Eremurus spectabilis Bieb.’in antioksidan ve antimikrobiyal özelliklerinin belirlenmesi

Abstract

Bu çalışmada, halk arasında sebze olarak tüketilen E. spectabilis Bieb.’in in vitro antioksidan ve antimikrobiyal aktivitesi incelenmiş ve anaerobik kültür ortamında Kluyveromyces lactis üzerinde ki antioksidan aktivitesi araştırılmıştır. Bu amaçla; 2,2-difenil-1-pikrilhidrazil (DPPH) giderme aktivitesi, flavonoid, resveratrol, şeker içeriği, lipid peroksidasyon düzeyi, yağ asidi düzeyi, lipofilik vitamin değerleri, protein ve glutatyon miktarları ölçüldü. FeCl2+H2O2 uygulanan FeCl grubunda LPO seviyesinin kontrol grubuna (K) kıyasla yüksek miktarda arttığı görüldü (p<0.05). Tedavi gruplarında da LPO düzeyinin belli oranlarda azaldığı belirlendi. Eremurus spectabilis’in LPO düşürücü etkisinin içerdiği flavonoidler gibi fitokimyasal kaynaklar olduğu ileri sürülmektedir. Antibakteriyel aktivite sonuçlarına göre E. spectabilis’in E. coli, S. aureus, Epidermophyton spp. ve C. albicans’a karşı farklı oranlarda antimikrobiyal aktiviteye sahip olduğu görülmektedir.

Keywords

• Antimikrobiyal aktivite,DPPH,E. spectabilis,lipid peroksidasyonu,maya kültürü,vitamin

Determination of antioxidant and antimicrobial properties of Eremurus spectabilis Bieb.

Abstract

In this study, in vitro antioxidant and antimicrobial activities of the E. spectabilis Bieb., which is consumed as a vegetable among people, was investigated and Kluyveromyces lactis’s antioxidant activies in anaerobic cultural environment is researched. This purpose; 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, flavonoid, resveratrol, sugar contents, lipid peroxidation (LPO) levels, fatty acid level, lipophilic vitamin values, protein and glutathione amounts were measured. In the FeCl group in which FeCl2+ H2O2 is applied, when compared with LPO level control group (K), it was observed that it increase in high amount (p<0.05). LPO level decreases in certain proportions in treatment groups. It is suggested that the lowering effect of the LPO of E. spectabilis extracts are arise from phytochemical like flavonoids that it includes. As a result of the antibacterial activity studies, it was observed that E. spectabilis have different proportional antimicrobial activities against E. coli, S. aureus, Epidermophyton spp. and C. albicans.

Keywords

• Antimicrobial activity,DPPH;,E. spectabilis,lipid peroxidation,vitamin,yeast culture

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