Biological activity potential of Thalictrum orientale: antioxidant, anticholinesterase, and antiproliferative effects

Year 2025, Volume: 26 Issue: 1, 222 - 229, 15.05.2025

Oğuzhan Koçer

https://doi.org/10.17474/artvinofd.1667174

Cited By: 1

Abstract

Plants are among the important natural resources in terms of biological activity. In this study, the biological activity of Thalictrum orientale species was evaluated comprehensively. For this purpose, ethanol extract of the plant was obtained by Soxhlet extraction method. To determine the antioxidant capacity, total antioxidant status (TAS) and total oxidant status (TOS) were analyzed using Rel Assay kits. Anticholinesterase activity was determined by measuring acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme activities. In addition, antiproliferative activity was evaluated on A549 lung cancer cell line. As a result of the analyzes, TAS value of Thalictrum orientale was determined as 5.413±0.149 mmol/L, TOS value as 8.300±0.056 µmol/L and oxidative stress index (OSI) as 0.154±0.004. Within the scope of anticholinesterase activity, AChE inhibition value was determined as 35.41±1.88 µg/mL and BChE inhibition value was determined as 58.63±1.24 µg/mL. It was observed that the antiproliferative effect increased in direct proportion to the increase in concentration. The findings show that Thalictrum orientale has high biological potential in terms of antioxidant, antiproliferative and anticholinesterase activities.

Keywords

Thalictrum orientale , antioxidant activity , anticholinesterase activity , antiproliferative activity , biological activity

Thalictrum orientale'nin biyolojik aktivite potansiyeli: antioksidan, antikolinerjik ve antiproliferatif etkiler

Abstract

Bitkiler, biyolojik aktivite açısından önemli doğal kaynaklar arasında yer almaktadır. Bu çalışmada, Thalictrum orientale türünün biyolojik aktivitesi kapsamlı bir şekilde değerlendirilmiştir. Bu amaçla, bitkinin etanol özütü Soxhlet ekstraksiyon yöntemiyle elde edilmiştir. Antioksidan kapasitesinin belirlenmesi için toplam antioksidan statusu (TAS) ve toplam oksidan statusu (TOS) Rel Assay kitleri kullanılarak analiz edilmiştir. Antikolinesteraz aktivitesi, asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) enzim aktiviteleri ölçülerek belirlenmiştir. Ayrıca, antiproliferatif aktivite, A549 akciğer kanser hücre hattı üzerinde değerlendirilmiştir. Yapılan analizler sonucunda Thalictrum orientale’in TAS değeri 5.413±0.149 mmol/L, TOS değeri 8.300±0.056 µmol/L ve oksidatif stres indeksi (OSI) 0.154±0.004 olarak belirlenmiştir. Antikolinesteraz aktivitesi kapsamında AChE inhibisyon değeri 35.41±1.88 µg/mL, BChE inhibisyon değeri ise 58.63±1.24 µg/mL olarak saptanmıştır. Antiproliferatif etkinin, konsantrasyon artışı ile doğru orantılı olarak arttığı gözlemlenmiştir. Elde edilen bulgular, Thalictrum orientale’in antioksidan, antiproliferatif ve antikolinesteraz aktiviteleri açısından yüksek biyolojik potansiyele sahip olduğunu göstermektedir.

Keywords

Thalictrum orientale , antioksidan aktivite , antiproliferatif aktivite , biyolojik aktivite , Antikolinesteraz aktivite

