Prunus cerasifera cv. ‘’Pissardii Nigra’’nın antioksidan ve antimikrobiyal aktivitesi

Abstract

Bu çalışmada; Elazığ ilinde toplanan P. cerasifera cv. “Pissardi Nigra“ ekstraktlarının antioksidan ve antimikrobiyal aktiviteleri araştırıldı. Bitki özlerinin DPPH serbest radikal temizleme aktivitesi, lipid peroksidasyon önleme etkinliği ve vitamin içerikleri ölçüldü. Eksraktların 25 µl konsantrasyondan itibaren serbest radikal temizleme etkisi gösterdiği ve bu etkinin doza bağımlı arttığı tespit edildi. In vitro ortamda FeCI₂ grubunda lipid peroksidasyon düzeylerinin kontrol grubuna göre yüksek miktarlarda arttığı, meyve ekstraktı ve FeCI₂ içeren gruplardaki lipid peroksidasyon seviyelerinin belirli oranlarda azaldığı görüldü. Ekstraktların en fazla E vitamini (50.60-57.32 mg/g) ihtiva ettiği belirlendi. Ayrıca, meyve kısımlarından elde edilen özütlerin 13 bakteri (14-22 mm), 3 maya (14-18 mm) ve 2 dermofit fungus (16-18 mm) üzerine farklı düzeylerde antimikrobiyal etki gösterdikleri tespit edilmiştir.

Keywords

• Prunus,antimikrobial aktivite,DPPH,vitamin,LPO.

Antimicrobial and antioxidant activity of Prunus cerasifera cv. "Pissardii Nigra"

Abstract

In this study, antioksidant and antimicrobial activities of Prunus cerasifera cv. Pissardi Nigra collected from Elazığ were researched. The free radical scavenging activity (DPPH), lipid peroxidation inhibitory activity and vitamin amounts of plant extracts were measured. It showed that the free radical scavenging effect from 25 μl concentration of extracts. The effect was found to dose dependently increased. In vitro medium, it was determined that in FeCI2 group, lipid peroxidation amounts increases in a large ration with respect to the control group and lipid peroxidation levels in groups which includes plant extracts and FeCI2, decreases in certain amounts. According to results of the experiment, it was determined to contain a high amount of vitamin E in the extract (50.60-57.32 g/g). In addition, the antimicrobial activity studies of extract were shown to inhibit to different degrees the growth on thirteen bacteria (14-22 mm), three yeast (14-18 mm) and two dermatophyte (16-18 mm).

Keywords

• Prunus,antioxidant effect,antimicrobial activity,DPPH,lipid peroxidation

References

1. Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a freeradical method to evaluate antioxidant activity. Food Sci Technol-Leb 28: 25-30 .
2. Coccia A, Carraturo A, Mosca L, Masci A, Bellini A, Campagnaro M, Lendaro E ( 2012) Effects of methanolic extract of sour cherry (Prunus cerasus L.) on microbial growth. Inter J Food Sci Tech 47: 1620-1629.
3. Collins CH, Lyne PM (2004) Microbiological methods, eighth edition pp. 140, Butter Morths & Co (Publishers) Ltd., London.
4. Dowling C (2014) The polyphenolic composition and antioxidant capacity of yellow european plums (Prunus domestica L.) and novel golden prunes by carolyn dowling, BSC a thesis presented to the University of Guelph ın partial fulfillment of the requirements for the degree of Master of Science (MSc.) in Plant Agriculture University of Guelph Guelph, Ontario.
5. Gao HS, Liu SW, Zhao CM (2010) Compositional and nutritional studies on tomentosa cherry (Prunus tomentosa Thunb.) Fruit, 2nd Conference on Key Technology of Horticulture, 100-103.
6. Gündüz K, Saraçoğlu O (2012) Variation in total phenolic content and antioxidant activity of Prunus cerasifera Ehrh. selections from Mediterranean region of Turkey. Scientia Hortic 134: 88–92.
7. Jabeen Q, Aslam N (2011) The pharmacological activities of prunes: The dried Plums. J Med Plant Res 5: 1508-1511.Jabeen Q, Aslam N (2012) The pharmacological activities of prunes: The dried plums AJCS 6: 681-687.
8. Jimenez M, Castillo I, Azuara E, Beristain CI (2011) Antioxidant and antimicrobıal activty of capulın (Prunus serotina subsp. capuli) extracts. Revista Mexicana de Ingeniería Química 10: 29-37.

9. Kan T, Bostan Z (2010) Changes of contents of polyphenols and vitamin a of organic and conventional fresh and dried apricot cultivars (Prunus armeniaca L.). World J Agric Sci 6: 120-126.
10. Lopez-Cervantes J, Sanchez-Machado DI, Rios-Vazquez NJ (2006) High-performance liquid chromatography method for the simultaneous quantification of retinol, alpha-tocopherol, and cholesterol in shrimp waste hydrolysate. J Chromatogr A 1105: 135-139.
11. Mulabagal V, Lang GA, Dewitt DL, Dalavoy SS, Nair MG (2009) Anthocyanin content, lipid peroxidation and cyclooxygenase enzyme ınhıbıtory activities of sweet and sour cherries. J Agric Food Chem 57: 1239-1246.
12. Najafabad AM, Jamei R (2014) Free radical scavenging capacity and antioxidant activity of methanolic and ethanolic extracts of plum (Prunus domestica L.) in both fresh and dried samples. Avicenna J Phytomed 4: 343-353.
13. Ördögh L, Galgoczy L, Krisch J, Papp T, Vagvölgyi C (2010) Antioxidant and antimicrobial activities of fruit juices and pomace extracts against acne-inducing bacteria. Acta Biologica Szegediensis 54: 45-49.
14. Özsahin AD, Yılmaz O (2010) Fruit sugar, flavanoid and phytosterol contents of apricot fruits (Prunus armeniaca L. Kabaası) and antioxidant effects in the free radicals environment. Asian J Chem 22: 6403-6412.
15. Ronald BP (2001) Spectrophotometric measurement of secondary lipid oxidation products. Curr Protoc Food Analyt Chem D2.4.1–D2.4.8.
16. Senevıratne CJ, Wong R, Ha U, Chen Y, Herath T, Samaranayake P, Kao R (2011) Prunus mume extract exhibits antimicrobial activity against pathogenic oral bacteria. Int J Paediatr Dent 21: 299-300.
17. Voca S, Galic A, Šındrak Z, Dobricevic N, Pliestic SJ (2009) Chemical composition and antioxidant capacity of three plum cultivars. Agriculturae Conspectus Scientificus 74: 273-276
18. Walkowiak-Tomczak D, Reguła J, Łysiak G (2008) Physico-chemical properties and antioxidant activity of selected plum cultivars fruit. Łysiak Acta Sci Pol Technol Aliment 7: 15-22.
19. Wang Y, Chen Y, Zhang Y, Chen X (2012) Antioxidant activities and major anthocyanins of myrobalan plum (Prunus cerasifera Ehrh.). J Food Sci 77: 388-393.
20. Xia D, Shi J, Gong J, Wu X, Yang Q and Zhang Y (2010) Antioxidant activity of chinese mei (Prunus mume) and its active phytochemicals. J Med Plant Res 4: 1156-1160.
21. Yurdugül S, Bozoglu F (2009) Studies on antimicrobial activity and certain chemical parameters of freeze-dried wild plums (Prunus Spp.). Pak J Nutr 8: 1434-1441.