Effect of Polypropylene Microplastic and Florfenicol Antibiotic on Some Hormonal and Haematological Biomarkers in Yellowfin Seabream (Acanthopagrus latus)

Year 2025, Volume: 40 Issue: 2, 116 - 123, 25.04.2025

Mehrnaz Shirmohammadi , Farahnaz Kianersi , Nima Shiry , Fatemeh Hekmatpour

https://doi.org/10.26650/ASE20251638853

Abstract

Microplastics (MPs) and antibiotics, such as florfenicol (FFC), are emerging pollutants affecting aquatic ecosystems. This research examined the separate and joint impacts of MPs and FFC on hematological and hormonal indicators in yellowfin seabream (Acanthopagrus latus). The fish were categorized into four groups according to their dietary treatments: a control group with a standard diet and three experimental groups receiving diets containing 15 mg/kg of FFC, 100 mg/kg of MPs, or both for 10-day. The ELISA method was used to measure hormones in plasma. Blood collection occurred on the first, fourth, seventh, and fourteenth days after feeding stopped to evaluate FFC concentration in the plasma and various hematological and hormonal parameters. Exposure to FFC and MPs, alone or together, considerably decreased red blood cell, hemoglobin levels, hematocrit values, mean corpuscular hemoglobin, mean corpuscular hemoglobin, white blood cell, lymphocyte, and erythropoietin concentrations. Neutrophil and cortisol levels increased. Mean corpuscular volume was elevated only in the group receiving both FFC and MPs. After a 14- day recovery period, all measured parameters returned to baseline levels in the FFC-only group. Co-exposed group showed the highest concentration of FFC in the plasma on the first day. The groups administered MPs, individually or collectively, exhibited a reduction in thyroid hormones. These findings indicate that both MPs and FFC induce anemia and stress in yellowfin seabream, with co-exposure exacerbating these effects. Although the toxic effects of FFC were temporary, the lasting presence of MPs indicates potential long-term risks.

Keywords

Yellowfin seabream , Acanthopagrus latus , Florfenicol , Haematological/ Hormonal indices , Microplastics

Ethical Statement

The experiments were conducted in accordance with the guidelines of the National Ethical Committee for Animal Research in Iran

Thanks

The authors would like to thank Khorramshahr University of Marine Science and Technology for providing laboratory space

