Pseudomonas fluorescens BALIK PATOJENİNDE ÇEVREYİ ALGILAMA SİSTEMİ VE VİRÜLENS FAKTÖRLERİ

Abstract

Araştırmada Gram negatif bakteriyel balık patojenlerinden Pseudomonas fluorescens’in üç adet suşu kullanılmıştır. Suşlar da öncelikle N-butanoyl-L-homoserin lakton (BHL) ve N-(3-okzododekanoyl)-L-homoserin lakton (OdDHL) sinyal moleküllerinin üretimi, Chromobacterium violaceum CV026 ve Agrobacterium tumafeciens NT1 biyosensör suşları kullanılarak test edilmiştir. Pseudomonas aeruginosa PAO1 suşu pozitif kontrol olarak kullanılmıştır. Sonuç olarak, P. fluorescens suşları, C. violaceum CV026 suşu kullanıldığında BHL sinyal molekülünü üretmediği, bununla birlikte, A. tumafeciens NT1 kullanıldığında, P. fluorescens suşlarının OdDHL sinyal molekülünü ürettiği tespit edilmiştir. P. fluorescens suşları; biyofilm, pigment, ramnolipid, elastaz, amilaz, hemoliz ve proteaz aktiviteleri gibi virülens faktörlerini üretmiştir. 

Keywords

• P. fluorescens,Çevreyi algılama sistemi,Virülens faktörleri,Biyofilm,Pigment,Ramnolipid

QUORUM SENSING SYSTEM AND VIRULENCE FACTORS IN Pseudomonas fluorescens FISH PATHOGEN

Abstract

In this study three Pseudomonas fluorescens strains, Gram-negative fish pathogen were used.  Previously N-butanoyl-L-homoserine lacton (BHL) and N-(3 okzododekanoyl)-L-homoserine lacton (OdDHL) signal molecules of production of strains were examined using biosensor strains, Choromobacterium violaceum CV026 strain and Agrobacterium tumefaciens NT1. Pseudomonas aeruginosa PAO1 strain used to as positive control. As a results of this study; P. fluorescens strains did not produce BHL signal molecules using C. violaceum CV026.  However, the strains produced OdDHL signal molecules using A. tumafeciens NT1. It was determined production of biofilm, pigment, rhamnolipid, elastase, protease, amilase and hemolysis activity as phenotype tested as virulence factors in the strains.  

Keywords

• P. fluorescens,Quorum sensing system,Virulence factors,Biofilm,Pigment,Rhamnolipid

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