Keban Baraj Gölü’ndeki Tatlısu İstakozlarının (Astacus leptodactylus Esch. 1823) Dokularındaki Arginaz Aktivitesi Üzerine Kirliliğin Etkisi

Abstract

Bu çalışmada, Keban Baraj Gölü’nde bulunan Astacus leptodactylus’un dokularındaki arginaz aktivitesi üzerine kirliliğin etkisi araştırıldı. Arginaz aktivitesi, temiz bölge ile karşılaştırıldığında kirli bölgedeki kerevitlerin hepatopankreas (p < 0.05) ve solungaç (p < 0.001) dokularında daha yüksek bulundu. Kastaki arginaz seviyesinde istasyonlar arasında hiçbir fark gözlenmedi. Buna ilave olarak, kirli bölgedeki dişi kerevitlerin hepatopankreas (p < 0.001), solungaç (p < 0.01) ve kasında (p < 0.05), erkek kerevitlerin ise solungacındaki (p < 0.001) arginaz aktivitesinin istastistiksel açıdan daha yüksek olduğu tespit edildi.

Keywords

• Astacus leptodactylus, Arginaz, Keban Baraj Gölü, Kirlilik

References

1. Aminlari M., Vaseghi T., 1992. Arginase distribution in tissue of domestic animals. Comp Biochem Physiol Part B, 103(2), 385-389.
2. Anonymous., 1994. Limnological Raport of Keban Dam Lake, (in Turkish), DSİ IX. Bölge Müd. 137.
3. Borkovid SS., Pavlovid SZ., Kovačevid TB., Štajn AŠ., Petrovid VM., Saičid ZS., 2007. Antioxidant defence enzyme activities in hepatopancreas, gills and muscle of spiny cheek crayfish (Orconectes limosus) from the River Danube. Comp Biochem Physiol Part C, 147(1), 122-128.
4. Canpolat Ö., 2007. The Effects of Polluting Sources on Water Quality, Sediment and Reproduction Biology and Growth of Capoeta trutta (Heckel, 1843) in Keban Dam Lake. Fırat Unıv. Graduate School of Natural and Applied Sciences (PhD Thesis), 336.
5. Erişir M., Barim O., Özçelik M., Harlıoğlu M., 2006. The Effect of dietary vitamin E on the arginase activity in the females of freshwater crayfish (Astacus leptodactylus, Esch. 1823). Türk J Vet Anim Sci, 30, 195-199.
6. Geyer JW., Dabich D., 1971. Rapid method for determination of arginase activity in tissue homogenates. Anal Biochem, 39, 412-417.
7. Harlıoğlu MM., 2004. The Present situation of freshwater crayfish, Astacus leptodactylus (Eschscholtz, 1823) in Turkey. Aquaculture, 230, 181-187, Hartenstein R: Charecteristics of arginase from the freshwater crayfish, Cambarus bartoni. Comp Biochem Physiol Part B, 40, 781-795.
8. Lee WC., Chen JC., 2004. Nitrogenous excretion and arginase specific activity of kuruma shrimp Marsupenaeus japonicus exposed to elevated ambient nitrite. J Exp Mar Bio Ecol, 308, 103-111.

9. Livingstone DR., 2001. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar Pollut Bull, 42, 656-666.
10. Lowry OH., Rosenbrough NJ., Farr AL., Randall RJ., 1951. Protein measurements with the folin phenol reagent. J Biol Chem, 193, 265-275.
11. Muriana FJ., Ruiz-Gutierriz V., Bolufer J., 1993. Phospolipid fatty acid composition of hepatopancreas and muscle from prawn, Penaeus japonicus. J Biochem, 114, 404-407.
12. Ozan S., Gürsu MF., Sarıeyyüpoğlu M., Gülen S., 1993. The Effect of water Pollution, in Elazığ Keban Dam Lake, upon the arginase activities of the different organs of fish Capoeta trutta. Turk J Vet Anim Sci, 18, 7-10.
13. Özdamar K., 2001. SPSS ile biyoistatistik. Yayın no:3, Kaan Kitabevi, Eskişehir, 452.
14. Powers GS., Meister T. 1982. Urea synthesis and ammonia metabolism.. In: Arias I, Popper H, Schachter D, Shafrits DA Ed., the Liver: Biology and Pathobiology. Raven Pres, New York, 251-263.
15. Reddy MS., Rao KVR., Murthy BN., 1988. Changes in nitrogen metabolism of penaeid prawn, Penaeus indicus, during sublethal phosphamidon and methylparathion-induced stress. Bull Environ Contam Toxicol, 41, 344-351.