References

• Ammar, E., Hamed, M., Mohamed, M.S., & Sayed, A.E.H. (2023). The synergetic effects of 4-nonylphenol and polyethylene microplastics in Cyprinus carpio juveniles using blood biomarkers. Scientific reports, 13(1), 11635. https://doi.org/10.1038/s41598-023-38636-2 google scholar
• Akbari, A., & Jaafari, J. (2025). The effects of microplastic pollution on marine organisms and its impact on human health: A review. Caspian Journal of Health Research, 10 (1), 21-36. https://doi.org/10.32598/ CJHR.10.1.1925.1 google scholar
• Athira, T.R., Aarif, K.M., Thomas, J.A., Alatawi, A.S., Muzaffar, S.B., Nefla, A. et al. (2024). The threat of microplastics: Exploring pollution in coastal ecosystems and migratory shorebirds along the west coast of India. Marine pollution bulletin, 198, 115912. https://doi.org/10.1016/j.marpolbul.2023.115912 google scholar
• Azam, S.M. (2025). Effect of Microplastic Ingestion on Digestive Enzymes, Hormones, Hematology and Serum Biochemistry of Gallus gallus domesticus. Egyptian Journal of Veterinary Sciences, 56(6), 13171326. https://doi.org/10.21608/EJVS.2024.276205.1905 google scholar
• Banaee, M., Faraji, J., Amini, M., Multisanti, C.R., & Faggio, C. (2023). Rainbow trout (Oncorhynchus mykiss) physiological response to microplastics and enrofloxacin: Novel pathways to investigate microplastic synergistic effects on pharmaceuticals. Aquatic Toxicology, 261, 106627. https://doi.org/ 10.1016/j.aquatox.2023.106627 google scholar
• Bardhan, A., Abraham, T.J., Das, R., & Patil, P.K. (2024). Unraveling florfenicol’s effects on splenic histology, erythrocytes, and hematology of healthy Oreochromis niloticus juveniles. Applied Research, 3(5), e202400017. https://doi.org/10.1002/appl.202400017 google scholar
• Bardhan, A., Abraham, T.J., Singha, J., Rajisha, R., Krishna, E.K.N., Panda, S.K. et al. (2023). Impacts of oral florfenicol medication and residues on the kidney and liver of Nile tilapia Oreochromis niloticus (L.). Veterinary Sciences, 10(1), 36. https://doi.org/10.3390/vetsci10010036] google scholar
• Bardhan, A., Abraham, T.J., Das, R., & Patil, P.K. (2022). Biological Responses of Nile tilapia Oreochromis niloticus as influenced by dietary florfenicol. Toxics, 10(10), 571. https://doi.org/10.3390/toxics10100571 google scholar
• Barry, T.P., Lapp, A.F., Kayes, T.B., & Malison, J.A. (1993). Validation of a microtitre plate ELISA for measuring cortisol in fish and comparison of stress responses of rainbow trout (Oncorhynchus mykiss) and lake trout (Salvelinus namaycush). Aquaculture, 117(3-4), 351-363. https:// doi.org/10.1016/0044-8486(93)90331-R google scholar
• Clauss, T.M., Dove, A.D., & Arnold, J.E. (2008). Hematologic disorders of fish. Veterinary clinics of North America: Exotic animal practice, 11(3), 445-462. https://doi.org/10.1016/j.cvex.2008.03.007 google scholar
• Datta, N., Kar, P.K., & Saha, S.K. (2022). Circulatory physiology and erythropoiesis in freshwater fish Labeo rohita experimentally parasitized by Argulus bengalensis. Journal of Applied Ichthyology, 38(1), 63-72. https://doi.org/10.1111/jai.14287 google scholar
• Del Piano, F., Lama, A., Monnolo, A., Pirozzi, C., Piccolo, G., Vozzo, S. et al. (2023). Subchronic exposure to polystyrene microplastic differently affects redox balance in the anterior and posterior intestine of sparus aurata. Animals, 13(4), 606. https://doi.org/ 10.3390/ani13040606 google scholar
• Ding, P., Han, Y., Sun, Y., Chen, X., Ge, Q., Huang, W. et al. (2025). Synergistic neurotoxicity of clothianidin and photoaged microplastics in zebrafish: Implications for neuroendocrine disruption. Environmental Pollution, 316, 125797. https://doi.org/10.1016/j. envpol.2025.125797 google scholar
• Harikrishnan, T., Paramasivam, P., Sankar, A., Sakthivel, M., Sanniyasi, E., Raman, T. et al. (2024). Weathered polyethylene microplastics induced immunomodulation in zebrafish. Environmental Toxicology and Pharmacology, 109, 104478. https://doi.org/ 10.1016/j. etap.2024.104478 google scholar
• Hoseini, S.M., & Yousefi, M. (2019). Beneficial effects of thyme (Thymus vulgaris) extract on oxytetracycline-induced stress response, immunosuppression, oxidative stress and enzymatic changes in rainbow trout (Oncorhynchus mykiss). Aquaculture nutrition, 25(2), 298-309. https://doi.org/10.1111/anu.12853 google scholar
• Hossain, M.B., Pingki, F.H., Azad, M.A.S., Nur, A.A.U., Banik, P., Paray, B.A. et al. (2023). Microplastics in Different Tissues of a Commonly Consumed Fish, Scomberomorus guttatus, from a Large Subtropical Estuary: Accumulation, Characterization, and Contamination Assessment. Biology, 12(11), 1422. https://doi.org/10.3390/biology12111422 google scholar
• Jangaran Nejad, A., Peyghan, R., Najafzadeh Varzi, H., & Shahriyari, A. (2017). Florfenicol pharmacokinetics following intravenous and oral administrations and its elimination after oral and bath administrations in common carp (Cyprinus carpio). Veterinary Research Forum, 8(4), 327-331. google scholar
• Jeyavani, J., Sibiya, A., Stalin, T., Vigneshkumar, G., Al-Ghanim, K.A., Riaz, M.N. et al. (2023). Biochemical, genotoxic and histological implications of polypropylene microplastics on freshwater fish Oreochromis mossambicus: An aquatic eco-toxicologicalassessment. Toxics, 11(3), 282. https://doi.org/10.3390/toxics11030282 google scholar
• Kondera, E., Bojarski, B., tugowska, K., Kot, B., & Witeska, M. (2020). Effects of oxytetracycline and gentamicin therapeutic doses on hematological, biochemical and hematopoietic parameters in Cyprinus carpio juveniles. Animals, 10(12), 2278. https://doi. org/10.3390/ani10122278 google scholar