References

• Akgul H, Korkmaz N, Dayangaç A, Sevindik M (2020) Antioxidant potential of endemic Salvia absconditiflora. Turkish Journal of Agriculture-Food Science and Technology, 8(10): 2222-2224.
• Alzahrani AR, Hosny N, Mohamed DI, Nahas HHA, Albogami A, Al-Hazani TMI, Saied EM (2024) Unveiling the multifaceted antiproliferative efficacy of Cichorium endivia root extract by dual modulation of apoptotic and inflammatory genes, inducing cell cycle arrest, and targeting COX-2. RSC Advances, 14(27): 19400-19427.
• Bal C, Akgul H, Sevindik M, Akata I, Yumrutas O (2017) Determination of the anti-oxidative activities of six mushrooms. Fresenius Envir Bull, 26(10): 6246-6252.
• Chen ZJ, Yang HG, Zhu WP, Xue CM, Zhang HM, Peng YT, Hua HM (2024) New aporphine alkaloids with antitumor activities from the roots of Thalictrum omeiense. Journal of Asian Natural Products Research, 1-9.
• Cichon N, Grabowska W, Gorniak L, Stela M, Harmata P, Ceremuga M, Bijak M (2024) Mechanistic and therapeutic ınsights into flavonoid-based ınhibition of acetylcholinesterase: ımplications for neurodegenerative diseases. Nutrients, 17(1): 78.
• El-Chaghaby GA, Mohammed FS, Rashad S, Uysal I, Koçer O, Lekesiz Ö, Sevindik M (2024) Genus hypericum: general properties, chemical contents and biological activities. Egyptian Journal of Botany, 64(1): 1-26.
• Ellman GL, Courtney KD, Andres JrV, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2): 88-95.
• Erdemgil FZ, Telezhenetskaya MV, Levkovich MG, Abdullaev ND, Kirimer N, Baser KHC (2001) Alkaloids of Thalictrum orientale. Chemistry of Natural Compounds, 37: 295-296.
• Erdemgil FZ, Baser KHC, Akbay P, Sticher O, Çalis I (2003) Thalictricoside, a new phenolic compound from Thalictrum orientale. Zeitschrift für Naturforschung, C, 58(9-10): 632-636.
• Erel O (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4): 277-285.
• Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12): 1103-1111.
• Gu C, Howell K, Dunshea FR, Suleria HA (2019) LC-ESI-QTOF/MS characterisation of phenolic acids and flavonoids in polyphenol-rich fruits and vegetables and their potential antioxidant activities. Antioxidants, 8(9): 405.
• Gürgen A, Sevindik M, Yıldız S, Akgül H (2020) Determination of antioxidant and oxidant potentials of Pleurotus citrinopileatus mushroom cultivated on various substrates. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(3): 586-591.
• Gürgen A, Sevindik M (2022) Application of artificial neural network coupling multiobjective particle swarm optimization algorithm to optimize Pleurotus ostreatus extraction parameters. Journal of Food Processing and Preservation, 46(11): e16949.
• Gürgen A, Sevindik M, Krupodorova T, Uysal I, Unal O (2024) Biological activities of Hypericum spectabile extract optimized using artificial neural network combined with genetic algorithm application. BMC biotechnology, 24(1): 83.
• Jamal QMS, Khan MI, Alharbi AH, Ahmad V, Yadav BS (2023) Identification of natural compounds of the apple as inhibitors against cholinesterase for the treatment of Alzheimer’s disease: an in silico molecular docking simulation and ADMET study. Nutrients, 15(7): 1579.
• Jiang SQ, Zhang YB, Xiao M, Jiang L, Luo D, Niu QW, Wang GC (2017) Cycloartane triterpenoid saponins from the herbs of Thalictrum fortunei. Carbohydrate Research, 445: 1-6.
• Meng F, Wei X, Sun Y, Zeng Q, Wang G, Lan X, Chen M (2021) Cytotoxic triterpenoid saponins from Thalictrum atriplex. Natural Product Research, 35(24): 5757-5764.
• Mishra N, Jiang C, Chen L, Paul A, Chatterjee A, Shen G (2023) Achieving abiotic stress tolerance in plants through antioxidative defense mechanisms. Frontiers in Plant Science, 14: 1110622.
