Effects of Rutin on Bladder Contractility and Histopathology in Cyclophosphamide-Induced Hemorrhagic Cystitis in Rats

Abstract

In this study, the effects of Rutin on the isolated urinary bladder smooth muscle contractions and bladder histopathology in Cyclophosphamide (CP)-induced hemorrhagic cystitis in rats were investigated. In study, twenty-four adult female Sprague Dawley rats were used and the avarage weight of the rats was 200-250 g. Four experimental groups were formed and each group had 6 rats. The control and CP groups were administrated intraperitoneally (ip) physiologic saline solution for 7 days. Also, CP group was injected the single dose of CP (150 mg/kg, ip) on the fifth day of saline administration. The Rutin application groups, Rutin (50 mg/kg, ip) dissolved in physiological saline were injected for 7 days. The Rutin+CP group was administered a single dose of CP on the fifth day of Rutin administration. On the 8th day of the experimental procedure, the rats were decapitated in the presence of anesthesia and their bladders were isolated. The contraction values induced by Acetylcholine (ACh) (10-4 M) and Potassium Chloride (KCI) (60 mM) in isolated bladder smooth muscle strips were obtained. Besides, urinary bladders were performed the histopathologic evaluation by staining with hematoxylin eosin. It was determined that the increased in vitro contractions induced by ACh and KCl in the CP group rats but not statistically significant (P>0.05) and Rutin administration was reduced the increased contraction responses. Histopathological examination was seen that Rutin reduced these changes while epithelial rash in the bladder lumen, desquamation in the mucosal epithelium, coagulation necrosis and severe erythrocyte. In conclusion, in CP-induced hemorrhagic cystitis was determined to be a protective effect of Rutin application on bladder contractility and histopathology.

Keywords

• Cyclophosphamide,Cystitis,Histopathology,Rat,Rutin

Ratlarda Cyclophosphamide ile İndüklenen Hemorajik Sistitte Mesane Kontraktilitesi ve Histopatolojisi Üzerine Rutin’in Etkileri

Abstract

Bu çalışmada, ratlarda Cyclophosphamide (CP) ile indüklenen hemorajik sistitte izole mesane düz kas kontraksiyonları ve mesane histopatolojisi üzerine Rutin’in etkileri araştırıldı. Çalışmada 24 adet erişkin dişi Sprague Dawley rat kullanıldı ve ratların ağırlıkları ortalama 200-250 gramdı. Dört deney grubu oluşturuldu ve her bir grupta 6 rat vardı. Kontrol ve CP grubuna 7 gün intraperitoneal (ip) serum fizyolojik uygulandı. Ayrıca, CP grubuna serum fizyolojik uygulamasının 5. günü tek doz CP (150 mg/kg, ip) enjekte edildi. Rutin uygulanan gruplara serum fizyolojikte çözdürülmüş Rutin (50 mg/kg, ip) 7 gün enjekte edildi. Rutin+CP grubuna, Rutin uygulamasının 5. günü tek doz CP uygulandı. Deneysel uygulamanın 8. günü ratlar anestezi eşliğinde dekapite edilerek mesaneleri izole edildi. İzole mesane düz kas şeritlerinde Asetilkolin (Ach) (10-4 M) ve Potasyum Klorür (KCI) (60 mM) ile indüklenen kontraksiyon değerleri elde edildi. Ayrıca mesaneler hematoksilen eozin ile boyanarak histopatolojik değerlendirme yapıldı. CP grubundaki ratlarda ACh ve KCl ile indüklenen in vitro kontraksiyonların arttığı fakat istatistiksel önem arz etmediği (P>0.05) ve Rutin uygulamasının artan kontraksiyon cevaplarını azalttığı belirlendi. Histopatolojik incelemede mesane lümeninde epitel döküntü, mukoza epitelinde deskuamasyon, koagulasyon nekrozu ve şiddetli düzeyde eritrosit görülürken, Rutin’in bu değişiklikleri azalttığı görüldü. Sonuç olarak; CP ile indüklenen hemorajik sistitte mesane kontraktilitesi ve histopatolojisi üzerine Rutin uygulamasının protektif etkili olduğu belirlendi.

Keywords

• Cyclophosphamide,Histopatoloji,Rutin,Sistit,Rat

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