Fungal and Bacterial Co-Infection of Sea Bass (Dicentrarchus labrax, Linnaeus 1758) in a Recirculating Aquaculture System: Saprolegnia parasitica and Aeromonas hydrophila

Year 2018, Volume: 33 Issue: 3, 67 - 71, 28.06.2018

Ezgi Dinçtürk , Tevfik Tansel Tanrıkul Saniye Türk Çulha

Abstract

DOI: 10.26650/ASE201811

Aeromonas
hydrophila causes symptoms of hemorrhagic septicemia in acute cases and can
cause death in freshwater fish, whereas Saprolegnia parasitica is the cause of
ulcers complicated by fungal mycelia located on skin that has lost its scales
for various reasons. Both pathogens co-infect recirculating aquaculture systems
at the İzmir Katip Çelebi University Fisheries Research and Training Center.
Clinical, bacteriological, parasitological, and mycological studies were
carried out on 25 fish samples during the infestation. Sabouraud glucose agar
and malt extract agar were used to isolate the fungus, and the bacterial
isolates were streaked on tryptic soy agar (Oxoid) with 5% defibrinated sheep
blood. Gray-white cotton-like patches, erosions on the skin, and hemorrhaging
were detected on the infected fish samples. The analytical profile index test
and molecular identification showed that the bacterial agent was A. hydrophila
and a fungal examination and amplification by polymerase chain reaction
confirmed that the mycotic agent was S. parasitica.

Keywords

Aeromonas hydrophila, Dicentrarchus labrax, Saprolegnia parasitica

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