• Mohammed FS, Korkmaz N, Doğan M, Şabik, AE, Sevindik M (2021) Some medicinal properties of Glycyrrhiza glabra (Licorice). Journal of Faculty of Pharmacy of Ankara University, 45(3): 524-534.
• Mohammed FS, Sevindik M, Uysal I, Sevindik E, Akgül H (2022) A natural material for suppressing the effects of oxidative stress: biological activities of Alcea kurdica. Biology Bulletin, 49(Suppl 2): S59-S66.
• Mohammed FS, Uysal I, Sevindik M (2023) A review on antiviral pants effective against different virus types. Prospects in Pharmaceutical Sciences, 21(2): 1-21.
• Mohammed FS, Sevindik M, Uysal İ, Çesko C, Koraqi H (2024) Chemical composition, biological activities, uses, nutritional and mineral contents of cumin (Cuminum cyminum). Measurement: Food, 100157.
• Nik Salleh NNH, Othman FA, Kamarudin NA, Tan SC (2020) The biological activities and therapeutic potentials of baicalein extracted from Oroxylum indicum: a systematic review. Molecules, 25(23): 5677.
• Oran SA, Yousef I, Jaffal SM (2022) Evaluation of the antiproliferative effect of selected plant extracts on colon and skin cancer cell lines. Journal of Applied Pharmaceutical Science, 12(11): 091-096.
• Sabik AE, Sevindik M, Mohammed FS, Dogan M (2024) A new natural source against A549 lung cancer cells anthemis cotula and its biological activities and phenolic contents. Pharmaceutical Chemistry Journal, 58(7).
• Savchenko T, Degtyaryov E, Radzyukevich Y, Buryak V (2022) Therapeutic potential of plant oxylipins. International Journal of Molecular Sciences, 23(23): 14627.
• Sevindik M (2019) The novel biological tests on various extracts of Cerioporus varius. Fresenius Environmental Bulletin, 28(5): 3713-3717.
• Sevindik M, Akgul H, Dogan M, Akata I, Selamoglu Z (2018) Determination of antioxidant, antimicrobial, DNA protective activity and heavy metals content of Laetiporus sulphureus. Fresenius environmental Bulletin, 27(3):1946-1952.
• Sevindik M, Akgul H, Selamoglu Z, Braidy N (2020) Antioxidant and antigenotoxic potential of Infundibulicybe geotropa mushroom collected from Northwestern Turkey. Oxidative Medicine and Cellular Longevity, 2020(1): 5620484.
• Sevindik M, Mohammed FS, Uysal I (2023) Autism: plants with neuro-psychopharmacotherapeutic potential. Prospects in Pharmaceutical Sciences, 21(3): 38-48.
• Sevindik M, Gürgen A, Krupodorova T, Uysal İ, Koçer O (2024) A hybrid artificial neural network and multi-objective genetic algorithm approach to optimize extraction conditions of Mentha longifolia and biological activities. Scientific Reports, 14(1): 31403.
• Sevindik M, Koçer O, Korkmaz N, Yüzbaşıoğlu MA, Uysal İ (2025) Some biological activities of Thalictrum minus (Ranunculaceae). International Journal of Secondary Metabolite, 12(1): 46-53.
• Singh H, Singh D, Lekhak MM (2023) Ethnobotany, botany, phytochemistry and ethnopharmacology of the genus Thalictrum L. (Ranunculaceae): a review. Journal of Ethnopharmacology, 305: 115950.
• Turkmen D, Dursun A, Caliskan O, Koksal Kavrak M, Guler Z (2023) Volatile compounds, phenolic content, and antioxidant capacity in Sultan hawthorn (Crataegus azarolus L.) leaves. Journal of Agricultural Science and Technology, 25(5): 1089-1099.
• Uysal I, Sevindik M, Dogan M, Mohammed FS, Seğmenoğlu MS, Pehlivan M (2024) Biological activities, phenolic contents and chemical compositions of essential oils and different extracts of Origanum laevigatum. Pharmaceutical Chemistry Journal, 58(8): 1289-1297.
• Yazar M, Sevindik M, Polat AO, Koçer O, Karatepe HK, Uysal İ (2024) General properties, biosynthesis, pharmacological properties, biological activities and daily uses of luteolin. Prospects in Pharmaceutical Sciences, 22(4): 146-154.