Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources

Abstract

Genetic diversity in natural stands of autochthonous blackthorn (Prunus spinosa L.) of different German provenances has been analyzed using a highly reproducible high-annealing-temperature random amplified polymorphic DNA (HAT-RAPD) protocol. The findings were compared to those from seedstocks of the same provenances, reported earlier. Generally, genetic diversity in the natural stands was even lower (H_o 0.099–0.116) compared to the corresponding seedstocks (H_o 0.118–0.133). Furthermore, genetic differentiation was found to be moderate between natural residential sources (pairwise F_st 0.138–0.184, 22.527% variation among populations), but higher than between the seedstocks (pairwise F_st 0.086-0.104, 7.782% variation among populations). The findings are discussed in respect to German conservation law and its practical implementation.  

Keywords

• Prunus spinosa; autochthonous; RAPD; genetic diversity; differentiation

Prunus Spinosa L.’nin Genetik Çeşitliliği ve Yerli Popülasyon Kaynaklarının Kullanımındaki Zorluklar

Abstract

Almanya’nın farklı bölgelerindeki çakal eriği (Prunus spinosa L.) doğal türünün genetik çeşitliliği, yüksek yapışma sıcaklığında rastgele çoğaltılmış polimorfik DNA (HAT-RAPD) protokolü kullanılarak analiz edilmiştir. Bulgular daha önceki çalışmalarda kaydedilen benzer bölgelerden toplanmış tohum stoklarından elde edilen örneklerin bulgularıyla karşılaştırılmıştır. Genellikle doğal türlerdeki (suş) genetik çeşitlilik önceki çalışmalarda ölçülen değerden (Ho: 0,118-0,113) düşük olduğu (Ho; 0,099-0,116) belirlenmiştir. Dahası genetik farklılaşma doğal türlerde orta düzeyde (Fst; 0,138- 0,184; populasyonlar arası varyasyon oranı % 22,527) ama tohum stoklarından elde edilen örneklerin değerlerinden (Fst; 0,0860,104; populasyonlar arası varyasyon oranı % 7,782) daha yüksek olduğu belirlenmiştir. Bulgular Almanya koruma kanunları ve uygulamaları bakımından tartışılmıştır

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