarımlar: Bu hayvan modelinde düşük doz ekstrakorporeal şok dalgasının tendon gerim direncine olumsuz etkisi olduğu görülmüştür.

Keywords

Aşil tendiniti; biyomekanik inceleme; ekstrakorporeal şok dalga; histolojik inceleme; sıçan

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