• Kverme, K.O., Haugland, G.T., Hannisdal, R., Kallekleiv, M., Colquhoun, D.J., Lunestad, B.T. et al. (2019). Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration. Aquaculture, 512, 734279. https://doi.org/10.1016/j.aquaculture.2019.734279 google scholar
• Lai, J.C., Kakuta, I., Mok, H.O., Rummer, J.L., & Randall, D. (2006). Effects of moderate and substantial hypoxia on erythropoietin levels in rainbow trout kidney and spleen. Journal of Experimental Biology, 209(14), 2734-2738. https://doi.org/10.1242/jeb.02279 google scholar
• Lemos, L.S., Angarica, L.M., Hauser-Davis, R.A., & Quinete, N. (2023). Cortisol as a stress indicator in fish: sampling methods, analytical techniques, and organic pollutant exposure assessments. International Journal of Environmental Research and Public Health, 20(13), 6237. https://doi.org/10.3390/ijerph20136237 google scholar
• Li, W., Zou, H., Zheng, Y., Zhang, G., Xiang, Y., Zhi, D. et al. (2025). Microplastics in aquatic environments: detection, abundance, characteristics, and toxicological studies. Environmental Monitoring and Assessment, 197(2), 1-22. https://doi.org/10.1007/s10661-024-13605-y google scholar
• Maghsodian, Z., Sanati, A.M., Ramavandi, B., Ghasemi, A., & Sorial, G.A. (2021). Microplastics accumulation in sediments and Periophthalmus waltoni fish, mangrove forests in southern Iran. Chemosphere, 264, 128543. https://doi.org/ 10.1016/j.chemosphere.2020.128543 google scholar
• Nair, H.T., & Perumal, S. (2024). Growth performance, hematological and oxidative stress responses in Nile tilapia (Oreochromis niloticus) exposed to polypropylene microplastics. Environmental Quality Management, 34(1), e22179. https://doi.org/10.1002/tqem.22179 google scholar
• Nwani, C.D., Mkpadobi, B.N., Onyishi, G., Echi, P.C., Chukwuka, C.O., Oluah, S.N. et al. (2014). Changes in behavior and hematological parameters of freshwater African catfish Clarias gariepinus (Burchell 1822) following sublethal exposure to chloramphenicol. Drug and chemical toxicology, 37(1), 107-113. https://doi.org/10.3109/014805 45.2013.834348 google scholar
• Patil, P.M., Mahamuni-Badiger, P., Ingavale, R.R., Patel, P.R., & Dhanavade, M.J. (2024). Usage of Microplastic Beads in Pharmaceuticals and Cosmetics Industry: A Review. Microplastic Pollution, 51-72. https:// doi.org/ 10.1007/978-981-99-8357-5_4 google scholar
• Sadeghi, P., Kazerouni, F., Savari, A., Movahedinia, A., Safahieh, A., & Ajdari, D. (2015). Evaluation of Changes in Erythropoietin Hormone Levels and Renal Pathological Lesion in Epaulet Grouper (Epinephelus stoliczkae) in Response to Chromium. Journal of Oceanography, 6(22), 115-127. google scholar
• Sankar, S., Chandrasekaran, N., Moovendhan, M., & Parvathi, V.D. (2025). Zebrafish and Drosophila as Model Systems for Studying the Impact of Microplastics andNanoplastics-A Systematic Review. Environmental Quality Management, 34(3), e70021. https://doi.org/10.1002/ tqem.70021 google scholar
• Shirmohammadi, M., Kianersi, F., Shiry, N., Burgos-Aceves, M.A., & Faggio, C. (2024). Biotransformation and oxidative stress markers in yellowfin seabream (Acanthopagrus latus): Interactive impacts of microplastics and florfenicol. Science of the Total Environment, 954, 176661. https://doi.org/10.1016/j.scitotenv.2024.176661 google scholar
• Shirmohammadi, M., Salamat, N., Ronagh, M.T., Movahedinia, A., & Hamidian, G. (2017). Assessment of immune status of yellowfin seabream (Acanthopagrus latus) during short term exposure to phenanthrene. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 195, 78-90. https://doi.org/10.1016/j. cbpc.2017.02.012 google scholar
• Shirmohammadi, M., Salamat, N., Ronagh, M.T., Movahedinia, A., & Hamidian, G. (2018). The Effect of Phenanthrene on Some Hematological Indices Yellowfin Seabream (Acanthopagrus latus). Iranian Journal of Toxicology, 12(1), 47-52. https://doi.org/10.29252/ arakmu.12.1.47 google scholar
• Shiry, N., Soltanian, S., Shomali, T., & Salighehzadeh, R. (2020). Effects of Oral Administration of Florfenicol on Some Hematological Indices of Rainbow Trout (Oncorhynchus mykiss) Challenged with Streptococcosis/Lactococcosis Agents. Journal of Veterinary Research, 75(3), 320-327. https://doi.org/10.22059/JVR.2019.273876.2890 google scholar
• Wang, J., Li, X., Li, P., Li, L., Zhao, L., Ru, S. et al. (2022). Porous microplastics enhance polychlorinated biphenyls-induced thyroid disruption in juvenile Japanese flounder (Paralichthys olivaceus). Marine Pollution Bulletin, 174, 113289. https://doi.org/10.1016/j.marpolbul.2021.113289 google scholar
• Yedier, S., Yalçınkaya, S.K., & Bostancı, D. (2023). Exposure to polypropylene microplastics via diet and water induces oxidative stress in Cyprinus carpio. Aquatic Toxicology, 259, 106540. https:// doi.org/10.1016/j.aquatox.2023.106540 google scholar
• Yi, C., Shang, J., Shen, Z., Sun, Y., Yang, Y., Zheng, X. et al. (2025). Distribution and risk characteristics of antibiotics in China surface water from 2013 to 2024. Chemosphere, 375, 144197. https://doi. org/10.1016/j.chemosphere.2025.144197 google scholar
• Zheng, Z.Y., Zhou, J.X., Peng, Z.X., & Ni, H.G. (2025). Computational simulation of bioaccumulation and trophic transfer of antibiotics mechanisms in aquatic food chain. Water Research, 272, 122951. https://doi.org/10.1016/j.watres.2024.122951 google scholar