Karadeniz Bölgesinde Kene ve Sineklerle Taşınan Flavivirusların Araştırılması

Abstract

Flavivirus cinsi içinde yer alan türler dünya üzerinde halk sağlığı problemleri oluşturmaktadır. Asya’da artan Japon ensefalitis ve dang virus humması vakaları, Güney Amerika ve Afrika’da sık görülen sarı humma salgınları ve Amerika’da baştan başa yayılan batı nil virusu, flavivirusların coğrafi yoğunluğunu göstermektedir. Bu çalışmada, Karadeniz bölgesinde çeşitli memeli hayvan türlerinden (sığır, koyun, keçi ve manda) toplanan 2340 olgun kene (675 kene havuzu) ve Culex spp., Anopheles spp., Aedes spp., ve Culicoides spp.’den oluşan 3226 sinek (142 sinek havuzu) sinek ve kenelerle taşınan flavivirusların varlığı yönünden reverz-transkriptaz zincir reaksiyonu metodu ile test edildi. Örneklerin hiçbirisinde flavivirus genomik RNA’sı tespit edilemedi. Bu çalışma, hem kene hem de sineklerle taşınan flavivirusların varlığı yönünden Türkiye’de yapılan ilk çalışmadır.

Keywords

• Kene, RT-PZR, Sinek, Türkiye, Vektör-taşıyıcılı flavivirus

Investigation of Tick- and Mosquito-Borne Flaviviruses in Blacksea Region

Abstract

Species within the Flavivirus genus pose public health problems around the world. Increasing cases of Dengue and Japanese encephalistis virus in Asia, frequent outbreaks of Yellow fever virus in Africa and South America, and the ongoing spread of West Nile virus throughout the America, show the geographical burden of flavivirus diseases. In this study, a total of 2340 adult ticks (675 hard tick pools) collected from the variety of mammalian species (cattle, sheep, goat and buffalo) and 3226 mosquitoes (142 mosquito pools) including Culex spp., Anopheles spp., Aedes spp., and Culicoides spp., trapped in Blacksea region of northern Turkey were surveyed for the presence of RNA from mosquito-borne flaviviruses (MBFV) and tick-borne flaviviruses (TBFV) by reverse transcriptase-polymerase chain reaction (RT-PCR) assay. No flavivirus genomic RNA was detected in these samples. This is the first study about both the TBFV and MBFV infections in Turkey.

Keywords

• Mosquito, RT-PCR, Tick, Turkey, Vector-borne flavivirus.

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