Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle

Kenan Koca; Yuksel Yurttas; Cemil Yildiz; Tuncer Cayci; Bulent Uysal; Ahmet Korkmaz

Research Article

Year 2010, Volume: 44 Issue: 6, 476 - 483, 07.02.2011

Kenan Koca Yuksel Yurttas Cemil Yildiz Tuncer Cayci Bulent Uysal Ahmet Korkmaz

Abstract

Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle

Year 2010, Volume: 44 Issue: 6, 476 - 483, 07.02.2011

Kenan Koca Yuksel Yurttas Cemil Yildiz Tuncer Cayci Bulent Uysal Ahmet Korkmaz

Abstract

Objectives: The aim of the study was to investigate the effect of hyperbaric oxygen-preconditioning (HBO-PC) and ozone-preconditioning (O3-PC) on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion (I/R) in rat skeletal muscle.
Methods: Thirty-two Wistar-Albino-type male rats included in the study were divided into four groups of equal number: 1) sham operation, 2) I/R, 3) I/R+HBO-PC, or 4) I/R+O3-PC. One session of 3-4 L/min 100% oxygenation for 60 min at 3 absolute atmosphere (ATA) was defined as one dose of HBO; in total, 7 doses of HBO-PC were administered before ischemia. One dose of O3 comprised 0.7 mg/kg ozone/oxygen mixture, administered intraperitoneally; a total of 4 doses of O3-PC were administered before ischemia. The I/R model was performed in anesthetized rats by clipping right femoral artery to induce 2 h ischemia followed by 22 h of reperfusion. The right gastrocnemius muscle and venous blood samples were harvested. Tissue was assayed for levels of malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). Serum was assayed to measure the level of nitrite-nitrate (NOx).
Results: Tissue MDA level, SOD activity, and serum NOx level were increased, whereas GSHPx was decreased in the I/R group. MDA and NOx levels were decreased, whereas GSH-Px activity was increased in both the I/R+HBO-PC and I/R+O3-PC groups. SOD activity was increased in the I/R+O3-PC group, but did not change significantly in the I/R+HBO-PC group. iNOS staining score and intensity were lower in the I/R+HBO-PC and I/R+O3-PC groups than I/R group.
Conclusion: Both O3-PC and HBO-PC reduced tissue lipid peroxidation, NOx levels, and iNOS staining scores in the experimental I/R model. Our data suggest that HBO-PC and O3-PC protect against oxidative/nitrosative stress induced by I/R in rat skeletal muscle.

Keywords

Ischemia-reperfusion injury, hyperbaric oxygenation, oxidative stress, ozone, rats

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Details

Primary Language	English
Subjects	Health Care Administration
Journal Section	Experimental Study
Authors	Kenan Koca This is me Yuksel Yurttas This is me Cemil Yildiz This is me Tuncer Cayci This is me Bulent Uysal This is me Ahmet Korkmaz This is me
Publication Date	February 7, 2011
Published in Issue	Year 2010 Volume: 44 Issue: 6

Cite

APA	Koca, K., Yurttas, Y., Yildiz, C., Cayci, T., et al. (2011). Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopaedica Et Traumatologica Turcica, 44(6), 476-483.
AMA	Koca K, Yurttas Y, Yildiz C, Cayci T, Uysal B, Korkmaz A. Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopaedica et Traumatologica Turcica. February 2011;44(6):476-483.
Chicago	Koca, Kenan, Yuksel Yurttas, Cemil Yildiz, Tuncer Cayci, Bulent Uysal, and Ahmet Korkmaz. “Effect of Hyperbaric Oxygen and Ozone Preconditioning on oxidative/nitrosative Stress Induced by Tourniquet ischemia/Reperfusion in Rat Skeletal Muscle”. Acta Orthopaedica Et Traumatologica Turcica 44, no. 6 (February 2011): 476-83.
EndNote	Koca K, Yurttas Y, Yildiz C, Cayci T, Uysal B, Korkmaz A (February 1, 2011) Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopaedica et Traumatologica Turcica 44 6 476–483.
IEEE	K. Koca, Y. Yurttas, C. Yildiz, T. Cayci, B. Uysal, and A. Korkmaz, “Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle”, Acta Orthopaedica et Traumatologica Turcica, vol. 44, no. 6, pp. 476–483, 2011.
ISNAD	Koca, Kenan et al. “Effect of Hyperbaric Oxygen and Ozone Preconditioning on oxidative/nitrosative Stress Induced by Tourniquet ischemia/Reperfusion in Rat Skeletal Muscle”. Acta Orthopaedica et Traumatologica Turcica 44/6 (February 2011), 476-483.
JAMA	Koca K, Yurttas Y, Yildiz C, Cayci T, Uysal B, Korkmaz A. Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopaedica et Traumatologica Turcica. 2011;44:476–483.
MLA	Koca, Kenan et al. “Effect of Hyperbaric Oxygen and Ozone Preconditioning on oxidative/nitrosative Stress Induced by Tourniquet ischemia/Reperfusion in Rat Skeletal Muscle”. Acta Orthopaedica Et Traumatologica Turcica, vol. 44, no. 6, 2011, pp. 476-83.
Vancouver	Koca K, Yurttas Y, Yildiz C, Cayci T, Uysal B, Korkmaz A. Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopaedica et Traumatologica Turcica. 2011;44(6):476-83.