Dactylorhiza urvileana’nın in vitro asimbiyotik çimlendirilmesi ve fidelerinin oluşturulması

Abstract

Bu çalışmada Orchidaceae familyasına ait Dactylorhiza urvillenana (Steud.) H.Baumann & Künkele’nin doku kültürü yöntemleriyle çoğaltılması amaçlandı. Başlangıç eksplantı olarak türe ait olgunlaşmış tohumlar kullanıldı. Sterilizasyonları ve canlılık testleri yapılan tohumların çeşitli temel besi ortamlarındaki çimlenme, protokorm ve kallus oluşum oranları tespit edildi. Çimlenen tohumlardan oluşan protokormlar, Zeatin’in farklı derişimleri ile desteklenen Orchimax aktif kömürlü besi ortamında (OAM) organogeneze teşvik edildi ve sürgün uzama miktarları ve ortalama kök sayıları belirlendi. Elde edilen sonuçlara göre, çimlenme, protokorm ve kallus oluşumunda denenen temel besi ortamlarından Orchimax aktif kömürlü besi ortamının daha etkili olduğu görüldü. Bu ortamdaki çimlenme oranı  %62,39, protokorm oluşum oranı %73,18 ve kallus oluşum oranı %8,6 olarak belirlendi. Farklı zeatin derişimlerinin sürgün uzaması ve kök sayısı üzerine etkilerine bakıldığında, 1,0 mg/L derişiminin diğerlerinden daha olumlu sonuçlar verdiği görüldü ve bu ortamdan elde edilen ortalama sürgün boyu ve kök sayısı değerleri sırasıyla 36,05 mm ve 3,4 adet olarak hesaplandı.

Keywords

• Dactylorhiza urvileana,Doku kültürü,İn vitro,Protokorm,Asimbiyotik çimlenme

In vitro asymbiotic germination and plantlet formation of Dactylorhiza urvilleana

Abstract

In this study, in vitro production of Dactylorhiza urvilleana (Steud.) H.Baumann & Künkele by using plant cell and tissue culture techniques were carried out. Mature seeds were used as starting explant. After the surface sterilization and viability tests, seeds were germinated in different basal culture media then germination, protocorm and callus formation rates were determined. Protocorms were encouraged to organogenesis in Orchimax medium including activated charcoal (OAM) and supported with different concentrations of Zeatin and shoot length and root numbers of seedlings were detected. It has been determined that the most effective culture medium for germination, protocorm and callus formation rates of seeds is the OAM. In this medium, germination, protocorm and callus formation rates were determined as 62.39%, 73.18%, 8.6%, respectively. It has been determined that the media including 1.0 mg/L Zeatin were effective on the elongation of shoots and root formation. Shoot length and average root number was measured as 36.05 mm and 3.4, respectively.

Keywords

• Dactylorhiza urvileana,Tissue culture,In vitro,Protocorm,Asymbiotic germination

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