Determination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya-Terziköy Thermal Spring

Abstract

Actinobacteria represent a group of Gram-positive microorganisms characterized by high G+C content and are commonly found in both terrestrial and aquatic ecosystems. These bacteria are notable for their capacity to synthesize a wide range of bioactive compounds, including antibiotics and industrial enzymes. Their ability to withstand extreme environmental conditions allows them to colonize challenging habitats, such as thermal springs. These geothermal environments are critical not only for harboring microbial diversity but also for the discovery of novel bioactive substances with therapeutic potential. This study focused on isolating thermophilic actinobacteria from Terziköy Thermal Spring in Amasya, Türkiye, followed by phylogenetic analysis through 16S rRNA gene sequencing. The enzymatic capacities of the isolates—specifically amylase and protease production—were also assessed. Fourteen of the 21 isolates underwent 16S rRNA sequencing, resulting in the identification of eight Streptomyces, four Nocardia, one Actinomadura, and one Pseudonocardia species. Among these, seven isolates demonstrated amylase activity, and one displayed protease production. Notably, one Streptomyces isolate exhibited both enzymatic activities. These results highlight the phylogenetic diversity of thermophilic actinobacteria in geothermal environments and their potential as sources of thermostable enzymes for industrial applications.

Keywords

• Thermophilic bacteria
• 16S rRNA gene
• amylase and protease activities

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