Against Drought Stress Effect of Antioxidant Enzymes of Boron

Abstract

In this study, soybean seeds (Glycine max. L., cv., “A3935) were grown under controlled conditions (25±2 C) composed of different boron compounds. In the experiment, 5 groups were determined respectively as potassium tetraborate tetrahydrate (1 mg/1), ammonium tetraborate tetrahydrate (1 mg/1), sodium boron hydride (1 mg/1), lithium tetraborate tetrahydrate (100 mg/1), and sodium tetraborate decahydrate (100 mg/1). The doses used in this study were determined according to the results of a preliminary study. Soybean seeds were exposed to different amounts of drought stress based on time (control, 3, 6, 9, 12, 15, and 18 days). Activities of antioxidant enzymes superoxide dismutase (SOD: EC 1.15.1.1), glutathione reductase (GR: EC 1.6.4.2), ascorbate peroxidase (APX: EC 1.11.1.11) and catalase (CAT: EC 1.11.1.6) measured. According to the results stress+potassium tetraborate tetrahydrate environment has increased the amount of CAT, decreased the amount GR, APX and SOD. Potassium tetraborate 0.1 mg / l dose administration is the most appropriate critical value, and the most important indicator of drought CAT enzyme found to give the best results.

Keywords

• Drought stress
• soybean
• boron
• antioxidative enzymes

Kuraklık Stresine Karşı Borun Antioksidant Enzimlere Etkisi

Abstract

Bu çalışmada materyal olarak soya (Glycine max. L., cv., “A3935”) tohumları farklı bor bileşiklerinden oluşan, kontrollü koşullarda (25+2 C) yetiştirilmiştir. Araştırmada kontrol grubuna herhangi bir bor uygulaması yapılmamıştır. Diğer uygulamalar sırasıyla; potasyum tetraborat tetrahidrattan 0.1 mg/l, amonyum tetraborat tetrahidrattan 1 mg/l, sodyum bor hidrürden 1 mg/l, lityum tetraborat tetrahidrattan 100 mg/l, sodyum tetraborat dekahidrattan 100 mg/l olmak üzere belirlenen uygun doz kullanılmıştır. Araştırma tesadüf parselleri deneme desenine göre planlanmıştır. Soya fideleri zamana bağlı olarak farklı kuraklık (kontrol, 3, 6, 9, 12, 15 ve 18 gün) uygulamalarına maruz bırakılmıştır. Yapraklarda katalaz (CAT; EC= 1.11.1.6), gulutatyon redüktaz (GR; EC= 1.6.4.2), askorbat peroksidaz (APX; EC= 1.11.1.11) ve süperoksit dismutaz (SOD; EC= 1.15.1.1) enzim aktiviteleri ölçülmüştür. Analiz sonuçlarına göre Stres+potasyum tetraborat tetrahidrat ortamında CAT miktarı artmış, GR, APX ve SOD miktarı azalmıştır. Potasyum tetraboratın 0.1 mg/l doz uygulamasının en uygun eşik değeri olduğu ve kuraklığa karşı en önemli gösterge olan CAT enziminde en iyi sonucu verdiği anlaşılmıştır.

Keywords

• kuraklık stresi
• soya
• bor
• antioksidant enzimler

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